Rita4 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita4 sp
MS data file	:	PRT1270_T-BRSC_4_20250714120925.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:41:34 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita4 sp

MS data file

PRT1270_T-BRSC_4_20250714120925.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:41:34 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,786

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,786

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

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Protein Family Summary

Protein families 61–70 (out of 182)

Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 19 Next

61

		Accession	Score	Description
	1	SUCD_PSEAE	75	Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

62

		Accession	Score	Description
	1	RS20_PSE14	75	30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

63

		Accession	Score	Description
	1	RRF_ACTSZ	75	Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

64

		Accession	Score	Description
	1	RL29_PSE14	75	50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

65

		Accession	Score	Description
	1	OPRI_PSEAE	74	Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

66

		Accession	Score	Description
	1	RL16_PSEP1	74	50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
66.1	RL16_PSEP1	74	15364	7 (5)	5 (3)	0.54
	50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1
	11 samesets of RL16_PSEP1
	RL16_PSEPG	74	15364	7 (5)	5 (3)	0.54
	50S ribosomal protein L16 OS=Pseudomonas putida (strain GB-1) GN=rplP PE=3 SV=1
	RL16_PSEPK	74	15364	7 (5)	5 (3)	0.54
	50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1
	RL16_PSEPW	74	15364	7 (5)	5 (3)	0.54
	50S ribosomal protein L16 OS=Pseudomonas putida (strain W619) GN=rplP PE=3 SV=1
	RL16_PSEE4	74	15364	7 (5)	5 (3)	0.54
	50S ribosomal protein L16 OS=Pseudomonas entomophila (strain L48) GN=rplP PE=3 SV=1
	RL16_PSEU5	74	15395	7 (5)	5 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas stutzeri (strain A1501) GN=rplP PE=3 SV=1
	RL16_PSEF5	74	15407	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1
	RL16_PSEFS	74	15422	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain SBW25) GN=rplP PE=3 SV=1
	RL16_PSEPF	74	15407	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplP PE=3 SV=1
	RL16_PSE14	74	15421	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplP PE=3 SV=1
	RL16_PSESM	74	15421	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas syringae pv. tomato GN=rplP PE=3 SV=1
	RL16_PSEU2	74	15421	6 (5)	4 (3)	0.53
	50S ribosomal protein L16 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplP PE=3 SV=1

7 peptide matches (5 non-duplicate, 2 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
899		446.2530	890.4914	890.4861	5.97	1	43	0.02	1Score > 40 indicates identity Score > 39 indicates homology	U	R.EAFALAAAK.L
1117	1	488.3018	974.5890	974.5801	9.22	0	53	0.00053	1Score > 33 indicates identity	U	K.LPLATSFVK.R
1165	1	499.2772	996.5398	996.5280	11.9	1	35	0.0049	1Score > 38 indicates identity Score > 24 indicates homology	U	K.VSFGEFALK.A
1240		344.2037	1029.5893	1029.5859	3.30	0	28	0.64	1Score > 40 indicates identity Score > 38 indicates homology	U	R.VFPDKPISK.K
2385		781.4117	1560.8088	1560.7936	9.74	1	12	0.62	1Score > 41 indicates identity Score > 22 indicates homology		K.GSVEYWVAQIQPGK.V

4 subsets and intersections (37 subset proteins in total)

	Score	Mass	Subset of
RL16_PSEA7	60	15362	66.1
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain PA7) GN=rplP PE=3 SV=1
3 samesets of RL16_PSEA7
RL16_PSEA8	60	15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplP PE=3 SV=1
RL16_PSEAB	60	15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplP PE=3 SV=1
RL16_PSEAE	60	15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa GN=rplP PE=3 SV=1
RL16_AZOVD	52	15445	66.1
50S ribosomal protein L16 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplP PE=3 SV=1
1 sameset of RL16_AZOVD
RL16_PSEMY	52	15379
50S ribosomal protein L16 OS=Pseudomonas mendocina (strain ymp) GN=rplP PE=3 SV=1
RL16_ACIB3	43	15514	66.1
50S ribosomal protein L16 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplP PE=3 SV=1
29 samesets of RL16_ACIB3
RL16_SACD2	43	15613	66.1
50S ribosomal protein L16 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplP PE=3 SV=1

67

		Accession	Score	Description
	1	CISY_PSEAE	73	Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

68

		Accession	Score	Description
	1	IF2_CAMHC	71	Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

69

		Accession	Score	Description
	1	RL21_PSEE4	71	50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

70

		Accession	Score	Description
	1	FLAA_VIBPA	71	Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1

Previous 1 …2 3 4 5 6 7 8 9 10 11 12 … 19 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 61–70 (out of 182)

61

62

63

64

65

66

7 peptide matches (5 non-duplicate, 2 duplicate)

4 subsets and intersections (37 subset proteins in total)

67

68

69

70