MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 182)

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+21

Accession Score Description
1 RPOB_PSEPG 190 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1

+22

Accession Score Description
1 ODO2_PSEAE 190 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+23

Accession Score Description
1 ODB2_PSEPU 180 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 176 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 RL2_PSEP1 172 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

+26

Accession Score Description
1 TIG_PSEPK 156 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2

+27

Accession Score Description
1 HTPG_PSEPK 149 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+28

Accession Score Description
1 ILVC_PSEPG 145 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

-29

Accession Score Description
1 RS2_PSEP1 145 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
29.1 RS2_PSEP1 145 27063 17 (7) 9 (5) 0.51
30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
1 sameset of RS2_PSEP1
RS2_PSEPK 145 27063 17 (7) 9 (5) 0.51
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

-17 peptide matches (11 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
568 +1 363.7015 725.3884 725.3860 3.31 0 10 0.34 +1Score > 37 indicates identity
Score > 18 indicates homology		 K.YIFGAR.N
677 +2 389.2491 776.4836 776.4796 5.17 0 36 0.027 +1Score > 33 indicates identity U K.ILFVGTK.R
833 +1 429.2475 856.4804 856.4766 4.45 1 34 0.22 +1Score > 40 indicates identity U K.IVAEQAAR.C
839   430.2496 858.4846 858.4810 4.19 1 55 0.0032 +1Score > 42 indicates identity U R.IAITEANK.L
1093 +1 322.5319 964.5739 964.5705 3.45 1 17 0.34 +1Score > 34 indicates identity
Score > 25 indicates homology		 K.IHIVNLEK.T
1094   483.2946 964.5746 964.5705 4.26 1 13 0.42 +1Score > 34 indicates identity
Score > 21 indicates homology		 K.IHIVNLEK.T
1401   370.5262 1108.5568 1108.5526 3.72 0 30 0.074 +1Score > 41 indicates identity
Score > 32 indicates homology		 K.AGVHFGHQTR.Y
1583   591.8097 1181.6048 1181.5903 12.3 0 25 0.017 +1Score > 40 indicates identity
Score > 20 indicates homology		 U R.WLGGMLTNYK.T
2575   820.4240 1638.8334 1638.8076 15.8 1 60 1.7e-005 +1Score > 40 indicates identity
Score > 24 indicates homology		 U K.TLPMFNDALSFVER.L
4361   774.9094 3095.6085 3095.5663 13.6 1 15 0.22 +1Score > 35 indicates identity
Score > 21 indicates homology		 U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)
4363 +1 1032.8821 3095.6245 3095.5663 18.8 1 70 1.4e-005 +1Score > 34 indicates identity U K.LGIPVIGVVDTNSSPEGVDYIIPGNDDAIR.A + Deamidated (NQ)

+12 subsets and intersections (189 subset proteins in total)

+30

Accession Score Description
1 ACP_PSEPK 139 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1
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