MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3754)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
633 380.7209 759.4272 759.4239 4.44 0 41 0.08 +1Score > 42 indicates identity LNSALSR
698 395.7203 789.4260 789.4345 -10.7 0 40 0.075 +1Score > 42 indicates identity TSQVISR
1193 505.7334 1009.4522 1009.4716 -19.2 1 39 0.084 +1Score > 41 indicates identity KYAGVNNNK + 3 Deamidated (NQ)
836 429.7581 857.5016 857.4858 18.5 1 39 0.12 +1Score > 42 indicates identity
Score > 42 indicates homology		 QQLVIEK + Deamidated (NQ)
1173 500.7762 999.5378 999.5349 2.95 0 39 0.066 +1Score > 40 indicates identity
Score > 39 indicates homology		 VANLGNLGDK
665 387.2124 772.4102 772.4079 3.04 1 38 0.21 +1Score > 43 indicates identity IQVQER + Deamidated (NQ)
636 761.4062 760.3989 760.3967 2.99 1 37 0.2 +1Score > 43 indicates identity ETAQAIK + Deamidated (NQ)
1114 486.7993 971.5840 971.5665 18.1 1 37 0.084 +1Score > 39 indicates identity NRIFGLPR
853 433.7474 865.4802 865.4770 3.78 0 37 0.07 +1Score > 38 indicates identity ALHQLQR + Deamidated (NQ)
426 329.7114 657.4082 657.4061 3.25 0 36 0.17 +1Score > 41 indicates identity
Score > 41 indicates homology		 IDVALK
739 407.7546 813.4946 813.5072 -15.5 0 36 0.15 +1Score > 41 indicates identity NIILVSR
1207 508.2632 1014.5118 1014.5094 2.41 1 36 0.14 +1Score > 41 indicates identity
Score > 40 indicates homology		 IADGLNAGER
383 315.7010 629.3874 629.3861 2.20 0 36 0.091 +1Score > 42 indicates identity
Score > 38 indicates homology		 VAVVSR
562 361.6785 721.3424 721.3548 -17.1 0 36 0.18 +1Score > 42 indicates identity
Score > 41 indicates homology		 YPTWR
898 446.2312 890.4478 890.4458 2.35 1 36 0.26 +1Score > 42 indicates identity TGTLTENR
1071 479.2991 956.5836 956.5728 11.3 1 35 0.12 +1Score > 39 indicates identity KMLPNLLK + Deamidated (NQ)
661 386.6972 771.3798 771.3875 -9.94 0 35 0.16 +1Score > 40 indicates identity LNGTGGPR + Deamidated (NQ)
1038 474.2391 946.4636 946.4607 3.11 0 35 0.061 +1Score > 42 indicates identity
Score > 35 indicates homology		 AQVQAQTAK + 3 Deamidated (NQ)
1062 478.7837 955.5528 955.5451 8.15 1 35 0.079 +1Score > 40 indicates identity
Score > 36 indicates homology		 VAEQLAAVR
987 928.4182 927.4109 927.3942 18.0 1 35 0.11 +1Score > 38 indicates identity EFMMQSR
742 408.2360 814.4574 814.4548 3.19 1 35 0.35 +1Score > 43 indicates identity EIIIDGR
878 440.2534 878.4922 878.4861 6.96 0 35 0.13 +1Score > 38 indicates identity YILNSIR + Deamidated (NQ)
1699 614.8386 1227.6626 1227.6823 -16.0 0 35 0.2 +1Score > 40 indicates identity TNQALGQALLAK + Deamidated (NQ)
434 333.6833 665.3520 665.3497 3.60 0 35 0.14 +1Score > 39 indicates identity AGYSLR
785 841.5178 840.5105 840.5069 4.33 1 35 0.068 +1Score > 35 indicates identity KIDQIPK
1027 472.7448 943.4750 943.4909 -16.8 1 35 0.15 +1Score > 41 indicates identity
Score > 39 indicates homology		 KAINPNMR + Deamidated (NQ)
1088 962.5459 961.5386 961.5457 -7.39 1 34 0.14 +1Score > 39 indicates identity
Score > 39 indicates homology		 QVGYALRR
1814 640.3575 1278.7004 1278.6932 5.70 1 34 0.07 +1Score > 38 indicates identity
Score > 35 indicates homology		 LQAGLHQAIEAK + Deamidated (NQ)
552 358.2265 714.4384 714.4388 -0.51 0 34 0.42 +1Score > 43 indicates identity LASVGIR
468 675.3719 674.3646 674.3599 7.04 1 34 0.37 +1Score > 42 indicates identity AAESVAK
408 643.4178 642.4105 642.4177 -11.1 1 34 0.3 +1Score > 41 indicates identity ARVVAK
1177 501.2891 1000.5636 1000.5665 -2.89 1 34 0.3 +1Score > 41 indicates identity AGSGGGGVALKK
253 516.2925 515.2852 515.2816 7.09 0 34 0.23 +1Score > 41 indicates identity
Score > 40 indicates homology		 AGAAAR
1516 578.8235 1155.6324 1155.6248 6.64 0 34 0.22 +1Score > 40 indicates identity SPATVLADLNR
706 398.2234 794.4322 794.4286 4.55 0 34 0.2 +1Score > 39 indicates identity AVSSYLR
1136 494.2794 986.5442 986.5509 -6.73 0 33 0.37 +1Score > 42 indicates identity VVATAAAATGR
1774 631.3566 1260.6986 1260.6965 1.68 0 33 0.2 +1Score > 39 indicates identity QFILSQVALLE + Deamidated (NQ)
1180 502.2591 1002.5036 1002.5233 -19.6 0 33 0.38 +1Score > 43 indicates identity
Score > 42 indicates homology		 IAIITNNNK + 3 Deamidated (NQ)
666 387.2130 772.4114 772.4079 4.60 1 33 0.17 +1Score > 44 indicates identity
Score > 38 indicates homology		 IQVQER + Deamidated (NQ)
462 673.4276 672.4203 672.4170 4.91 0 33 0.27 +1Score > 40 indicates identity AVSVAVK
1503 576.7717 1151.5288 1151.5260 2.43 1 33 0.17 +1Score > 41 indicates identity
Score > 38 indicates homology		 NYWNGRWR + Deamidated (NQ)
3896 837.0781 2508.2125 2508.1737 15.4 1 33 0.18 +1Score > 38 indicates identity
Score > 38 indicates homology		 RMGQSGCDIVSVDWTVDLLDAR + Oxidation (M)
905 894.4702 893.4629 893.4607 2.53 1 33 0.41 +1Score > 41 indicates identity GISEGLYR
271 544.3486 543.3413 543.3380 6.04 0 33 0.66 +1Score > 44 indicates identity
Score > 43 indicates homology		 AGAVVK
583 365.2306 728.4466 728.4432 4.70 1 33 0.35 +1Score > 42 indicates identity
Score > 41 indicates homology		 VAIEAVK
514 700.4389 699.4316 699.4279 5.32 0 33 0.36 +1Score > 41 indicates identity QAALGLK
513 350.7227 699.4308 699.4391 -11.8 1 32 0.21 +1Score > 41 indicates identity
Score > 38 indicates homology		 AAKAAIR
812 425.7248 849.4350 849.4344 0.71 0 32 0.082 +1Score > 42 indicates identity
Score > 34 indicates homology		 YNDAVIR
1188 505.2543 1008.4940 1008.5128 -18.6 1 32 0.5 +1Score > 42 indicates identity QTLDYIEK
737 407.7446 813.4746 813.4821 -9.11 1 32 0.44 +1Score > 41 indicates identity QAKGIAAR
1402 371.5470 1111.6192 1111.6210 -1.65 1 32 0.23 +1Score > 38 indicates identity AINANGGRALR
364 311.6700 621.3254 621.3235 3.21 0 32 0.16 +1Score > 40 indicates identity
Score > 37 indicates homology		 AGGVYR
1773 631.3564 1260.6982 1260.6747 18.7 1 32 0.27 +1Score > 39 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
1022 471.7733 941.5320 941.5181 14.8 1 32 0.3 +1Score > 39 indicates identity LAEAAKPNK + Deamidated (NQ)
870 437.7532 873.4918 873.4920 -0.13 1 32 0.48 +1Score > 41 indicates identity AELTIATR
930 452.2491 902.4836 902.4934 -10.8 1 32 0.58 +1Score > 42 indicates identity TARDAITR
1024 471.7929 941.5712 941.5545 17.8 1 32 0.26 +1Score > 38 indicates identity IIEALNIR + Deamidated (NQ)
809 424.7366 847.4586 847.4585 0.13 1 32 0.26 +1Score > 41 indicates identity
Score > 38 indicates homology		 LNKQMAK + Oxidation (M)
1371 547.7865 1093.5584 1093.5516 6.26 0 32 0.14 +1Score > 41 indicates identity
Score > 36 indicates homology		 AATNGAPGVAHK + Deamidated (NQ)
1629 601.3389 1200.6632 1200.6437 16.2 0 32 0.16 +1Score > 40 indicates identity
Score > 36 indicates homology		 MLNVVWNAVR
466 338.1874 674.3602 674.3711 -16.1 0 31 0.2 +1Score > 43 indicates identity
Score > 37 indicates homology		 TIGSAAR
1696 614.8384 1227.6622 1227.6823 -16.3 0 31 0.41 +1Score > 40 indicates identity IDVGGSAAVLGAAK
1069 479.2766 956.5386 956.5291 10.0 1 31 0.48 +1Score > 41 indicates identity IGNAIAELR + Deamidated (NQ)
409 322.7032 643.3918 643.3904 2.19 1 31 0.68 +1Score > 43 indicates identity
Score > 42 indicates homology		 AIEIAK
632 760.4335 759.4262 759.4239 3.09 0 31 0.22 +1Score > 42 indicates identity
Score > 37 indicates homology		 LNSALSR
1768 629.8569 1257.6992 1257.6816 14.0 0 31 0.37 +1Score > 39 indicates identity LVITQGALSQVE + Deamidated (NQ)
1976 453.5817 1357.7233 1357.7201 2.33 1 31 0.16 +1Score > 40 indicates identity
Score > 36 indicates homology		 QTALSIEQQLAR + Deamidated (NQ)
805 424.6998 847.3850 847.3937 -10.2 1 31 0.1 +1Score > 41 indicates identity
Score > 34 indicates homology		 DDTWRR
491 689.3893 688.3820 688.3755 9.43 1 31 0.39 +1Score > 43 indicates identity
Score > 40 indicates homology		 DDKAIK
2380 520.6170 1558.8292 1558.8144 9.50 0 31 0.082 +1Score > 39 indicates identity
Score > 33 indicates homology		 LSFLPSHFIQVNR + 2 Deamidated (NQ)
406 643.4160 642.4087 642.4177 -13.9 1 31 0.42 +1Score > 41 indicates identity
Score > 40 indicates homology		 GRAVLK
803 423.7284 845.4422 845.4395 3.20 1 31 0.22 +1Score > 42 indicates identity
Score > 37 indicates homology		 IPGFQER
1246 516.2626 1030.5106 1030.5043 6.14 0 31 0.75 +1Score > 42 indicates identity LTSNEPSQR
1691 613.8480 1225.6814 1225.6779 2.92 1 31 0.31 +1Score > 38 indicates identity QLVAELQGNVR
921 450.2879 898.5612 898.5488 13.9 0 31 0.19 +1Score > 37 indicates identity
Score > 36 indicates homology		 AVLDQLLK
1245 516.2618 1030.5090 1030.5043 4.59 0 31 0.092 +1Score > 42 indicates identity
Score > 33 indicates homology		 LTSNEPSQR
1698 614.8386 1227.6626 1227.6823 -16.0 0 30 0.38 +1Score > 40 indicates identity
Score > 39 indicates homology		 IDVGGSAAVLGAAK
621 379.2336 756.4526 756.4494 4.33 1 30 0.7 +1Score > 41 indicates identity VAEVALR
1775 631.3566 1260.6986 1260.6747 19.0 1 30 0.39 +1Score > 39 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
1769 629.8570 1257.6994 1257.6816 14.2 0 30 0.15 +1Score > 39 indicates identity
Score > 35 indicates homology		 LVITQGALSQVE + Deamidated (NQ)
668 775.4343 774.4270 774.4236 4.46 0 30 0.77 +1Score > 43 indicates identity
Score > 42 indicates homology		 GNSLTGVK
2057 350.7106 1398.8133 1398.8194 -4.39 1 30 0.16 +1Score > 35 indicates identity DLTLLRTDLALR
767 415.7420 829.4694 829.4545 18.0 1 30 0.22 +1Score > 43 indicates identity
Score > 36 indicates homology		 KIIDNVE
1244 516.2615 1030.5084 1030.4931 14.9 0 30 0.44 +1Score > 41 indicates identity
Score > 39 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
283 559.3223 558.3150 558.3238 -15.7 1 30 0.87 +1Score > 45 indicates identity
Score > 42 indicates homology		 KAGGAR
1255 1035.5878 1034.5805 1034.5760 4.34 1 30 0.39 +1Score > 38 indicates identity IFGNLDTKK
1416 373.8547 1118.5423 1118.5641 -19.6 0 30 0.49 +1Score > 42 indicates identity
Score > 39 indicates homology		 MIDNITGDLK
1109 486.7666 971.5186 971.5148 3.93 0 30 0.082 +1Score > 42 indicates identity
Score > 31 indicates homology		 SAGLQVQNR
769 416.2466 830.4786 830.4749 4.50 1 30 0.64 +1Score > 40 indicates identity TQVELLK + Deamidated (NQ)
693 395.7018 789.3890 789.3981 -11.4 0 30 0.39 +1Score > 43 indicates identity
Score > 38 indicates homology		 SDNVLSR
226 487.3017 486.2944 486.2914 6.19 0 30 0.87 +1Score > 42 indicates identity
Score > 42 indicates homology		 AVAAR
1419 560.7935 1119.5724 1119.5924 -17.8 1 30 0.13 +1Score > 42 indicates identity
Score > 33 indicates homology		 QFLNEILSR + Deamidated (NQ)
422 328.6912 655.3678 655.3765 -13.3 0 29 0.18 +1Score > 37 indicates identity
Score > 35 indicates homology		 IDRPR
630 380.2544 758.4942 758.4902 5.37 0 29 0.061 +1Score > 30 indicates identity VISILSK
549 357.2520 712.4894 712.4847 6.69 0 29 0.16 +1Score > 34 indicates identity NIIILK
431 331.6970 661.3794 661.3833 -5.76 0 29 0.46 +1Score > 38 indicates identity ISLMAK
259 519.3160 518.3087 518.3064 4.49 0 29 0.19 +1Score > 35 indicates identity ATISK
550 357.2520 712.4894 712.4847 6.69 0 29 0.17 +1Score > 34 indicates identity NIIILK
427 329.7114 657.4082 657.4061 3.25 1 29 0.097 +1Score > 41 indicates identity
Score > 31 indicates homology		 IEIVGK
1376 549.3169 1096.6192 1096.6063 11.8 1 29 0.25 +1Score > 37 indicates identity
Score > 36 indicates homology		 LPDIGPKMAR
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