MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 182)

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+11

Accession Score Description
1 SUCC_PSEPK 276 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+12

Accession Score Description
1 RS5_PSEE4 271 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+13

Accession Score Description
1 RPOA_PSEPK 263 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+14

Accession Score Description
1 ARCA_PSEPK 261 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+15

Accession Score Description
1 DHSA_ECOLI 235 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+16

Accession Score Description
1 ADHP_ECOLI 222 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+17

Accession Score Description
1 RL18_PSEP1 217 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 213 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ACON2_PSEAE 201 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

-20

Accession Score Description
1 RL11_PSEP1 200 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
Score Mass Matches Sequences emPAI
20.1 RL11_PSEP1 200 14914 12 (4) 8 (3) 0.56
50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
3 samesets of RL11_PSEP1
RL11_PSEPG 200 14914 12 (4) 8 (3) 0.56
50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1
RL11_PSEPK 200 14914 12 (4) 8 (3) 0.56
50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1
RL11_PSEPW 200 14914 12 (4) 8 (3) 0.56
50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1

+12 peptide matches (8 non-duplicate, 4 duplicate)

3 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
RL11_PSEE4 132 14940 20.1
50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1
RL11_CHRVO 126 15002 20.1
50S ribosomal protein L11 OS=Chromobacterium violaceum GN=rplK PE=3 SV=1
5 samesets of RL11_CHRVO
RL11_PSEMY 126 14959
50S ribosomal protein L11 OS=Pseudomonas mendocina (strain ymp) GN=rplK PE=3 SV=1
RL11_PSEA7 126 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain PA7) GN=rplK PE=3 SV=1
RL11_PSEA8 126 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplK PE=3 SV=1
RL11_PSEAB 126 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplK PE=3 SV=1
RL11_PSEAE 126 14955
50S ribosomal protein L11 OS=Pseudomonas aeruginosa GN=rplK PE=3 SV=1
RL11_ACIF2 46 15022 20.1
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=rplK PE=3 SV=1
5 samesets of RL11_ACIF2
RL11_PSESM 46 14930
50S ribosomal protein L11 OS=Pseudomonas syringae pv. tomato GN=rplK PE=3 SV=1
RL11_PSEU2 46 14930
50S ribosomal protein L11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplK PE=3 SV=1
RL11_PSE14 46 14911
50S ribosomal protein L11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplK PE=3 SV=1
RL11_PRB01 46 14968
50S ribosomal protein L11 OS=Gamma-proteobacterium EBAC31A08 GN=rplK PE=3 SV=1
RL11_ACIF5 46 15022
50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=rplK PE=3 SV=1
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