MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 182)

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+51

Accession Score Description
1 CYOA_PSEPU 89 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 87 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+53

Accession Score Description
1 RL6_PSEP1 87 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+54

Accession Score Description
1 ODO1_AZOVI 85 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+55

Accession Score Description
1 K2C1_HUMAN 83 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+56

Accession Score Description
1 KPRS_PSEPK 83 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

-57

Accession Score Description
1 SYA_PSEP1 81 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2
Score Mass Matches Sequences emPAI
57.1 SYA_PSEP1 81 95108 7 (5) 5 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2
2 samesets of SYA_PSEP1
SYA_PSEPG 81 95050 7 (5) 5 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2
SYA_PSEPK 81 95109 7 (5) 5 (4) 0.10
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain KT2440) GN=alaS PE=3 SV=1

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1074   479.7720 957.5294 957.5131 17.1 1 21 0.42 +1Score > 41 indicates identity
Score > 30 indicates homology		 U K.QLEQLQAK.A + Deamidated (NQ)
1346 +1 542.3489 1082.6832 1082.6699 12.3 0 57 0.00016 +1Score > 32 indicates identity U K.ALLALVDQLK.N
1492   572.8229 1143.6312 1143.6248 5.65 1 46 0.0036 +1Score > 41 indicates identity
Score > 34 indicates homology		 U K.IISEGGVASGVR.R
1552   585.8461 1169.6776 1169.6656 10.3 1 45 0.011 +1Score > 38 indicates identity U R.ILEQDLAQLK.G
4192   951.5205 2851.5397 2851.4868 18.5 1 11 1 +1Score > 33 indicates identity
Score > 23 indicates homology		 U R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ)
4193   713.8922 2851.5397 2851.4868 18.5 1 17 0.027 +1Score > 33 indicates identity
Score > 14 indicates homology		 U R.VSAVLQHVHSNYEIDLFQNLLAAAAK.A + Deamidated (NQ)

3 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
SYA_PSEPW 82 94991 57.1
Alanyl-tRNA synthetase OS=Pseudomonas putida (strain W619) GN=alaS PE=3 SV=2
SYA_PSEE4 74 95309 57.1
Alanyl-tRNA synthetase OS=Pseudomonas entomophila (strain L48) GN=alaS PE=3 SV=1
1 sameset of SYA_PSEE4
SYA_PSEPF 74 94840
Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf0-1) GN=alaS PE=3 SV=1
SYA_PSE14 61 94784 57.1
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=alaS PE=3 SV=1
8 samesets of SYA_PSE14
SYA_PSESM 61 94865
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. tomato GN=alaS PE=3 SV=1
SYA_PSEU2 61 94751
Alanyl-tRNA synthetase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=alaS PE=3 SV=2
SYA_PSEF5 61 95022
Alanyl-tRNA synthetase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=alaS PE=3 SV=1
SYA_PSEMY 61 94797
Alanyl-tRNA synthetase OS=Pseudomonas mendocina (strain ymp) GN=alaS PE=3 SV=2
SYA_PSEAB 61 95112
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=alaS PE=3 SV=1
SYA_PSEAE 61 95099
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa GN=alaS PE=3 SV=1
SYA_PSEU5 61 95029
Alanyl-tRNA synthetase OS=Pseudomonas stutzeri (strain A1501) GN=alaS PE=3 SV=2
SYA_PSEA7 61 94997
Alanyl-tRNA synthetase OS=Pseudomonas aeruginosa (strain PA7) GN=alaS PE=3 SV=1

+58

Accession Score Description
1 ASPQ_PSEPK 80 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+59

Accession Score Description
1 RL7_PSEP1 76 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+60

Accession Score Description
1 YJJK_ECOLI 76 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2
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