MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 182)

Page: Previous 1 2 3 4 5 6 7 … 19 Next 

+11

Accession Score Description
1 SUCC_PSEPK 276 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+12

Accession Score Description
1 RS5_PSEE4 271 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+13

Accession Score Description
1 RPOA_PSEPK 263 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+14

Accession Score Description
1 ARCA_PSEPK 261 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+15

Accession Score Description
1 DHSA_ECOLI 235 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+16

Accession Score Description
1 ADHP_ECOLI 222 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

-17

Accession Score Description
1 RL18_PSEP1 217 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
Score Mass Matches Sequences emPAI
17.1 RL18_PSEP1 217 12677 7 (6) 5 (4) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
3 samesets of RL18_PSEP1
RL18_PSEPG 217 12677 7 (6) 5 (4) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain GB-1) GN=rplR PE=3 SV=1
RL18_PSEPK 217 12677 7 (6) 5 (4) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain KT2440) GN=rplR PE=3 SV=1
RL18_PSEPW 217 12665 7 (6) 5 (4) 1.00
50S ribosomal protein L18 OS=Pseudomonas putida (strain W619) GN=rplR PE=3 SV=1

-7 peptide matches (5 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1182   502.7760 1003.5374 1003.5550 -17.5 0 25 0.092 +1Score > 41 indicates identity
Score > 27 indicates homology		 K.VLASASTLDK.D
1418 +1 560.7925 1119.5704 1119.5673 2.83 0 63 0.00014 +1Score > 42 indicates identity
Score > 37 indicates homology		 U K.AAGVSQVAFDR.S
1458   567.2770 1132.5394 1132.5360 3.02 0 66 0.00018 +1Score > 42 indicates identity
Score > 41 indicates homology		 U R.DGATGNIDAATK.V
2035 +1 463.5984 1387.7734 1387.7671 4.54 1 99 3.2e-008 +1Score > 38 indicates identity
Score > 37 indicates homology		 U K.VLASASTLDKDLR.D
2674   564.2867 1689.8383 1689.8322 3.59 0 27 0.0095 +1Score > 41 indicates identity
Score > 19 indicates homology		 U R.SSQHIYAQVISADGSK.V

4 subsets and intersections (13 subset proteins in total)

Score Mass Subset of
RL18_PSEA7 142 12711 17.1
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain PA7) GN=rplR PE=3 SV=1
4 samesets of RL18_PSEA7
RL18_PSEA8 142 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplR PE=3 SV=1
RL18_PSEAB 142 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplR PE=3 SV=1
RL18_PSEAE 142 12711
50S ribosomal protein L18 OS=Pseudomonas aeruginosa GN=rplR PE=3 SV=1
RL18_TERTT 142 12760
50S ribosomal protein L18 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rplR PE=3 SV=1
RL18_PSE14 109 12653 17.1
50S ribosomal protein L18 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplR PE=3 SV=1
3 samesets of RL18_PSE14
RL18_PSEE4 109 12651
50S ribosomal protein L18 OS=Pseudomonas entomophila (strain L48) GN=rplR PE=3 SV=1
RL18_PSESM 109 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. tomato GN=rplR PE=3 SV=1
RL18_PSEU2 109 12653
50S ribosomal protein L18 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplR PE=3 SV=1
RL18_PSEF5 106 12692 17.1
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplR PE=3 SV=1
2 samesets of RL18_PSEF5
RL18_PSEFS 106 12708
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain SBW25) GN=rplR PE=3 SV=1
RL18_PSEPF 106 12692
50S ribosomal protein L18 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplR PE=3 SV=1
RL18_PSEMY 27 12636 17.1
50S ribosomal protein L18 OS=Pseudomonas mendocina (strain ymp) GN=rplR PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 213 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ACON2_PSEAE 201 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+20

Accession Score Description
1 RL11_PSEP1 200 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
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