MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 190 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 190 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 180 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 23.1 | ODB2_PSEPU |
180 | 45158 | 9 (6) | 7 (4) | 0.28 | |
| Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 | |||||||
9 peptide matches (8 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 599 |
371.2545 | 740.4944 | 740.4908 | 4.87 | 0 | 31 | 0.075 | 1Score > 32 indicates identity |
U | R.ALVVALR.D | |
| 1068 |
479.2748 | 956.5350 | 956.5291 | 6.25 | 1 | 29 | 0.3 | 1Score > 41 indicates identity Score > 36 indicates homology |
U | R.ALDAGIELR.Y | |
| 1172 |
334.1719 | 999.4939 | 999.4886 | 5.24 | 0 | 12 | 0.63 | 2Score > 40 indicates identity Score > 22 indicates homology |
U | R.YVHGSGPAGR.I | |
| 1325 |
1 |
536.3578 | 1070.7010 | 1070.6852 | 14.8 | 0 | 48 | 0.0002 | 1Score > 23 indicates identity |
U | K.LTLLPFLVR.A |
| 1765 |
628.9157 | 1255.8168 | 1255.8016 | 12.1 | 1 | 27 | 0.0072 | 1Score > 18 indicates identity |
U | R.GKLTLLPFLVR.A | |
| 1766 |
419.6129 | 1255.8169 | 1255.8016 | 12.1 | 1 | 40 | 0.00036 | 1Score > 18 indicates identity |
U | R.GKLTLLPFLVR.A | |
| 1935 |
445.9193 | 1334.7361 | 1334.7306 | 4.07 | 0 | 35 | 0.0029 | 1Score > 38 indicates identity Score > 22 indicates homology |
U | R.QPGDKPLASPAVR.K | |
| 3314 |
1055.5149 | 2109.0152 | 2109.0015 | 6.54 | 1 | 99 | 8.7e-009 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | R.DFPQINATYDDEAQIITR.H |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ODB2_PSEAE |
96 | 45746 | 23.1 | ||
| Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas aeruginosa GN=bkdB PE=1 SV=1 | |||||
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 176 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 172 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 156 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 149 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 145 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 145 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 139 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
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