MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 182)

Page: Previous 1 2 3 4 5 6 7 8 9 10 11 … 19 Next 

+51

Accession Score Description
1 CYOA_PSEPU 89 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+52

Accession Score Description
1 DHSB_ECOLI 87 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

-53

Accession Score Description
1 RL6_PSEP1 87 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
Score Mass Matches Sequences emPAI
53.1 RL6_PSEP1 87 19131 7 (4) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
6 samesets of RL6_PSEP1
RL6_PSEPG 87 19131 7 (4) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain GB-1) GN=rplF PE=3 SV=1
RL6_PSEPK 87 19131 7 (4) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1
RL6_PSEF5 87 19170 6 (4) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplF PE=3 SV=1
RL6_PSEFS 87 19185 6 (4) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain SBW25) GN=rplF PE=3 SV=1
RL6_PSEPF 87 19184 6 (4) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplF PE=3 SV=1
RL6_PSEPW 87 19115 6 (4) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain W619) GN=rplF PE=3 SV=1

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
763   414.7476 827.4806 827.4753 6.50 1 19 1.2 +3Score > 40 indicates identity
Score > 32 indicates homology		 K.LPSGVEVK.F
816   426.2190 850.4234 850.4185 5.85 1 7 0.88 +1Score > 42 indicates identity
Score > 19 indicates homology		 R.YADEVVR.R
1121 +1 489.2761 976.5376 976.5342 3.55 0 47 0.014 +1Score > 41 indicates identity U K.FAGQQLSVK.G
1387   552.8463 1103.6780 1103.6703 7.04 1 38 0.0098 +1Score > 30 indicates identity U R.KLQLVGVGYK.A
1499   383.5304 1147.5694 1147.5662 2.77 0 18 0.19 +1Score > 42 indicates identity
Score > 23 indicates homology		 U R.DFRPPEPYK.G
1587   592.3469 1182.6792 1182.6721 6.08 1 72 2.2e-005 +1Score > 38 indicates identity U K.QLVGQVAAEIR.D

4 subsets and intersections (21 subset proteins in total)

Score Mass Subset of
RL6_PSE14 76 19506 53.1
50S ribosomal protein L6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplF PE=3 SV=1
8 samesets of RL6_PSE14
RL6_PSESM 76 19266
50S ribosomal protein L6 OS=Pseudomonas syringae pv. tomato GN=rplF PE=3 SV=1
RL6_PSEU2 76 19236
50S ribosomal protein L6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplF PE=3 SV=1
RL6_PSEA7 76 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain PA7) GN=rplF PE=3 SV=1
RL6_PSEA8 76 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplF PE=3 SV=1
RL6_PSEAB 76 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplF PE=3 SV=1
RL6_PSEAE 76 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa GN=rplF PE=3 SV=1
RL6_PSEMY 76 19130
50S ribosomal protein L6 OS=Pseudomonas mendocina (strain ymp) GN=rplF PE=3 SV=1
RL6_PSEU5 76 19167
50S ribosomal protein L6 OS=Pseudomonas stutzeri (strain A1501) GN=rplF PE=3 SV=1
RL6_CHRVO 72 18841 53.1
50S ribosomal protein L6 OS=Chromobacterium violaceum GN=rplF PE=3 SV=1
3 samesets of RL6_CHRVO
RL6_SHELP 72 18777
50S ribosomal protein L6 OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=rplF PE=3 SV=1
RL6_SHESH 72 18751
50S ribosomal protein L6 OS=Shewanella sediminis (strain HAW-EB3) GN=rplF PE=3 SV=1
RL6_SHEWM 72 18852
50S ribosomal protein L6 OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=rplF PE=3 SV=1
RL6_PSEE4 53 19015 53.1
50S ribosomal protein L6 OS=Pseudomonas entomophila (strain L48) GN=rplF PE=3 SV=1
RL6_ACIB3 38 19085 53.1
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplF PE=3 SV=1
6 samesets of RL6_ACIB3
RL6_ACIB5 38 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB0057) GN=rplF PE=3 SV=1
RL6_ACIBS 38 19113
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain SDF) GN=rplF PE=3 SV=1
RL6_ACIBT 38 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=rplF PE=3 SV=2
RL6_ACIBY 38 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AYE) GN=rplF PE=3 SV=1
RL6_AERHH 38 18568
50S ribosomal protein L6 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplF PE=3 SV=1
RL6_AERS4 38 18556
50S ribosomal protein L6 OS=Aeromonas salmonicida (strain A449) GN=rplF PE=3 SV=1

+54

Accession Score Description
1 ODO1_AZOVI 85 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+55

Accession Score Description
1 K2C1_HUMAN 83 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

+56

Accession Score Description
1 KPRS_PSEPK 83 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+57

Accession Score Description
1 SYA_PSEP1 81 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=alaS PE=3 SV=2

+58

Accession Score Description
1 ASPQ_PSEPK 80 Glutaminase-asparaginase OS=Pseudomonas putida (strain KT2440) GN=ansB PE=3 SV=1

+59

Accession Score Description
1 RL7_PSEP1 76 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+60

Accession Score Description
1 YJJK_ECOLI 76 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2
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