MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 182)

Page: Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 19 Next 

+61

Accession Score Description
1 SUCD_PSEAE 75 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+62

Accession Score Description
1 RS20_PSE14 75 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+63

Accession Score Description
1 RRF_ACTSZ 75 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+64

Accession Score Description
1 RL29_PSE14 75 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+65

Accession Score Description
1 OPRI_PSEAE 74 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+66

Accession Score Description
1 RL16_PSEP1 74 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+67

Accession Score Description
1 CISY_PSEAE 73 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+68

Accession Score Description
1 IF2_CAMHC 71 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+69

Accession Score Description
1 RL21_PSEE4 71 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

-70

Accession Score Description
1 FLAA_VIBPA 71 Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
Score Mass Matches Sequences emPAI
70.1 FLAA_VIBPA 71 39825 5 (2) 3 (1) 0.06
Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
19 samesets of FLAA_VIBPA
FLAA_VIBAN 71 40087 3 (2) 2 (1) 0.06
Flagellin A OS=Vibrio anguillarum GN=flaA PE=3 SV=1
FLAA_VIBCH 71 40358 3 (2) 2 (1) 0.06
Flagellin A OS=Vibrio cholerae GN=flaA PE=3 SV=1
FLAB_VIBPA 71 40149 3 (2) 2 (1) 0.06
Polar flagellin B/D OS=Vibrio parahaemolyticus GN=flaB PE=3 SV=2
FLAC_VIBPA 71 40787 3 (2) 2 (1) 0.06
Polar flagellin C OS=Vibrio parahaemolyticus GN=flaC PE=3 SV=2
FLAD_VIBAN 71 39617 3 (2) 2 (1) 0.06
Flagellin D OS=Vibrio anguillarum GN=flaD PE=1 SV=3
FLAE_VIBAN 71 40757 3 (2) 2 (1) 0.06
Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1
FLAE_VIBCH 71 41017 3 (2) 2 (1) 0.06
Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
FLICB_PSEAE 71 49213 3 (2) 2 (1) 0.05
B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
FLAA_VIBC3 71 40358 3 (2) 2 (1) 0.06
Flagellin A OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaA PE=3 SV=1
FLAE_VIBC3 71 41017 3 (2) 2 (1) 0.06
Flagellin E OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaE PE=3 SV=1
FLAC_VIBCH 71 39881 3 (2) 2 (1) 0.06
Flagellin C OS=Vibrio cholerae GN=flaC PE=3 SV=1
FLAD_VIBCH 71 39880 3 (2) 2 (1) 0.06
Flagellin D OS=Vibrio cholerae GN=flaD PE=1 SV=1
FLAC_VIBC3 71 39881 3 (2) 2 (1) 0.06
Flagellin C OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaC PE=3 SV=1
FLAD_VIBC3 71 39880 3 (2) 2 (1) 0.06
Flagellin D OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaD PE=3 SV=1
FLAF_VIBPA 71 40476 3 (2) 2 (1) 0.06
Polar flagellin F OS=Vibrio parahaemolyticus GN=flaF PE=3 SV=2
FLAB_VIBAN 71 39509 2 (2) 1 (1) 0.06
Flagellin B OS=Vibrio anguillarum GN=flaB PE=1 SV=3
FLAB_VIBCH 71 39492 2 (2) 1 (1) 0.06
Flagellin B OS=Vibrio cholerae GN=flaB PE=3 SV=1
FLAB_VIBC3 71 39492 2 (2) 1 (1) 0.06
Flagellin B OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaB PE=3 SV=1
FLAC_VIBAN 71 40076 2 (2) 1 (1) 0.06
Flagellin C OS=Vibrio anguillarum GN=flaC PE=1 SV=3

-5 peptide matches (3 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1521 +1 580.2905 1158.5664 1158.5629 3.07 0 65 0.00025 +1Score > 41 indicates identity U K.DDAAGLQISNR.L
2115 +1 712.3593 1422.7040 1422.6878 11.4 1 8 2.3 +7Score > 41 indicates identity
Score > 24 indicates homology		 U K.LQVFASTQKVNGE.V + 3 Deamidated (NQ)
2651   558.6281 1672.8625 1672.8380 14.6 1 16 0.44 +1Score > 40 indicates identity
Score > 25 indicates homology		 U K.INSAKDDAAGLQISNR.L + Deamidated (NQ)
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