MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 75 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 75 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RRF_ACTSZ | 75 | Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL29_PSE14 | 75 | 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1 |
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPRI_PSEAE | 74 | Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEP1 | 74 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_PSEAE | 73 | Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2 |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_CAMHC | 71 | Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1 |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 71 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 69.1 | RL21_PSEE4 |
71 | 11596 | 8 (3) | 4 (2) | 0.46 | |
| 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 | |||||||
| 4 samesets of RL21_PSEE4 | |||||||
| RL21_PSEP1 |
71 | 11624 | 8 (3) | 4 (2) | 0.46 | ||
| 50S ribosomal protein L21 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplU PE=3 SV=1 | |||||||
| RL21_PSEPG |
71 | 11624 | 8 (3) | 4 (2) | 0.46 | ||
| 50S ribosomal protein L21 OS=Pseudomonas putida (strain GB-1) GN=rplU PE=3 SV=1 | |||||||
| RL21_PSEPK |
71 | 11624 | 8 (3) | 4 (2) | 0.46 | ||
| 50S ribosomal protein L21 OS=Pseudomonas putida (strain KT2440) GN=rplU PE=3 SV=2 | |||||||
| RL21_PSEPW |
71 | 11638 | 8 (3) | 4 (2) | 0.46 | ||
| 50S ribosomal protein L21 OS=Pseudomonas putida (strain W619) GN=rplU PE=3 SV=1 | |||||||
8 peptide matches (4 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 318 |
1 |
602.3526 | 601.3453 | 601.3435 | 3.00 | 0 | 19 | 1.6 | 6Score > 45 indicates identity Score > 34 indicates homology |
K.ITGIQA.- | |
| 1058 |
1 |
476.2551 | 950.4956 | 950.4862 | 9.98 | 1 | 29 | 0.31 | 1Score > 40 indicates identity Score > 37 indicates homology |
R.QWFTEIK.I | |
| 1151 |
1 |
497.7842 | 993.5538 | 993.5495 | 4.38 | 0 | 31 | 0.013 | 1Score > 38 indicates identity Score > 25 indicates homology |
U | M.SYAVIVTGGK.Q |
| 1274 |
1 |
522.2977 | 1042.5808 | 1042.5771 | 3.58 | 1 | 58 | 0.00045 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | K.VVAEVVSQGR.H |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL21_HAES1 |
69 | 11492 | 69.1 | ||
| 50S ribosomal protein L21 OS=Haemophilus somnus (strain 129Pt) GN=rplU PE=3 SV=1 | |||||
| 2 samesets of RL21_HAES1 | |||||
| RL21_PSEMY |
69 | 11605 | |||
| 50S ribosomal protein L21 OS=Pseudomonas mendocina (strain ymp) GN=rplU PE=3 SV=1 | |||||
| RL21_HAES2 |
69 | 11492 | |||
| 50S ribosomal protein L21 OS=Haemophilus somnus (strain 2336) GN=rplU PE=3 SV=1 | |||||
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLAA_VIBPA | 71 | Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1 |
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