MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 182)


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+51

Accession Score Description
1 CYOA_PSEPU 89 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 87 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+53

Accession Score Description
1 RL6_PSEP1 87 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+54

Accession Score Description
1 ODO1_AZOVI 85 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

-55

Accession Score Description
1 K2C1_HUMAN 83 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
Score Mass Matches Sequences emPAI
55.1 K2C1_HUMAN 83 66170 3 (2) 3 (2) 0.07
Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6
1 sameset of K2C1_HUMAN
K2C1_PANTR 83 65621 3 (2) 3 (2) 0.07
Keratin, type II cytoskeletal 1 OS=Pan troglodytes GN=KRT1 PE=2 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1865   651.8699 1301.7252 1301.7078 13.4 1 68 5.7e-005 +1Score > 39 indicates identity
Score > 38 indicates homology
U R.SLDLDSIIAEVK.A
2017   692.3551 1382.6956 1382.6830 9.14 0 8 0.74 +1Score > 40 indicates identity
Score > 19 indicates homology
U K.SLNNQFASFIDK.V
2203   738.4014 1474.7882 1474.7780 6.96 1 53 0.0018 +1Score > 40 indicates identity
Score > 38 indicates homology
U R.FLEQQNQVLQTK.W

2 subsets and intersections (21 subset proteins in total)

Score Mass Subset of
K2C5_BOVIN 68 63069 55.1
Keratin, type II cytoskeletal 5 OS=Bos taurus GN=KRT5 PE=1 SV=1
9 samesets of K2C5_BOVIN
K2C5_MOUSE 68 61957
Keratin, type II cytoskeletal 5 OS=Mus musculus GN=Krt5 PE=1 SV=1
K2C5_RAT 68 61959
Keratin, type II cytoskeletal 5 OS=Rattus norvegicus GN=Krt5 PE=1 SV=1
K2C6A_MOUSE 68 59641
Keratin, type II cytoskeletal 6A OS=Mus musculus GN=Krt6a PE=2 SV=3
K2C6A_RAT 68 59555
Keratin, type II cytoskeletal 6A OS=Rattus norvegicus GN=Krt6a PE=1 SV=1
K2C6B_MOUSE 68 60627
Keratin, type II cytoskeletal 6B OS=Mus musculus GN=Krt6b PE=1 SV=3
K2C75_BOVIN 68 59399
Keratin, type II cytoskeletal 75 OS=Bos taurus GN=KRT75 PE=2 SV=1
K2C75_MOUSE 68 59932
Keratin, type II cytoskeletal 75 OS=Mus musculus GN=Krt75 PE=1 SV=1
K2C75_RAT 68 59219
Keratin, type II cytoskeletal 75 OS=Rattus norvegicus GN=Krt75 PE=2 SV=2
K2C7_BOVIN 68 51603
Keratin, type II cytoskeletal 7 OS=Bos taurus GN=KRT7 PE=2 SV=1
K22E_CANFA 53 64812 55.1
Keratin, type II cytoskeletal 2 epidermal OS=Canis familiaris GN=KRT2 PE=2 SV=1
10 samesets of K22E_CANFA
K2C1_CANFA 53 63922
Keratin, type II cytoskeletal 1 OS=Canis familiaris GN=KRT1 PE=2 SV=1
K2C1B_MOUSE 53 61379
Keratin, type II cytoskeletal 1b OS=Mus musculus GN=Krt77 PE=1 SV=1
K2C1B_RAT 53 57391
Keratin, type II cytoskeletal 1b OS=Rattus norvegicus GN=Krt77 PE=2 SV=1
K2C1_MOUSE 53 66079
Keratin, type II cytoskeletal 1 OS=Mus musculus GN=Krt1 PE=1 SV=4
K2C1_RAT 53 65190
Keratin, type II cytoskeletal 1 OS=Rattus norvegicus GN=Krt1 PE=2 SV=1
K2C71_MOUSE 53 57860
Keratin, type II cytoskeletal 71 OS=Mus musculus GN=Krt71 PE=1 SV=1
K2C73_MOUSE 53 59502
Keratin, type II cytoskeletal 73 OS=Mus musculus GN=Krt73 PE=1 SV=1
K2C73_RAT 53 60977
Keratin, type II cytoskeletal 73 OS=Rattus norvegicus GN=Krt73 PE=1 SV=1
K2C74_MOUSE 53 55340
Keratin, type II cytoskeletal 74 OS=Mus musculus GN=Krt74 PE=2 SV=1
K22E_HUMAN 53 65678
Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2

+56

Accession Score Description
1 KPRS_PSEPK 83 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+57

Accession Score Description
1 SYA_PSEP1 81 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2

+58

Accession Score Description
1 ASPQ_PSEPK 80 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+59

Accession Score Description
1 RL7_PSEP1 76 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+60

Accession Score Description
1 YJJK_ECOLI 76 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2
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