MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 182)

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+31

Accession Score Description
1 RISB_PSEPG 138 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+32

Accession Score Description
1 ARPC_PSEPU 136 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+33

Accession Score Description
1 PNP_PSEPG 134 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1

-34

Accession Score Description
1 SAHH_PSEP1 130 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
Score Mass Matches Sequences emPAI
34.1 SAHH_PSEP1 130 51870 6 (4) 5 (4) 0.19
Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1
1 sameset of SAHH_PSEP1
SAHH_PSEPF 130 51842 5 (4) 4 (4) 0.19
Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1

+6 peptide matches (6 non-duplicate, 0 duplicate)

6 subsets and intersections (16 subset proteins in total)

Score Mass Subset of
SAHH_PSEPG 126 51934 34.1
Adenosylhomocysteinase OS=Pseudomonas putida (strain GB-1) GN=ahcY PE=3 SV=1
SAHH_PSE14 95 51802 34.1
Adenosylhomocysteinase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ahcY PE=3 SV=1
1 sameset of SAHH_PSE14
SAHH_PSEU2 95 51844
Adenosylhomocysteinase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ahcY PE=3 SV=1
SAHH_PSEF5 71 51812 34.1
Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ahcY PE=3 SV=1
2 samesets of SAHH_PSEF5
SAHH_PSEMY 71 51753
Adenosylhomocysteinase OS=Pseudomonas mendocina (strain ymp) GN=ahcY PE=3 SV=1
SAHH_PSEFS 71 51668
Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain SBW25) GN=ahcY PE=3 SV=1
SAHH_HAHCH 67 51771 34.1
Adenosylhomocysteinase OS=Hahella chejuensis (strain KCTC 2396) GN=ahcY PE=3 SV=1
7 samesets of SAHH_HAHCH
SAHH_PSEAB 67 51849
Adenosylhomocysteinase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ahcY PE=3 SV=1
SAHH_PSEAE 67 51823
Adenosylhomocysteinase OS=Pseudomonas aeruginosa GN=ahcY PE=3 SV=1
SAHH_PSEPW 67 51814
Adenosylhomocysteinase OS=Pseudomonas putida (strain W619) GN=ahcY PE=3 SV=1
SAHH_PSESM 67 51772
Adenosylhomocysteinase OS=Pseudomonas syringae pv. tomato GN=ahcY PE=3 SV=1
SAHH_PSEE4 67 51821
Adenosylhomocysteinase OS=Pseudomonas entomophila (strain L48) GN=ahcY PE=3 SV=1
SAHH_PSEU5 67 51481
Adenosylhomocysteinase OS=Pseudomonas stutzeri (strain A1501) GN=ahcY PE=3 SV=1
SAHH_PSEA8 67 51823
Adenosylhomocysteinase OS=Pseudomonas aeruginosa (strain LESB58) GN=ahcY PE=3 SV=1
SAHH_SYNSC 50 52404 34.1
Adenosylhomocysteinase OS=Synechococcus sp. (strain CC9605) GN=ahcY PE=3 SV=1
SAHH_ACIAD 44 51793 34.1
Adenosylhomocysteinase OS=Acinetobacter sp. (strain ADP1) GN=ahcY PE=3 SV=1

+35

Accession Score Description
1 ATPD_PSEPG 129 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+36

Accession Score Description
1 ATPF_PSEPK 127 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+37

Accession Score Description
1 ODP2_PSEAE 124 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+38

Accession Score Description
1 LEU1_PSEP1 124 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+39

Accession Score Description
1 RS16_PSEP1 116 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+40

Accession Score Description
1 RL3_PSEE4 112 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
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