MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCD_PSEAE | 75 | Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 75 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RRF_ACTSZ | 75 | Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL29_PSE14 | 75 | 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1 |
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPRI_PSEAE | 74 | Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL16_PSEP1 | 74 | 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CISY_PSEAE | 73 | Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 67.1 | CISY_PSEAE |
73 | 48006 | 4 (2) | 2 (1) | 0.10 | |
| Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2 | |||||||
| 24 samesets of CISY_PSEAE |
|||||||
4 peptide matches (4 non-duplicate, 0 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 975 |
462.7390 | 923.4634 | 923.4600 | 3.73 | 0 | 21 | 0.082 | 1Score > 41 indicates identity Score > 22 indicates homology |
K.ITYIDGDK.G | ||
| 3228 |
510.5147 | 2038.0297 | 2038.0232 | 3.19 | 1 | 29 | 0.018 | 1Score > 39 indicates identity Score > 24 indicates homology |
U | R.IFILHADHEQNASTSTVR.L | |
| 3232 |
510.7658 | 2039.0341 | 2039.0072 | 13.2 | 1 | 16 | 0.41 | 1Score > 39 indicates identity Score > 25 indicates homology |
U | R.IFILHADHEQNASTSTVR.L + Deamidated (NQ) | |
| 3233 |
680.6857 | 2039.0353 | 2039.0072 | 13.8 | 1 | 68 | 2.8e-006 | 1Score > 39 indicates identity Score > 25 indicates homology |
U | R.IFILHADHEQNASTSTVR.L + Deamidated (NQ) |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IF2_CAMHC | 71 | Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1 |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 71 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLAA_VIBPA | 71 | Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1 |
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