MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 190 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 190 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 180 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 176 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 172 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 25.1 | RL2_PSEP1 |
172 | 29822 | 12 (4) | 8 (3) | 0.25 | |
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 | |||||||
| 4 samesets of RL2_PSEP1 | |||||||
| RL2_PSEPK |
172 | 29822 | 12 (4) | 8 (3) | 0.25 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain KT2440) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPW |
172 | 29822 | 12 (4) | 8 (3) | 0.25 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain W619) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEE4 |
172 | 29808 | 11 (4) | 7 (3) | 0.25 | ||
| 50S ribosomal protein L2 OS=Pseudomonas entomophila (strain L48) GN=rplB PE=3 SV=1 | |||||||
| RL2_PSEPG |
172 | 29794 | 9 (4) | 6 (3) | 0.25 | ||
| 50S ribosomal protein L2 OS=Pseudomonas putida (strain GB-1) GN=rplB PE=3 SV=1 | |||||||
12 peptide matches (9 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 349 |
615.3593 | 614.3520 | 614.3500 | 3.31 | 0 | 23 | 0.37 | 1Score > 40 indicates identity Score > 32 indicates homology |
K.GAQIAR.S | ||
| 416 |
1 |
325.1882 | 648.3618 | 648.3595 | 3.60 | 0 | 31 | 0.045 | 1Score > 40 indicates identity Score > 30 indicates homology |
U | R.LVDFR.R |
| 538 |
352.7217 | 703.4288 | 703.4268 | 2.86 | 0 | 28 | 0.12 | 1Score > 35 indicates identity Score > 31 indicates homology |
R.YIIAPK.G | ||
| 1005 |
1 |
468.7625 | 935.5104 | 935.5076 | 3.01 | 1 | 24 | 0.13 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | R.EGVYVTLR.L |
| 1278 |
522.7980 | 1043.5814 | 1043.5723 | 8.74 | 0 | 61 | 0.00013 | 1Score > 41 indicates identity Score > 35 indicates homology |
U | R.SAGASAQLIAR.E | |
| 1280 |
523.2892 | 1044.5638 | 1044.5563 | 7.19 | 0 | 23 | 1.6 | 2Score > 42 indicates identity Score > 37 indicates homology |
U | R.SAGASAQLIAR.E + Deamidated (NQ) | |
| 1758 |
626.8311 | 1251.6476 | 1251.6401 | 6.06 | 0 | 32 | 0.12 | 1Score > 40 indicates identity Score > 35 indicates homology |
R.HPVSPWGFPTK.G | ||
| 2432 |
1 |
790.9656 | 1579.9166 | 1579.8933 | 14.8 | 0 | 97 | 2.3e-008 | 1Score > 33 indicates identity |
U | K.GVSAGDQLIAGALAPIK.A |
| 2791 |
440.7602 | 1759.0117 | 1758.9992 | 7.10 | 1 | 8 | 0.87 | 1Score > 32 indicates identity Score > 20 indicates homology |
U | R.NIPVGSTIHGIELKPGK.G |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL2_PSE14 |
60 | 29789 | 25.1 | ||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplB PE=3 SV=1 | |||||
| 5 samesets of RL2_PSE14 | |||||
| RL2_PSEFS |
60 | 29803 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain SBW25) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEPF |
60 | 29773 | |||
| 50S ribosomal protein L2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplB PE=3 SV=1 | |||||
| RL2_PSESM |
60 | 29833 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. tomato GN=rplB PE=3 SV=1 | |||||
| RL2_PSEU2 |
60 | 29789 | |||
| 50S ribosomal protein L2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplB PE=3 SV=1 | |||||
| RL2_PSEMY |
60 | 29847 | |||
| 50S ribosomal protein L2 OS=Pseudomonas mendocina (strain ymp) GN=rplB PE=3 SV=1 | |||||
| ACSL1_HUMAN |
31 | 0 | 25.1 | ||
| description | |||||
| 123 samesets of ACSL1_HUMAN |
|||||
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 156 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 149 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 145 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 145 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 139 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
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