MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 182)


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+61

Accession Score Description
1 SUCD_PSEAE 75 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+62

Accession Score Description
1 RS20_PSE14 75 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+63

Accession Score Description
1 RRF_ACTSZ 75 Ribosome-recycling factor OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=frr PE=3 SV=1

-64

Accession Score Description
1 RL29_PSE14 75 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1
Score Mass Matches Sequences emPAI
64.1 RL29_PSE14 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1
10 samesets of RL29_PSE14
RL29_PSEE4 75 7299 1 (1) 1 (1) 0.34
50S ribosomal protein L29 OS=Pseudomonas entomophila (strain L48) GN=rpmC PE=3 SV=1
RL29_PSEF5 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpmC PE=3 SV=1
RL29_PSEFS 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas fluorescens (strain SBW25) GN=rpmC PE=3 SV=1
RL29_PSEP1 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmC PE=3 SV=1
RL29_PSEPF 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpmC PE=3 SV=1
RL29_PSEPG 75 7299 1 (1) 1 (1) 0.34
50S ribosomal protein L29 OS=Pseudomonas putida (strain GB-1) GN=rpmC PE=3 SV=1
RL29_PSEPK 75 7299 1 (1) 1 (1) 0.34
50S ribosomal protein L29 OS=Pseudomonas putida (strain KT2440) GN=rpmC PE=3 SV=1
RL29_PSEPW 75 7299 1 (1) 1 (1) 0.34
50S ribosomal protein L29 OS=Pseudomonas putida (strain W619) GN=rpmC PE=3 SV=1
RL29_PSESM 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas syringae pv. tomato GN=rpmC PE=3 SV=1
RL29_PSEU2 75 7168 1 (1) 1 (1) 0.35
50S ribosomal protein L29 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpmC PE=3 SV=1

-1 peptide matches (1 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
2443   791.9605 1581.9064 1581.8838 14.3 1 75 6.9e-007 +1Score > 34 indicates identity
Score > 26 indicates homology
U K.SAQQLNEQLLGLLR.D

+65

Accession Score Description
1 OPRI_PSEAE 74 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+66

Accession Score Description
1 RL16_PSEP1 74 50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1

+67

Accession Score Description
1 CISY_PSEAE 73 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+68

Accession Score Description
1 IF2_CAMHC 71 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+69

Accession Score Description
1 RL21_PSEE4 71 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1

+70

Accession Score Description
1 FLAA_VIBPA 71 Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
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