MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 182)

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+41

Accession Score Description
1 RL25_PSEPK 112 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+42

Accession Score Description
1 DNAK_PSEPK 110 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+43

Accession Score Description
1 ETFB_PSEAE 109 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+44

Accession Score Description
1 DLDH2_PSEPU 107 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

-45

Accession Score Description
1 RS11_AZOVD 106 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1
Score Mass Matches Sequences emPAI
45.1 RS11_AZOVD 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1
17 samesets of RS11_AZOVD
RS11_PSE14 106 13721 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsK PE=3 SV=1
RS11_PSEA7 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsK PE=3 SV=1
RS11_PSEA8 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsK PE=3 SV=1
RS11_PSEAB 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsK PE=3 SV=1
RS11_PSEAE 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas aeruginosa GN=rpsK PE=3 SV=1
RS11_PSEE4 106 13765 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1
RS11_PSEF5 106 13735 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsK PE=3 SV=1
RS11_PSEFS 106 13721 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsK PE=3 SV=1
RS11_PSEMY 106 13753 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas mendocina (strain ymp) GN=rpsK PE=3 SV=1
RS11_PSEP1 106 13765 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsK PE=3 SV=1
RS11_PSEPF 106 13721 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsK PE=3 SV=1
RS11_PSEPG 106 13779 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas putida (strain GB-1) GN=rpsK PE=3 SV=1
RS11_PSEPK 106 13765 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1
RS11_PSEPW 106 13765 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas putida (strain W619) GN=rpsK PE=3 SV=1
RS11_PSESM 106 13721 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas syringae pv. tomato GN=rpsK PE=3 SV=1
RS11_PSEU2 106 13721 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsK PE=3 SV=1
RS11_PSEU5 106 13749 3 (2) 2 (2) 0.38
30S ribosomal protein S11 OS=Pseudomonas stutzeri (strain A1501) GN=rpsK PE=3 SV=1

-3 peptide matches (2 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1425 +1 560.8076 1119.6006 1119.5924 7.37 1 56 0.0018 +1Score > 41 indicates identity U R.AGQAALEYGLK.N
2464   798.3846 1594.7546 1594.7488 3.65 0 91 4.7e-007 +1Score > 40 indicates identity U R.QGNALSWATSGGSGFR.G

1 subset or intersection (32 subset proteins in total)

Score Mass Subset of
RS11_AERHH 91 13883 45.1
30S ribosomal protein S11 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rpsK PE=3 SV=1
+31 samesets of RS11_AERHH

+46

Accession Score Description
1 GUAA_CELJU 104 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+47

Accession Score Description
1 RL22_PSE14 103 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+48

Accession Score Description
1 RL20_PSEE4 96 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

+49

Accession Score Description
1 YEAG_ECOLI 95 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+50

Accession Score Description
1 PHAA_PSEOL 94 Poly(3-hydroxyalkanoate) polymerase 1 OS=Pseudomonas oleovorans GN=phaA PE=3 SV=1
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