MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SUCC_PSEPK | 276 | Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 271 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOA_PSEPK | 263 | DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARCA_PSEPK | 261 | Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1 |
15| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DHSA_ECOLI | 235 | Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1 |
16| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ADHP_ECOLI | 222 | Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1 |
17| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 217 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
18| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 213 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
19| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACON2_PSEAE | 201 | Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 19.1 | ACON2_PSEAE |
201 | 94196 | 11 (6) | 7 (5) | 0.13 | |
| Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1 | |||||||
11 peptide matches (7 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 651 |
1 |
384.2629 | 766.5112 | 766.5065 | 6.19 | 0 | 29 | 0.031 | 1Score > 27 indicates identity |
U | R.IPLIVGR.G |
| 977 |
463.2824 | 924.5502 | 924.5433 | 7.54 | 0 | 20 | 0.36 | 2Score > 36 indicates identity Score > 28 indicates homology |
U | R.LWLAPPTK.M | |
| 1745 |
622.8325 | 1243.6504 | 1243.6449 | 4.50 | 1 | 37 | 0.0021 | 1Score > 41 indicates identity Score > 22 indicates homology |
U | R.VPPGVDEAAYVK.A | |
| 1913 |
661.8516 | 1321.6886 | 1321.6838 | 3.67 | 0 | 70 | 8.8e-006 | 1Score > 40 indicates identity Score > 32 indicates homology |
U | R.VQTGSTVVSTSTR.N | |
| 2032 |
1 |
694.8546 | 1387.6946 | 1387.6772 | 12.6 | 1 | 64 | 2.3e-005 | 1Score > 41 indicates identity Score > 30 indicates homology |
U | R.YLSFDQIAEFR.E |
| 2623 |
1 |
834.4329 | 1666.8512 | 1666.8315 | 11.8 | 0 | 72 | 2.7e-006 | 1Score > 40 indicates identity Score > 29 indicates homology |
U | K.GFPVAYVGDVVGTGSSR.K |
| 3070 |
1 |
484.2699 | 1933.0505 | 1933.0282 | 11.5 | 0 | 19 | 0.34 | 1Score > 36 indicates identity Score > 27 indicates homology |
U | R.GGVSLRPGDGIIHSWLNR.M |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ACON2_ECOLI |
37 | 94009 | 19.1 | ||
| Aconitate hydratase 2 OS=Escherichia coli (strain K12) GN=acnB PE=1 SV=3 | |||||
| 1 sameset of ACON2_ECOLI | |||||
| ACON2_SYNY3 |
37 | 94290 | |||
| Aconitate hydratase 2 OS=Synechocystis sp. (strain PCC 6803) GN=acnB PE=3 SV=1 | |||||
| RIBA_BAUCH |
29 | 22461 | 19.1 | ||
| GTP cyclohydrolase-2 OS=Baumannia cicadellinicola subsp. Homalodisca coagulata GN=ribA PE=3 SV=1 | |||||
20| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL11_PSEP1 | 200 | 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1 |
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