MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 276 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 271 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 263 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 261 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 235 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 222 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 217 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 213 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 201 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 200 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 20.1 | RL11_PSEP1 |
200 | 14914 | 12 (4) | 8 (3) | 0.56 | |
| 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 | |||||||
| 3 samesets of RL11_PSEP1 | |||||||
| RL11_PSEPG |
200 | 14914 | 12 (4) | 8 (3) | 0.56 | ||
| 50S ribosomal protein L11 OS=Pseudomonas putida (strain GB-1) GN=rplK PE=3 SV=1 | |||||||
| RL11_PSEPK |
200 | 14914 | 12 (4) | 8 (3) | 0.56 | ||
| 50S ribosomal protein L11 OS=Pseudomonas putida (strain KT2440) GN=rplK PE=3 SV=1 | |||||||
| RL11_PSEPW |
200 | 14914 | 12 (4) | 8 (3) | 0.56 | ||
| 50S ribosomal protein L11 OS=Pseudomonas putida (strain W619) GN=rplK PE=3 SV=1 | |||||||
12 peptide matches (8 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 390 |
316.6922 | 631.3698 | 631.3653 | 7.14 | 0 | 24 | 0.6 | 1Score > 41 indicates identity Score > 34 indicates homology |
K.VGTVTR.A | ||
| 469 |
338.1938 | 674.3730 | 674.3711 | 2.85 | 0 | 14 | 0.97 | 1Score > 42 indicates identity Score > 27 indicates homology |
R.TIAGSAR.S | ||
| 838 |
430.2148 | 858.4150 | 858.4123 | 3.16 | 1 | 20 | 0.11 | 1Score > 40 indicates identity Score > 23 indicates homology |
R.SFTFETK.S | ||
| 893 |
1 |
444.2474 | 886.4802 | 886.4760 | 4.83 | 1 | 46 | 0.023 | 1Score > 42 indicates identity |
U | R.AQLEDIAK.A |
| 951 |
2 |
458.2834 | 914.5522 | 914.5437 | 9.38 | 0 | 27 | 0.37 | 1Score > 38 indicates identity Score > 35 indicates homology |
K.STPASVLLK.K | |
| 2125 |
1 |
715.3716 | 1428.7286 | 1428.7209 | 5.45 | 0 | 85 | 1.8e-006 | 1Score > 40 indicates identity |
U | K.QADLTAADLDAAVR.T |
| 2126 |
477.2643 | 1428.7711 | 1428.7685 | 1.82 | 0 | 16 | 0.21 | 1Score > 40 indicates identity Score > 22 indicates homology |
K.AAGLTSGSARPNTVK.V | ||
| 3389 |
1073.5757 | 2145.1368 | 2145.0954 | 19.3 | 1 | 97 | 3.8e-009 | 1Score > 37 indicates identity Score > 25 indicates homology |
U | R.TQGQEAGLPTPVIITVYSDR.S + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RL11_PSEE4 |
132 | 14940 | 20.1 | ||
| 50S ribosomal protein L11 OS=Pseudomonas entomophila (strain L48) GN=rplK PE=3 SV=1 | |||||
| RL11_CHRVO |
126 | 15002 | 20.1 | ||
| 50S ribosomal protein L11 OS=Chromobacterium violaceum GN=rplK PE=3 SV=1 | |||||
| 5 samesets of RL11_CHRVO | |||||
| RL11_PSEMY |
126 | 14959 | |||
| 50S ribosomal protein L11 OS=Pseudomonas mendocina (strain ymp) GN=rplK PE=3 SV=1 | |||||
| RL11_PSEA7 |
126 | 14955 | |||
| 50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain PA7) GN=rplK PE=3 SV=1 | |||||
| RL11_PSEA8 |
126 | 14955 | |||
| 50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplK PE=3 SV=1 | |||||
| RL11_PSEAB |
126 | 14955 | |||
| 50S ribosomal protein L11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplK PE=3 SV=1 | |||||
| RL11_PSEAE |
126 | 14955 | |||
| 50S ribosomal protein L11 OS=Pseudomonas aeruginosa GN=rplK PE=3 SV=1 | |||||
| RL11_ACIF2 |
46 | 15022 | 20.1 | ||
| 50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=rplK PE=3 SV=1 | |||||
| 5 samesets of RL11_ACIF2 | |||||
| RL11_PSESM |
46 | 14930 | |||
| 50S ribosomal protein L11 OS=Pseudomonas syringae pv. tomato GN=rplK PE=3 SV=1 | |||||
| RL11_PSEU2 |
46 | 14930 | |||
| 50S ribosomal protein L11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplK PE=3 SV=1 | |||||
| RL11_PSE14 |
46 | 14911 | |||
| 50S ribosomal protein L11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplK PE=3 SV=1 | |||||
| RL11_PRB01 |
46 | 14968 | |||
| 50S ribosomal protein L11 OS=Gamma-proteobacterium EBAC31A08 GN=rplK PE=3 SV=1 | |||||
| RL11_ACIF5 |
46 | 15022 | |||
| 50S ribosomal protein L11 OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=rplK PE=3 SV=1 | |||||
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