MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1194 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 3 | EFTU_CARRP | 163 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1113 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 941 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSESM | 569 | ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 882 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 169 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 206 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 450 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 70 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 115 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 402 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | RL1_PSEE4 |
402 | 24208 | 14 (9) | 7 (4) | 0.58 | |
| 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 | |||||||
| 2 samesets of RL1_PSEE4 | |||||||
| RL1_PSEP1 |
402 | 24236 | 14 (9) | 7 (4) | 0.58 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplA PE=3 SV=1 | |||||||
| RL1_PSEPK |
402 | 24236 | 14 (9) | 7 (4) | 0.58 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain KT2440) GN=rplA PE=3 SV=1 | |||||||
14 peptide matches (8 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 264 |
531.2908 | 530.2835 | 530.2813 | 4.29 | 0 | 19 | 3.2 | 7Score > 46 indicates identity Score > 36 indicates homology |
K.AGQVR.Y + Deamidated (NQ) | ||
| 1230 |
342.5305 | 1024.5697 | 1024.5665 | 3.05 | 0 | 31 | 0.2 | 1Score > 37 indicates identity Score > 37 indicates homology |
U | K.NGIIHTSVGK.V | |
| 1313 |
356.5344 | 1066.5814 | 1066.5771 | 3.99 | 0 | 29 | 0.12 | 1Score > 38 indicates identity Score > 32 indicates homology |
U | R.SATVLPHGTGK.T | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_4_20250714120925.rawScore > 32 indicates identity Score > 26 indicates homology |
|||||||||||
| 1683 |
2 |
611.8718 | 1221.7290 | 1221.7194 | 7.92 | 0 | 67 | 4.4e-006 | 1Score > 32 indicates identity Score > 26 indicates homology |
U | R.VVGQLGQVLGPR.G |
| 13.3 | 1 | 9 | 2.5 | 2 | VVMLRAQLHR | ||||||
| -1.25 | 1 | 9 | 2.9 | 3 | GLNVLQPARVR | ||||||
| -10.4 | 1 | 8 | 3.8 | 4 | VQRLRPLGQR | ||||||
| -10.4 | 1 | 8 | 3.8 | 4 | VRQLRPLGQR | ||||||
| 17.1 | 1 | 4 | 9.8 | 6 | DGRLIVDLPPK | ||||||
| 7.95 | 1 | 3 | 11 | 7 | VQILNIHTKR + Deamidated (NQ) | ||||||
| 7.93 | 1 | 2 | 14 | 8 | VVIDRSHVIGK | ||||||
| 1767 |
629.3590 | 1256.7034 | 1256.6976 | 4.62 | 0 | 61 | 5.3e-005 | 1Score > 39 indicates identity Score > 30 indicates homology |
U | K.VGTVTPDVATAVK.N | |
| 2851 |
3 |
897.4639 | 1792.9132 | 1792.8996 | 7.63 | 1 | 45 | 0.00034 | 1Score > 40 indicates identity Score > 23 indicates homology |
U | K.FVESYDIAVNLGVDPR.K |
| 3004 |
1 |
943.5013 | 1884.9880 | 1884.9694 | 9.91 | 1 | 122 | 5.2e-011 | 1Score > 39 indicates identity Score > 32 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V |
| 3005 |
629.3395 | 1884.9967 | 1884.9694 | 14.5 | 1 | 58 | 3.6e-005 | 1Score > 38 indicates identity Score > 26 indicates homology |
U | R.VAVFTQGPAAEAALAAGADR.V | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL1_PSEPG |
370 | 24180 | 5.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain GB-1) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEPW |
362 | 24112 | 5.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas putida (strain W619) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEPW | |||||
| RL1_PSESM |
362 | 24163 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. tomato GN=rplA PE=3 SV=1 | |||||
| RL1_PSE14 |
331 | 24120 | 5.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplA PE=3 SV=1 | |||||
| 2 samesets of RL1_PSE14 | |||||
| RL1_PSEF5 |
331 | 24047 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEU2 |
331 | 24176 | |||
| 50S ribosomal protein L1 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEFS |
258 | 24061 | 5.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain SBW25) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEFS | |||||
| RL1_PSEPF |
258 | 24193 | |||
| 50S ribosomal protein L1 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplA PE=3 SV=1 | |||||
| RL1_AZOVD |
228 | 24230 | 5.1 | ||
| 50S ribosomal protein L1 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplA PE=3 SV=1 | |||||
| RL1_PSEMY |
163 | 23957 | 5.1 | ||
| 50S ribosomal protein L1 OS=Pseudomonas mendocina (strain ymp) GN=rplA PE=3 SV=1 | |||||
| 1 sameset of RL1_PSEMY | |||||
| RL1_PSEU5 |
163 | 24129 | |||
| 50S ribosomal protein L1 OS=Pseudomonas stutzeri (strain A1501) GN=rplA PE=3 SV=1 | |||||
| RL1_ACTP2 |
130 | 23932 | 5.1 | ||
| 50S ribosomal protein L1 OS=Actinobacillus pleuropneumoniae serotype 5b (strain L20) GN=rplA PE=3 SV=1 | |||||
| 58 samesets of RL1_ACTP2 |
|||||
| RL1_COXB1 |
61 | 24793 | 5.1 | ||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain CbuK_Q154) GN=rplA PE=3 SV=1 | |||||
| 4 samesets of RL1_COXB1 | |||||
| RL1_COXB2 |
61 | 24793 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain CbuG_Q212) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBN |
61 | 24793 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain Dugway 5J108-111) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBR |
61 | 24823 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=rplA PE=3 SV=1 | |||||
| RL1_COXBU |
61 | 24823 | |||
| 50S ribosomal protein L1 OS=Coxiella burnetii GN=rplA PE=3 SV=1 | |||||
| RL1_ACIAC |
61 | 23909 | 5.1 | ||
| 50S ribosomal protein L1 OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rplA PE=3 SV=1 | |||||
| 46 samesets of RL1_ACIAC |
|||||
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 364 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 308 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 290 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 78 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEP1 | 287 | 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSE14 | 192 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 280 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
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