MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 182)

Page: Previous 1 2 3 4 5 6 7 8 9 10 … 19 Next 

+41

Accession Score Description
1 RL25_PSEPK 112 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+42

Accession Score Description
1 DNAK_PSEPK 110 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+43

Accession Score Description
1 ETFB_PSEAE 109 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+44

Accession Score Description
1 DLDH2_PSEPU 107 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+45

Accession Score Description
1 RS11_AZOVD 106 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+46

Accession Score Description
1 GUAA_CELJU 104 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+47

Accession Score Description
1 RL22_PSE14 103 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

-48

Accession Score Description
1 RL20_PSEE4 96 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
Score Mass Matches Sequences emPAI
48.1 RL20_PSEE4 96 13272 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
8 samesets of RL20_PSEE4
RL20_PSEF5 96 13287 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplT PE=3 SV=1
RL20_PSEFL 96 13287 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas fluorescens GN=rplT PE=3 SV=1
RL20_PSEMY 96 13241 10 (5) 7 (4) 0.94
50S ribosomal protein L20 OS=Pseudomonas mendocina (strain ymp) GN=rplT PE=3 SV=1
RL20_PSEP1 96 13272 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplT PE=3 SV=1
RL20_PSEPG 96 13272 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain GB-1) GN=rplT PE=3 SV=1
RL20_PSEPK 96 13272 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
RL20_PSEPW 96 13272 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain W619) GN=rplT PE=3 SV=1
RL20_PSEU5 96 13284 10 (5) 7 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas stutzeri (strain A1501) GN=rplT PE=3 SV=1

-10 peptide matches (7 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
281   558.3632 557.3559 557.3537 4.02 0 22 1.8 +8Score > 40 indicates identity
Score > 37 indicates homology		 K.QAVIK.A
348 +1 307.7165 613.4184 613.4163 3.54 0 36 0.05 +1Score > 36 indicates identity U R.LIAGLK.K
485   343.6678 685.3210 685.3184 3.92 0 3 2.5 +6Score > 37 indicates identity
Score > 19 indicates homology		 K.GYYGAR.S
582   365.2259 728.4372 728.4333 5.39 0 16 1.1 +2Score > 41 indicates identity
Score > 29 indicates homology		 R.ALWIAR.I
762   414.7047 827.3948 827.3926 2.74 0 32 0.047 +1Score > 38 indicates identity
Score > 31 indicates homology		 U K.AGQYAYR.D
962 +1 460.2687 918.5228 918.5174 5.89 1 35 0.0042 +1Score > 39 indicates identity
Score > 24 indicates homology		 U K.AAFAAIVEK.A
1322 +1 536.3092 1070.6038 1070.5972 6.25 1 65 0.0002 +1Score > 40 indicates identity U K.VLADLAVNEK.A

7 subsets and intersections (278 subset proteins in total)

Score Mass Subset of
RL20_PSE14 96 13306 48.1
50S ribosomal protein L20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplT PE=3 SV=1
4 samesets of RL20_PSE14
RL20_PSEPF 96 13306
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplT PE=3 SV=1
RL20_PSESM 96 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. tomato GN=rplT PE=3 SV=1
RL20_PSESY 96 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae GN=rplT PE=3 SV=1
RL20_PSEU2 96 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplT PE=3 SV=1
RL20_PSEA7 88 13357 48.1
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain PA7) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEA7
RL20_PSEA8 88 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplT PE=3 SV=1
RL20_PSEAB 88 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplT PE=3 SV=1
RL20_PSEAE 88 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa GN=rplT PE=3 SV=1
RL20_AZOVD 43 13385 48.1
50S ribosomal protein L20 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplT PE=3 SV=1
1 sameset of RL20_AZOVD
RL20_AZOVI 43 13429
50S ribosomal protein L20 OS=Azotobacter vinelandii GN=rplT PE=3 SV=1
RL20_POLNS 40 13738 48.1
50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
2 samesets of RL20_POLNS
RL20_POLSQ 40 13742
50S ribosomal protein L20 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rplT PE=3 SV=1
RL20_PROMH 40 13449
50S ribosomal protein L20 OS=Proteus mirabilis (strain HI4320) GN=rplT PE=3 SV=1
ARLY_LACRD 36 52058 48.1
Argininosuccinate lyase OS=Lactobacillus reuteri (strain DSM 20016) GN=argH PE=3 SV=1
+28 samesets of ARLY_LACRD
RL20_HAHCH 35 13431 48.1
50S ribosomal protein L20 OS=Hahella chejuensis (strain KCTC 2396) GN=rplT PE=3 SV=1
3 samesets of RL20_HAHCH
RL20_MARAV 35 13234
50S ribosomal protein L20 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rplT PE=3 SV=1
RL20_SACD2 35 13355
50S ribosomal protein L20 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplT PE=3 SV=1
RL20_TERTT 35 13373
50S ribosomal protein L20 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplT PE=3 SV=1
RL20_ACIAC 32 13531 48.1
50S ribosomal protein L20 OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rplT PE=3 SV=1
+230 samesets of RL20_ACIAC

+49

Accession Score Description
1 YEAG_ECOLI 95 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+50

Accession Score Description
1 PHAA_PSEOL 94 Poly(3-hydroxyalkanoate) polymerase 1 OS=Pseudomonas oleovorans GN=phaA PE=3 SV=1
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