MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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21| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOB_PSEPG | 190 | DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 21.1 | RPOB_PSEPG |
190 | 151555 | 27 (10) | 20 (7) | 0.13 | |
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain GB-1) GN=rpoB PE=3 SV=1 | |||||||
| 4 samesets of RPOB_PSEPG | |||||||
| RPOB_PSEPK |
190 | 151468 | 27 (10) | 20 (7) | 0.13 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain KT2440) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEP1 |
190 | 151468 | 27 (10) | 20 (7) | 0.13 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEPW |
190 | 151379 | 26 (10) | 19 (7) | 0.13 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas putida (strain W619) GN=rpoB PE=3 SV=1 | |||||||
| RPOB_PSEE4 |
190 | 151230 | 25 (10) | 19 (7) | 0.13 | ||
| DNA-directed RNA polymerase subunit beta OS=Pseudomonas entomophila (strain L48) GN=rpoB PE=3 SV=1 | |||||||
27 peptide matches (21 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 255 |
1 |
516.3051 | 515.2978 | 515.2955 | 4.54 | 0 | 13 | 1.5 | 4Score > 47 indicates identity Score > 27 indicates homology |
R.ALAIE.K | |
| 555 |
358.7200 | 715.4254 | 715.4228 | 3.65 | 0 | 34 | 0.18 | 1Score > 43 indicates identity Score > 39 indicates homology |
U | K.TLVDIR.N | |
| 564 |
361.7151 | 721.4156 | 721.4122 | 4.71 | 0 | 22 | 0.26 | 1Score > 36 indicates identity Score > 29 indicates homology |
K.LLYSAR.I | ||
| 594 |
367.7333 | 733.4520 | 733.4486 | 4.65 | 0 | 17 | 0.39 | 1Score > 35 indicates identity Score > 26 indicates homology |
U | K.VYLAIR.R | |
| 683 |
392.7379 | 783.4612 | 783.4603 | 1.23 | 0 | 32 | 0.077 | 1Score > 37 indicates identity Score > 34 indicates homology |
R.QAVPTLR.A | ||
| 884 |
441.2474 | 880.4802 | 880.4766 | 4.10 | 1 | 19 | 0.29 | 1Score > 38 indicates identity Score > 26 indicates homology |
U | R.HINQLEK.A | |
| 1059 |
317.8635 | 950.5687 | 950.5661 | 2.66 | 0 | 23 | 0.19 | 1Score > 34 indicates identity Score > 28 indicates homology |
R.VIVSQLHR.S | ||
| 1060 |
318.5079 | 952.5019 | 952.4978 | 4.29 | 0 | 19 | 0.29 | 1Score > 39 indicates identity Score > 27 indicates homology |
K.LNHLVDDK.M | ||
| 1078 |
1 |
480.2906 | 958.5666 | 958.5600 | 6.92 | 0 | 33 | 0.012 | 1Score > 37 indicates identity Score > 27 indicates homology |
U | R.GVTFAVPLR.V |
| 1135 |
494.2656 | 986.5166 | 986.5185 | -1.90 | 1 | 13 | 1.3 | 2Score > 42 indicates identity Score > 27 indicates homology |
U | R.HLNEFTVK.A | |
| 1145 |
496.7632 | 991.5118 | 991.5087 | 3.19 | 0 | 30 | 0.18 | 1Score > 41 indicates identity Score > 35 indicates homology |
U | K.AQQYIVDR.R | |
| 1234 |
514.3019 | 1026.5892 | 1026.5822 | 6.87 | 0 | 51 | 0.0029 | 1Score > 39 indicates identity Score > 38 indicates homology |
U | R.VSALGPGGLTR.E | |
| 1292 |
1 |
527.8207 | 1053.6268 | 1053.6182 | 8.18 | 1 | 35 | 0.013 | 1Score > 36 indicates identity Score > 29 indicates homology |
U | K.LSLELVPQR.L |
| 1508 |
1 |
578.3472 | 1154.6798 | 1154.6659 | 12.1 | 1 | 51 | 0.0019 | 1Score > 36 indicates identity |
U | K.QLIDELVAVR.H |
| 1514 |
1 |
578.8232 | 1155.6318 | 1155.6499 | -15.6 | 1 | 50 | 0.0015 | 1Score > 40 indicates identity Score > 34 indicates homology |
U | K.QLIDELVAVR.H + Deamidated (NQ) |
| 1570 |
392.8542 | 1175.5408 | 1175.5360 | 4.06 | 0 | 32 | 0.0059 | 1Score > 41 indicates identity Score > 22 indicates homology |
U | R.SPGVFFDHDR.G | |
| 1914 |
441.8967 | 1322.6683 | 1322.6579 | 7.85 | 0 | 40 | 0.004 | 1Score > 41 indicates identity Score > 29 indicates homology |
U | K.GIVDDIDHLGNR.R | |
| 1966 |
677.8536 | 1353.6926 | 1353.7140 | -15.8 | 0 | 22 | 2.1 | 2Score > 40 indicates identity Score > 38 indicates homology |
U | K.LQQGDDLAPGVLK.I + Deamidated (NQ) | |
| 2294 |
1 |
505.2856 | 1512.8350 | 1512.8260 | 5.93 | 1 | 23 | 0.57 | 1Score > 38 indicates identity Score > 33 indicates homology |
U | R.SALNGQVVDGGAGLKK.G |
| 2541 |
811.9269 | 1621.8392 | 1621.8312 | 4.99 | 0 | 90 | 4.6e-008 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | R.STGSYSLVTQQPLGGK.A | |
| 3513 |
736.0532 | 2205.1378 | 2205.1106 | 12.3 | 1 | 13 | 0.13 | 1Score > 38 indicates identity Score > 16 indicates homology |
U | K.SVFPIISYSGNAALEYVGYR.L |
11 subsets and intersections (263 subset proteins in total)22| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO2_PSEAE | 190 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex OS=Pseudomonas aeruginosa GN=sucB PE=3 SV=1 |
23| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 180 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
24| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL5_PSEE4 | 176 | 50S ribosomal protein L5 OS=Pseudomonas entomophila (strain L48) GN=rplE PE=3 SV=1 |
25| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_PSEP1 | 172 | 50S ribosomal protein L2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplB PE=3 SV=1 |
26| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TIG_PSEPK | 156 | Trigger factor OS=Pseudomonas putida (strain KT2440) GN=tig PE=3 SV=2 |
27| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HTPG_PSEPK | 149 | Chaperone protein htpG OS=Pseudomonas putida (strain KT2440) GN=htpG PE=3 SV=1 |
28| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEPG | 145 | Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1 |
29| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 145 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
30| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACP_PSEPK | 139 | Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1 |
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