MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 182)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1194 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 163 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1113 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 941 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSESM 569 ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1

-3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 882 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 169 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 206 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
ATPA_PSEPK 882 55489 60 (37) 23 (15) 1.15
ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
1 sameset of ATPA_PSEPK
ATPA_PSEP1 882 55458 60 (37) 23 (15) 1.15
ATP synthase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpA PE=3 SV=1
ATPA_VEREI 206 57757 13 (5) 5 (2) 0.12
ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1
ATPA_RICAH 169 56389 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
7 samesets of ATPA_RICAH
ATPA_RICCN 169 56128 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia conorii GN=atpA PE=3 SV=2
ATPA_RICFE 169 56172 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia felis GN=atpA PE=3 SV=1
ATPA_RICPU 169 56160 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia peacockii (strain Rustic) GN=atpA PE=3 SV=1
ATPA_RICRO 169 56195 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Iowa) GN=atpA PE=3 SV=2
ATPA_RICRS 169 56195 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia rickettsii (strain Sheila Smith) GN=atpA PE=3 SV=1
ATPA_RICM5 169 56127 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia massiliae (strain Mtu5) GN=atpA PE=3 SV=2
ATPA_RICB8 169 56616 14 (9) 5 (4) 0.22
ATP synthase subunit alpha OS=Rickettsia bellii (strain OSU 85-389) GN=atpA PE=3 SV=1

-66 peptide matches (35 non-duplicate, 31 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
322 +1 301.6857 601.3568 601.3548 3.49 0 36 0.29 +1Score > 43 indicates identity
Score > 43 indicates homology		 X K.LSGGIR.T
325 +1 602.3646 601.3573 601.3548 4.28 0 20 0.83 +1Score > 43 indicates identity
Score > 32 indicates homology		 X K.LSGGIR.T
333   606.2912 605.2839 605.2809 4.95 0 13 0.83 +1Score > 41 indicates identity
Score > 25 indicates homology		 U X K.ATQTW.-
395 +1 318.1804 634.3462 634.3438 3.79 0 32 0.042 +1Score > 41 indicates identity
Score > 30 indicates homology		 U X E.LAQFR.E + Deamidated (NQ)
541 +1 354.1965 706.3784 706.3762 3.18 0 35 0.18 +1Score > 41 indicates identity X X K.QAVAYR.Q
542   707.3871 706.3798 706.3762 5.12 0 25 0.6 +1Score > 39 indicates identity
Score > 35 indicates homology		 X X K.QAVAYR.Q
584   365.7346 729.4546 729.4497 6.77 1 16 2.3 +3Score > 36 indicates identity
Score > 32 indicates homology		 X K.KLSGGIR.T
742 +1 408.2360 814.4574 814.4548 3.19 1 32 0.71 +4Score > 43 indicates identity X X X R.ELIIGDR.Q
750   822.4498 821.4425 821.4395 3.65 0 33 0.28 +1Score > 40 indicates identity X R.TALAQYR.E
751 +1 411.7289 821.4432 821.4395 4.53 0 28 0.63 +1Score > 40 indicates identity
Score > 39 indicates homology		 X R.TALAQYR.E
794 +2 421.7801 841.5456 841.5385 8.48 0 42 0.011 +1Score > 35 indicates identity U X X R.QISLLLR.R
796   842.5530 841.5457 841.5385 8.57 0 30 0.16 +1Score > 35 indicates identity U X X R.QISLLLR.R
825 +2 427.7730 853.5314 853.5273 4.86 1 43 0.0052 +1Score > 33 indicates identity U X R.ILEVPVGK.E
841   859.5027 858.4954 858.4923 3.63 0 29 0.75 +1Score > 42 indicates identity
Score > 40 indicates homology		 U X R.STVANIVR.K
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_4_20250714120925.raw

Score > 42 indicates identity
842 +1 430.2553 858.4960 858.4923 4.35 0 35 0.26 -1Score > 42 indicates identity U X R.STVANIVR.K
4.35 0 35 0.26 1 STVANLVR  
4.34 0 28 1.3 3 STVAQVVR  
4.34 0 17 15 4 STAVGVGIR  
4.39 0 17 16 5 LSSALAAAR  
17.5 0 15 24 6 LDLTQLR   + Deamidated (NQ)
12.0 1 14 35 7 MRANLVR  
4.32 0 13 37 8 IGVGTGTVR  
-8.73 1 13 39 9 TSARGVLR  
-4.25 0 13 44 10 IIMDVIR  
911   447.7420 893.4694 893.4607 9.80 0 32 0.046 +1Score > 41 indicates identity
Score > 31 indicates homology		 U X K.FTNGAVTGK.T
912 +1 448.2270 894.4394 894.4447 -5.87 0 44 0.0055 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X K.FTNGAVTGK.T + Deamidated (NQ)
916 +2 449.2714 896.5282 896.5232 5.60 0 45 0.0072 +1Score > 36 indicates identity U X K.VAPGVIWR.K
1391 +3 553.8150 1105.6154 1105.6019 12.2 1 44 0.0076 +1Score > 38 indicates identity
Score > 35 indicates homology		 U X R.GFLIDVEVSK.I
1619 +2 599.3301 1196.6456 1196.6401 4.63 0 67 8.8e-005 +1Score > 39 indicates identity U X X R.VVDALGNPIDGK.G
1665 +1 609.3123 1216.6100 1216.6048 4.34 0 69 9.9e-005 +1Score > 42 indicates identity U X R.IDNLDVSSQAR.N
1678 +1 611.3116 1220.6086 1220.6037 4.03 0 43 0.00088 +1Score > 42 indicates identity
Score > 25 indicates homology		 U X K.SVDQPVQTGYK.S
1832   644.8566 1287.6986 1287.6856 10.1 0 46 0.0086 +1Score > 40 indicates identity
Score > 38 indicates homology		 U X K.TAMAIDAIINQK.D
1841 +2 647.3050 1292.5954 1292.5885 5.41 1 102 2.8e-008 +1Score > 40 indicates identity
Score > 39 indicates homology		 U X K.GDFNDEIDAGLK.A
1926 +1 665.3381 1328.6616 1328.6572 3.34 1 96 2.7e-008 +1Score > 41 indicates identity
Score > 33 indicates homology		 U X K.GPLGNTQTDAVEK.V
2128   715.8735 1429.7324 1429.7273 3.57 1 56 0.00032 +1Score > 41 indicates identity
Score > 34 indicates homology		 U X K.GRIDNLDVSSQAR.N
2129   477.5853 1429.7341 1429.7273 4.71 1 31 0.032 +1Score > 41 indicates identity
Score > 29 indicates homology		 U X K.GRIDNLDVSSQAR.N
2133 +2 716.3803 1430.7460 1430.7365 6.64 1 78 3e-006 +1Score > 41 indicates identity
Score > 36 indicates homology		 U X R.NEGTVVSVSDGIVR.I
2365   777.3760 1552.7374 1552.7310 4.13 1 86 1.9e-006 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2366 +1 518.5885 1552.7437 1552.7310 8.14 1 71 5.3e-005 +1Score > 41 indicates identity U X X X R.EAYPGDVFYLHSR.L
2510 +1 538.2963 1611.8671 1611.8409 16.2 1 88 7.1e-007 +1Score > 39 indicates identity U X K.IGSFEQALIAFFNR.D
2512 +1 806.9415 1611.8684 1611.8409 17.1 1 102 4.1e-009 +1Score > 39 indicates identity
Score > 31 indicates homology		 U X K.IGSFEQALIAFFNR.D
2703 +1 854.9290 1707.8434 1707.8315 6.98 1 82 3.2e-006 +1Score > 41 indicates identity
Score > 40 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)
2705 +1 570.2900 1707.8482 1707.8315 9.75 1 35 0.065 +1Score > 40 indicates identity
Score > 35 indicates homology		 U X R.DRGQDALIVYDDLSK.Q + Deamidated (NQ)

+40 subsets and intersections (858 subset proteins in total)

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 450 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 70 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 115 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 RL1_PSEE4 402 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+6

Accession Score Description
1 RPOC_PSEPK 364 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+7

Accession Score Description
1 DBHB_PSEAE 308 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+8

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 290 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 78 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEP1 287 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
2 RL4_PSE14 192 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 OTCC_PSEPK 280 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
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