MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 182)


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+71

Accession Score Description
1 ATPG_PSEPG 70 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

-72

Accession Score Description
1 HYDA_PSEAE 69 D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1
Score Mass Matches Sequences emPAI
72.1 HYDA_PSEAE 69 52521 3 (2) 3 (2) 0.09
D-hydantoinase/dihydropyrimidinase OS=Pseudomonas aeruginosa GN=dht PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
934   453.7691 905.5236 905.5123 12.5 0 35 0.1 +1Score > 38 indicates identity U K.IFNLFPR.K
1482   571.7958 1141.5770 1141.5727 3.77 1 63 0.00034 +1Score > 40 indicates identity U K.IPNGTAGIEDR.M
2981   624.0290 1869.0652 1869.0360 15.6 1 37 0.0013 +1Score > 32 indicates identity
Score > 20 indicates homology
U R.IAETLGTPLYLVHISSR.E

1 subset or intersection (1 subset protein in total)

Score Mass Subset of
HYDA_PSEPU 63 53872 72.1
D-hydantoinase/dihydropyrimidinase OS=Pseudomonas putida GN=dht PE=1 SV=2

+73

Accession Score Description
1 RL10_PSEPG 69 50S ribosomal protein L10 OS=Pseudomonas putida (strain GB-1) GN=rplJ PE=3 SV=1

+74

Accession Score Description
1 RL15_PSEP1 67 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1

+75

Accession Score Description
1 RL14_AZOSE 61 50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1

+76

Accession Score Description
1 ODP1_MYCTU 66 Pyruvate dehydrogenase E1 component OS=Mycobacterium tuberculosis GN=aceE PE=3 SV=1

+77

Accession Score Description
1 PURA_PSEPG 64 Adenylosuccinate synthetase OS=Pseudomonas putida (strain GB-1) GN=purA PE=3 SV=1

+78

Accession Score Description
1 SYD_AZOVD 62 Aspartyl-tRNA synthetase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=aspS PE=3 SV=1

+79

Accession Score Description
1 GLNA_AZOVI 62 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1

+80

Accession Score Description
1 CH10_PSE14 61 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
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