MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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171| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SECA2_STAHJ | 22 | Protein translocase subunit secA 2 OS=Staphylococcus haemolyticus (strain JCSC1435) GN=secA2 PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 171.1 | SECA2_STAHJ |
22 | 91518 | 7 (1) | 4 (1) | 0.02 | |
| Protein translocase subunit secA 2 OS=Staphylococcus haemolyticus (strain JCSC1435) GN=secA2 PE=3 SV=1 | |||||||
7 peptide matches (4 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 265 |
531.3174 | 530.3101 | 530.3064 | 7.06 | 0 | 18 | 2.9 | 5Score > 47 indicates identity Score > 35 indicates homology |
E.ALQAK.T + Deamidated (NQ) | ||
| 460 |
2 |
337.2044 | 672.3942 | 672.3919 | 3.55 | 0 | 34 | 0.26 | 4Score > 41 indicates identity |
U | K.SIGIQR.D |
| 723 |
401.2515 | 800.4884 | 800.4868 | 2.04 | 1 | 19 | 3.3 | 5Score > 40 indicates identity Score > 37 indicates homology |
U | E.KSIGIQR.D | |
| 1016 |
1 |
470.7918 | 939.5690 | 939.5753 | -6.66 | 0 | 22 | 0.036 | 1Score > 35 indicates identity Score > 20 indicates homology |
U | K.QPVLLITR.T + Deamidated (NQ) |
172| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MURD_CHLCH | 21 | UDP-N-acetylmuramoylalanine--D-glutamate ligase OS=Chlorobium chlorochromatii (strain CaD3) GN=murD PE=3 SV=1 |
173| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LOX4_ORYSJ | 21 | Probable lipoxygenase 4 OS=Oryza sativa subsp. japonica GN=Os03g0700700 PE=2 SV=1 |
174| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | C97B3_ARATH | 21 | Cytochrome P450 97B3 OS=Arabidopsis thaliana GN=CYP97B3 PE=2 SV=2 |
175| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACKA_BUCBP | 21 | Acetate kinase OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=ackA PE=3 SV=1 |
176| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NFUA_PSYIN | 18 | Fe/S biogenesis protein nfuA OS=Psychromonas ingrahamii (strain 37) GN=nfuA PE=3 SV=1 |
177| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | FLRT3_HUMAN | 18 | Leucine-rich repeat transmembrane protein FLRT3 OS=Homo sapiens GN=FLRT3 PE=1 SV=1 |
178| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DYH1_RAT | 18 | Dynein heavy chain 1, axonemal OS=Rattus norvegicus GN=Dnah1 PE=2 SV=2 |
179| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | T2R38_GORGO | 17 | Taste receptor type 2 member 38 OS=Gorilla gorilla gorilla GN=TAS2R38 PE=3 SV=1 |
180| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | XYLH_HAEIN | 16 | Xylose transport system permease protein xylH OS=Haemophilus influenzae GN=xylH PE=3 SV=1 |
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