MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1194 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 3 | EFTU_CARRP | 163 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1113 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 941 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSESM | 569 | ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 882 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 169 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 206 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 450 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 70 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 115 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 402 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 364 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | RPOC_PSEPK |
364 | 155386 | 30 (19) | 23 (14) | 0.22 | |
| DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 | |||||||
| 4 samesets of RPOC_PSEPK | |||||||
| RPOC_PSEP1 |
364 | 155358 | 30 (19) | 23 (14) | 0.22 | ||
| DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoC PE=3 SV=1 | |||||||
| RPOC_PSEPG |
364 | 155435 | 29 (19) | 22 (14) | 0.22 | ||
| DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain GB-1) GN=rpoC PE=3 SV=1 | |||||||
| RPOC_PSEPW |
364 | 155341 | 29 (19) | 22 (14) | 0.23 | ||
| DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain W619) GN=rpoC PE=3 SV=1 | |||||||
| RPOC_PSEE4 |
364 | 155376 | 29 (19) | 22 (14) | 0.22 | ||
| DNA-directed RNA polymerase subunit beta' OS=Pseudomonas entomophila (strain L48) GN=rpoC PE=3 SV=1 | |||||||
30 peptide matches (23 non-duplicate, 7 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 257 |
517.3021 | 516.2948 | 516.2907 | 7.94 | 0 | 20 | 0.78 | 3Score > 48 indicates identity Score > 31 indicates homology |
E.AINAK.G + Deamidated (NQ) | ||
| 602 |
1 |
372.2566 | 742.4986 | 742.4953 | 4.55 | 0 | 21 | 0.11 | 1Score > 32 indicates identity Score > 24 indicates homology |
U | R.VLLGITK.A |
| 847 |
430.7479 | 859.4812 | 859.4763 | 5.70 | 0 | 13 | 11 | 9Score > 42 indicates identity Score > 36 indicates homology |
U | R.IVDTTVGR.A | |
| 849 |
1 |
430.7507 | 859.4868 | 859.4803 | 7.56 | 0 | 38 | 0.062 | 1Score > 41 indicates identity Score > 38 indicates homology |
U | K.VIDLWSK.A |
| 863 |
436.2873 | 870.5600 | 870.5538 | 7.14 | 0 | 40 | 0.011 | 1Score > 33 indicates identity Score > 33 indicates homology |
U | R.LLGVSALAK.Y | |
| 957 |
458.7791 | 915.5436 | 915.5389 | 5.18 | 1 | 61 | 0.00035 | 1Score > 39 indicates identity |
U | R.KGLADTALK.T | |
| 963 |
460.7492 | 919.4838 | 919.4763 | 8.19 | 1 | 19 | 0.48 | 1Score > 42 indicates identity Score > 28 indicates homology |
U | R.VADLFEAR.R | |
| 1070 |
319.8684 | 956.5834 | 956.5906 | -7.55 | 1 | 13 | 2.8 | 6Score > 39 indicates identity Score > 30 indicates homology |
U | M.KDLLNLLK.N + Deamidated (NQ) | |
| 1139 |
494.7902 | 987.5658 | 987.5601 | 5.86 | 1 | 39 | 0.057 | 1Score > 39 indicates identity |
U | R.VLTEAAVTGK.R | |
| 1174 |
501.2756 | 1000.5366 | 1000.5302 | 6.49 | 0 | 44 | 0.0074 | 1Score > 42 indicates identity Score > 36 indicates homology |
U | R.ADGNLVAVSR.S | |
| 1216 |
339.5152 | 1015.5238 | 1015.5199 | 3.79 | 0 | 29 | 0.47 | 1Score > 42 indicates identity Score > 38 indicates homology |
U | R.TFHIGGAASR.T | |
| 1272 |
521.8209 | 1041.6272 | 1041.6183 | 8.63 | 0 | 46 | 0.0076 | 1Score > 38 indicates identity |
U | R.SVITVGPTLR.L | |
| 1302 |
530.2902 | 1058.5658 | 1058.5608 | 4.79 | 1 | 40 | 0.092 | 1Score > 42 indicates identity |
U | R.IIGNATDEVK.E | |
| 1608 |
596.3075 | 1190.6004 | 1190.5932 | 6.13 | 1 | 34 | 0.025 | 1Score > 42 indicates identity Score > 31 indicates homology |
U | R.VFADLQEVDR.V | |
| 1885 |
1 |
657.8249 | 1313.6352 | 1313.6252 | 7.67 | 0 | 35 | 0.01 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | R.FATSDLNDLYR.R |
| 1916 |
1 |
662.9041 | 1323.7936 | 1323.7762 | 13.2 | 0 | 62 | 4.9e-005 | 1Score > 32 indicates identity |
U | R.LLDLSAPDIIVR.N |
| 1938 |
446.6027 | 1336.7863 | 1336.7755 | 8.09 | 1 | 55 | 8e-005 | 1Score > 34 indicates identity Score > 26 indicates homology |
U | R.YKLPYGAVISVK.E | |
| 2088 |
1 |
706.3698 | 1410.7250 | 1410.7143 | 7.61 | 1 | 68 | 9.5e-005 | 1Score > 40 indicates identity |
U | K.YIVNEIQDVYR.L |
| 2223 |
495.6021 | 1483.7845 | 1483.7783 | 4.16 | 1 | 58 | 7e-005 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | R.LIPAGTGLAYHSER.K | |
| 2295 |
1 |
757.4457 | 1512.8768 | 1512.8552 | 14.3 | 1 | 62 | 9.1e-005 | 1Score > 34 indicates identity |
U | R.LGIQAFEPVLIEGK.A |
| 2409 |
393.9615 | 1571.8169 | 1571.8096 | 4.62 | 1 | 13 | 0.15 | 1Score > 40 indicates identity Score > 17 indicates homology |
U | K.WDPHTHPIVTELK.G | |
| 2700 |
569.6186 | 1705.8340 | 1705.8271 | 4.01 | 1 | 63 | 1.3e-005 | 1Score > 41 indicates identity Score > 26 indicates homology |
U | R.GEVISDGPSDPHDILR.L | |
| 2826 |
1 |
593.3213 | 1776.9421 | 1776.9159 | 14.7 | 1 | 56 | 9e-005 | 1Score > 39 indicates identity Score > 28 indicates homology |
U | R.EGLSVLQYFISTHGAR.K |
24 subsets and intersections (630 subset proteins in total)7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 308 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 290 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 78 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEP1 | 287 | 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSE14 | 192 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 280 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
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