MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 182)


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+31

Accession Score Description
1 RISB_PSEPG 138 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+32

Accession Score Description
1 ARPC_PSEPU 136 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

-33

Accession Score Description
1 PNP_PSEPG 134 Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1
Score Mass Matches Sequences emPAI
33.1 PNP_PSEPG 134 75207 10 (5) 8 (4) 0.16
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain GB-1) GN=pnp PE=3 SV=1
4 samesets of PNP_PSEPG
PNP_PSEPK 134 75192 10 (5) 8 (4) 0.16
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain KT2440) GN=pnp PE=3 SV=1
PNP_PSEPU 134 75166 10 (5) 8 (4) 0.16
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida GN=pnp PE=3 SV=1
PNP_PSEP1 134 75139 10 (5) 8 (4) 0.16
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pnp PE=3 SV=1
PNP_PSEE4 134 75140 10 (5) 8 (4) 0.16
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas entomophila (strain L48) GN=pnp PE=3 SV=1

-10 peptide matches (9 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
648   383.2201 764.4256 764.4221 4.62 0 23 0.087 +1Score > 39 indicates identity
Score > 25 indicates homology
K.IPGGFFK.R
1776   631.8134 1261.6122 1261.6038 6.73 1 39 0.0076 +1Score > 42 indicates identity
Score > 30 indicates homology
U K.ASIDIEDDGSIK.I
2096   707.3744 1412.7342 1412.7082 18.4 0 19 1.7 +4Score > 40 indicates identity
Score > 34 indicates homology
U K.DGLVHISMLSDAR.V
2162   726.3790 1450.7434 1450.7245 13.1 1 2 1.2 +3Score > 41 indicates identity
Score > 15 indicates homology
U K.GFFPLSVHYQEK.T
2227   743.9203 1485.8260 1485.8151 7.36 1 78 5.6e-006 +1Score > 38 indicates identity U R.GETQALVVATLGTAR.D
2230   745.4352 1488.8558 1488.8341 14.6 0 16 0.09 +1Score > 34 indicates identity
Score > 18 indicates homology
R.IVDFGAFVNILPGK.D
2330   767.9773 1533.9400 1533.9243 10.3 1 27 0.023 +1Score > 25 indicates identity
Score > 24 indicates homology
U K.TVRPLNIEVGVLPK.T
2332 +1 512.3214 1533.9424 1533.9243 11.8 1 33 0.0099 +1Score > 25 indicates identity U K.TVRPLNIEVGVLPK.T
2646   836.4253 1670.8360 1670.8264 5.76 1 73 2.1e-006 +1Score > 40 indicates identity
Score > 28 indicates homology
U K.TFQFGQSTVTLETGR.I

5 subsets and intersections (17 subset proteins in total)

Score Mass Subset of
PNP_AZOVD 126 75912 33.1
Polyribonucleotide nucleotidyltransferase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=pnp PE=3 SV=1
PNP_PSE14 89 75121 33.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=pnp PE=3 SV=1
6 samesets of PNP_PSE14
PNP_PSEFS 89 75117
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain SBW25) GN=pnp PE=3 SV=1
PNP_PSEPF 89 75026
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf0-1) GN=pnp PE=3 SV=1
PNP_PSEPW 89 75250
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas putida (strain W619) GN=pnp PE=3 SV=1
PNP_PSESM 89 75108
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. tomato GN=pnp PE=3 SV=1
PNP_PSEU2 89 75123
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=pnp PE=3 SV=1
PNP_PSEF5 89 75343
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=pnp PE=3 SV=1
PNP_PSEMY 83 75677 33.1
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas mendocina (strain ymp) GN=pnp PE=3 SV=2
1 sameset of PNP_PSEMY
PNP_PSEU5 83 75420
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas stutzeri (strain A1501) GN=pnp PE=3 SV=1
PNP_POLNS 78 77289 33.1
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter necessarius (strain STIR1) GN=pnp PE=3 SV=1
5 samesets of PNP_POLNS
PNP_PSEAB 78 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=pnp PE=3 SV=1
PNP_PSEAE 78 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa GN=pnp PE=3 SV=1
PNP_PSEA7 78 75691
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain PA7) GN=pnp PE=3 SV=1
PNP_PSEA8 78 75634
Polyribonucleotide nucleotidyltransferase OS=Pseudomonas aeruginosa (strain LESB58) GN=pnp PE=3 SV=1
PNP_POLSQ 78 77293
Polyribonucleotide nucleotidyltransferase OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=pnp PE=3 SV=1
PNP_TERTT 27 76334 33.1
Polyribonucleotide nucleotidyltransferase OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=pnp PE=3 SV=1

+34

Accession Score Description
1 SAHH_PSEP1 130 Adenosylhomocysteinase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ahcY PE=3 SV=1

+35

Accession Score Description
1 ATPD_PSEPG 129 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+36

Accession Score Description
1 ATPF_PSEPK 127 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+37

Accession Score Description
1 ODP2_PSEAE 124 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+38

Accession Score Description
1 LEU1_PSEP1 124 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+39

Accession Score Description
1 RS16_PSEP1 116 30S ribosomal protein S16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsP PE=3 SV=1

+40

Accession Score Description
1 RL3_PSEE4 112 50S ribosomal protein L3 OS=Pseudomonas entomophila (strain L48) GN=rplC PE=3 SV=1
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