MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 182)


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+61

Accession Score Description
1 SUCD_PSEAE 75 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+62

Accession Score Description
1 RS20_PSE14 75 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+63

Accession Score Description
1 RRF_ACTSZ 75 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

+64

Accession Score Description
1 RL29_PSE14 75 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+65

Accession Score Description
1 OPRI_PSEAE 74 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

-66

Accession Score Description
1 RL16_PSEP1 74 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1
Score Mass Matches Sequences emPAI
66.1 RL16_PSEP1 74 15364 7 (5) 5 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1
11 samesets of RL16_PSEP1
RL16_PSEPG 74 15364 7 (5) 5 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain GB-1) GN=rplP PE=3 SV=1
RL16_PSEPK 74 15364 7 (5) 5 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1
RL16_PSEPW 74 15364 7 (5) 5 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain W619) GN=rplP PE=3 SV=1
RL16_PSEE4 74 15364 7 (5) 5 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas entomophila (strain L48) GN=rplP PE=3 SV=1
RL16_PSEU5 74 15395 7 (5) 5 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas stutzeri (strain A1501) GN=rplP PE=3 SV=1
RL16_PSEF5 74 15407 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1
RL16_PSEFS 74 15422 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain SBW25) GN=rplP PE=3 SV=1
RL16_PSEPF 74 15407 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplP PE=3 SV=1
RL16_PSE14 74 15421 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplP PE=3 SV=1
RL16_PSESM 74 15421 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. tomato GN=rplP PE=3 SV=1
RL16_PSEU2 74 15421 6 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplP PE=3 SV=1

+7 peptide matches (5 non-duplicate, 2 duplicate)

4 subsets and intersections (37 subset proteins in total)

Score Mass Subset of
RL16_PSEA7 60 15362 66.1
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain PA7) GN=rplP PE=3 SV=1
3 samesets of RL16_PSEA7
RL16_PSEA8 60 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplP PE=3 SV=1
RL16_PSEAB 60 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplP PE=3 SV=1
RL16_PSEAE 60 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa GN=rplP PE=3 SV=1
RL16_AZOVD 52 15445 66.1
50S ribosomal protein L16 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplP PE=3 SV=1
1 sameset of RL16_AZOVD
RL16_PSEMY 52 15379
50S ribosomal protein L16 OS=Pseudomonas mendocina (strain ymp) GN=rplP PE=3 SV=1
RL16_ACIB3 43 15514 66.1
50S ribosomal protein L16 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplP PE=3 SV=1
+29 samesets of RL16_ACIB3
RL16_SACD2 43 15613 66.1
50S ribosomal protein L16 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplP PE=3 SV=1

+67

Accession Score Description
1 CISY_PSEAE 73 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+68

Accession Score Description
1 IF2_CAMHC 71 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+69

Accession Score Description
1 RL21_PSEE4 71 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+70

Accession Score Description
1 FLAA_VIBPA 71 Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
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