MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 190 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 190 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 180 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 176 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 24.1 | RL5_PSEE4 |
176 | 20385 | 13 (10) | 6 (4) | 0.72 | |
| 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 | |||||||
| 4 samesets of RL5_PSEE4 | |||||||
| RL5_PSEP1 |
176 | 20316 | 13 (10) | 6 (4) | 0.73 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPG |
176 | 20316 | 13 (10) | 6 (4) | 0.73 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain GB-1) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPK |
176 | 20316 | 13 (10) | 6 (4) | 0.73 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain KT2440) GN=rplE PE=3 SV=1 | |||||||
| RL5_PSEPW |
176 | 20399 | 13 (10) | 6 (4) | 0.72 | ||
| 50S ribosomal protein L5 OS=Pseudomonas putida (strain W619) GN=rplE PE=3 SV=1 | |||||||
13 peptide matches (7 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 473 |
340.6805 | 679.3464 | 679.3442 | 3.32 | 1 | 9 | 2.4 | 7Score > 40 indicates identity Score > 25 indicates homology |
K.FPFRN.- | ||
| 613 |
375.7303 | 749.4460 | 749.4436 | 3.32 | 1 | 23 | 0.39 | 1Score > 36 indicates identity Score > 32 indicates homology |
R.KSIAGFK.V | ||
| 885 |
2 |
441.7966 | 881.5786 | 881.5698 | 10.0 | 0 | 53 | 0.00018 | 1Score > 28 indicates identity |
U | R.LLAISLPR.V |
| 889 |
1 |
443.2480 | 884.4814 | 884.4756 | 6.61 | 1 | 32 | 0.034 | 1Score > 39 indicates identity Score > 29 indicates homology |
U | R.EGWPIGVK.V |
| 1531 |
2 |
581.3328 | 1160.6510 | 1160.6401 | 9.43 | 0 | 47 | 0.0081 | 1Score > 39 indicates identity |
U | R.GLDITLTTTAR.S |
| 1890 |
1 |
439.5961 | 1315.7665 | 1315.7612 | 3.98 | 0 | 52 | 4.5e-005 | 1Score > 34 indicates identity Score > 21 indicates homology |
U | K.ITGQKPVVTFAR.K |
| 1892 |
658.8913 | 1315.7680 | 1315.7612 | 5.18 | 0 | 39 | 0.0035 | 1Score > 34 indicates identity Score > 27 indicates homology |
U | K.ITGQKPVVTFAR.K |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL5_AZOVD |
56 | 20433 | 24.1 | ||
| 50S ribosomal protein L5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplE PE=3 SV=1 | |||||
| 6 samesets of RL5_AZOVD | |||||
| RL5_PSEU5 |
56 | 20384 | |||
| 50S ribosomal protein L5 OS=Pseudomonas stutzeri (strain A1501) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEA7 |
56 | 20393 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain PA7) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEA8 |
56 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEAB |
56 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEAE |
56 | 20379 | |||
| 50S ribosomal protein L5 OS=Pseudomonas aeruginosa GN=rplE PE=3 SV=1 | |||||
| RL5_PSEMY |
56 | 20437 | |||
| 50S ribosomal protein L5 OS=Pseudomonas mendocina (strain ymp) GN=rplE PE=3 SV=1 | |||||
| RL5_PSE14 |
32 | 20460 | 24.1 | ||
| 50S ribosomal protein L5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplE PE=3 SV=1 | |||||
| 5 samesets of RL5_PSE14 | |||||
| RL5_PSEF5 |
32 | 20474 | |||
| 50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEFS |
32 | 20490 | |||
| 50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain SBW25) GN=rplE PE=3 SV=1 | |||||
| RL5_PSEPF |
32 | 20474 | |||
| 50S ribosomal protein L5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplE PE=3 SV=1 | |||||
| RL5_PSESM |
32 | 20407 | |||
| 50S ribosomal protein L5 OS=Pseudomonas syringae pv. tomato GN=rplE PE=3 SV=1 | |||||
| RL5_PSEU2 |
32 | 20460 | |||
| 50S ribosomal protein L5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplE PE=3 SV=1 | |||||
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 172 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 156 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 149 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 145 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 145 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 139 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
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