MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 182)

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+41

Accession Score Description
1 RL25_PSEPK 112 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+42

Accession Score Description
1 DNAK_PSEPK 110 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+43

Accession Score Description
1 ETFB_PSEAE 109 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

-44

Accession Score Description
1 DLDH2_PSEPU 107 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4
Score Mass Matches Sequences emPAI
44.1 DLDH2_PSEPU 107 50093 18 (4) 10 (3) 0.20
Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

-18 peptide matches (13 non-duplicate, 5 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
229 +2 487.3270 486.3197 486.3166 6.48 0 30 1.6 +3Score > 46 indicates identity
Score > 44 indicates homology		 K.AGIVK.N
350   308.2142 614.4138 614.4115 3.77 1 10 1.4 +7Score > 38 indicates identity
Score > 24 indicates homology		 R.KAGIVK.N
368 +1 312.1742 622.3338 622.3326 1.96 0 14 0.22 +1Score > 41 indicates identity
Score > 20 indicates homology		 K.ITFDK.L
493   689.3964 688.3891 688.3755 19.7 1 20 3.4 +9Score > 43 indicates identity
Score > 38 indicates homology		 K.TEQALK.A
513   350.7227 699.4308 699.4279 4.21 0 24 1.4 +3Score > 41 indicates identity
Score > 38 indicates homology		 K.AAQLGLK.T
514   700.4389 699.4316 699.4279 5.32 0 30 0.61 +2Score > 41 indicates identity K.AAQLGLK.T
573 +2 364.2464 726.4782 726.4752 4.19 0 35 0.062 +1Score > 36 indicates identity U K.LIVAVGR.R
959   460.2507 918.4868 918.4811 6.30 0 34 0.25 +1Score > 42 indicates identity
Score > 41 indicates homology		 U K.ALLDSSWK.Y
1426   560.8276 1119.6406 1119.6288 10.6 0 37 0.0019 +1Score > 36 indicates identity
Score > 23 indicates homology		 U K.NLTGGVATLFK.A
1430   561.3224 1120.6302 1120.6128 15.5 0 7 1.3 +2Score > 38 indicates identity
Score > 21 indicates homology		 U K.NLTGGVATLFK.A + Deamidated (NQ)
1544   584.8093 1167.6040 1167.5996 3.78 0 55 0.0002 +1Score > 40 indicates identity
Score > 30 indicates homology		 U K.ANGVTSIQGHGK.L
1545   390.2088 1167.6046 1167.5996 4.22 0 20 0.056 +1Score > 40 indicates identity
Score > 20 indicates homology		 U K.ANGVTSIQGHGK.L
2974   934.0599 1866.1052 1866.0727 17.4 1 71 3.2e-006 +1Score > 28 indicates identity U R.LGVIGAGVIGLELGSVWAR.L

1 subset or intersection (3 subset proteins in total)

Score Mass Subset of
DLDH2_PSEAE 71 50362 44.1
Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1
2 samesets of DLDH2_PSEAE
DLDH_PSEFL 71 50348
Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3
DLDH_AZOVI 71 49707
Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1

+45

Accession Score Description
1 RS11_AZOVD 106 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+46

Accession Score Description
1 GUAA_CELJU 104 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+47

Accession Score Description
1 RL22_PSE14 103 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+48

Accession Score Description
1 RL20_PSEE4 96 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1

+49

Accession Score Description
1 YEAG_ECOLI 95 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+50

Accession Score Description
1 PHAA_PSEOL 94 Poly(3-hydroxyalkanoate) polymerase 1 OS=Pseudomonas oleovorans GN=phaA PE=3 SV=1
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