MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 21–30 (out of 182)


Page: Previous 1 2 3 4 5 6 7 8  19 Next 

+21

Accession Score Description
1 RPOB_PSEPG 190 DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1

+22

Accession Score Description
1 ODO2_PSEAE 190 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1

+23

Accession Score Description
1 ODB2_PSEPU 180 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+24

Accession Score Description
1 RL5_PSEE4 176 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1

+25

Accession Score Description
1 RL2_PSEP1 172 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1

-26

Accession Score Description
1 TIG_PSEPK 156 Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
Score Mass Matches Sequences emPAI
26.1 TIG_PSEPK 156 48487 6 (3) 5 (3) 0.15
Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2
2 samesets of TIG_PSEPK
TIG_PSEP1 156 48429 6 (3) 5 (3) 0.15
Trigger factor OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tig PE=3 SV=1
TIG_PSEPG 156 48501 5 (3) 4 (3) 0.15
Trigger factor OS=Pseudomonas putida (strain GB-1) GN=tig PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1114   486.7993 971.5840 971.5764 7.89 0 36 0.098 +2Score > 39 indicates identity K.GTLLVLGSGR.M
1536   582.3273 1162.6400 1162.6346 4.69 0 42 0.0018 +1Score > 39 indicates identity
Score > 27 indicates homology
U K.LNPAGAPAVEPK.S
1604   397.5410 1189.6012 1189.5979 2.76 1 10 1.5 +5Score > 42 indicates identity
Score > 24 indicates homology
U K.QFELKPDDAK.V
1731 +1 619.9155 1237.8164 1237.8010 12.5 1 46 0.00012 +1Score > 19 indicates identity U R.VVLGLIVAEVVK.Q
2564   817.3984 1632.7822 1632.7744 4.83 0 116 6.2e-011 +1Score > 41 indicates identity
Score > 27 indicates homology
U R.AAQNDDQVNIDFVGK.V

3 subsets and intersections (14 subset proteins in total)

Score Mass Subset of
TIG_PSEE4 65 48359 26.1
Trigger factor OS=Pseudomonas entomophila (strain L48) GN=tig PE=3 SV=1
2 samesets of TIG_PSEE4
TIG_PSEMY 65 48300
Trigger factor OS=Pseudomonas mendocina (strain ymp) GN=tig PE=3 SV=1
TIG_PSEPW 65 48456
Trigger factor OS=Pseudomonas putida (strain W619) GN=tig PE=3 SV=1
TIG_PSEA7 46 48518 26.1
Trigger factor OS=Pseudomonas aeruginosa (strain PA7) GN=tig PE=3 SV=1
7 samesets of TIG_PSEA7
TIG_PSEAB 46 48552
Trigger factor OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=tig PE=3 SV=1
TIG_PSEAE 46 48552
Trigger factor OS=Pseudomonas aeruginosa GN=tig PE=3 SV=1
TIG_PSEA8 46 48552
Trigger factor OS=Pseudomonas aeruginosa (strain LESB58) GN=tig PE=3 SV=1
TIG_PSEU5 46 48281
Trigger factor OS=Pseudomonas stutzeri (strain A1501) GN=tig PE=3 SV=1
TIG_PSEFS 46 48316
Trigger factor OS=Pseudomonas fluorescens (strain SBW25) GN=tig PE=3 SV=1
TIG_PSEPF 46 48455
Trigger factor OS=Pseudomonas fluorescens (strain Pf0-1) GN=tig PE=3 SV=1
TIG_PSEF5 46 48539
Trigger factor OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=tig PE=3 SV=1
TIG_PSE14 42 48654 26.1
Trigger factor OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=tig PE=3 SV=1
2 samesets of TIG_PSE14
TIG_PSESM 42 48616
Trigger factor OS=Pseudomonas syringae pv. tomato GN=tig PE=3 SV=1
TIG_PSEU2 42 48644
Trigger factor OS=Pseudomonas syringae pv. syringae (strain B728a) GN=tig PE=3 SV=1

+27

Accession Score Description
1 HTPG_PSEPK 149 Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1

+28

Accession Score Description
1 ILVC_PSEPG 145 Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1

+29

Accession Score Description
1 RS2_PSEP1 145 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+30

Accession Score Description
1 ACP_PSEPK 139 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1
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