MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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151| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | LPXC_AZOVD | 31 | UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1 |
152| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OXAA_RUTMC | 31 | Inner membrane protein oxaA OS=Ruthia magnifica subsp. Calyptogena magnifica GN=oxaA PE=3 SV=1 |
153| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_METFK | 30 | Ketol-acid reductoisomerase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=ilvC PE=3 SV=1 |
154| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | IDH_SYNY3 | 30 | Isocitrate dehydrogenase [NADP] OS=Synechocystis sp. (strain PCC 6803) GN=icd PE=1 SV=2 |
155| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | COAX_ACICJ | 29 | Type III pantothenate kinase OS=Acidiphilium cryptum (strain JF-5) GN=coaX PE=3 SV=1 |
156| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS1_PSEAE | 29 | 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1 |
157| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PPK_RHOPT | 27 | Polyphosphate kinase OS=Rhodopseudomonas palustris (strain TIE-1) GN=ppk PE=3 SV=1 |
158| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | HUTI_KLEP3 | 27 | Imidazolonepropionase OS=Klebsiella pneumoniae (strain 342) GN=hutI PE=3 SV=1 |
159| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ACKA_THEAB | 27 | Acetate kinase OS=Thermosipho africanus (strain TCF52B) GN=ackA PE=3 SV=1 |
160| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RAPA_PSEPG | 27 | RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain GB-1) GN=rapA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 160.1 | RAPA_PSEPG |
27 | 106249 | 8 (1) | 4 (1) | 0.02 | |
| RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain GB-1) GN=rapA PE=3 SV=1 | |||||||
| 4 samesets of RAPA_PSEPG | |||||||
| RAPA_PSEPK |
27 | 106215 | 8 (1) | 4 (1) | 0.02 | ||
| RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain KT2440) GN=rapA PE=3 SV=1 | |||||||
| RAPA_PSEP1 |
27 | 106245 | 8 (1) | 4 (1) | 0.02 | ||
| RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rapA PE=3 SV=1 | |||||||
| RAPA_PSEPW |
27 | 106243 | 7 (1) | 3 (1) | 0.02 | ||
| RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain W619) GN=rapA PE=3 SV=1 | |||||||
| RAPA_PSEE4 |
27 | 105980 | 7 (1) | 3 (1) | 0.02 | ||
| RNA polymerase-associated protein rapA OS=Pseudomonas entomophila (strain L48) GN=rapA PE=3 SV=1 | |||||||
8 peptide matches (5 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 518 |
1 |
702.3799 | 701.3726 | 701.3708 | 2.62 | 1 | 35 | 0.061 | 1Score > 45 indicates identity Score > 35 indicates homology |
E.LLDEGR.L | |
| 519 |
2 |
351.6938 | 701.3730 | 701.3708 | 3.22 | 1 | 28 | 0.31 | 1Score > 45 indicates identity Score > 35 indicates homology |
E.LLDEGR.L | |
| 1782 |
633.8801 | 1265.7456 | 1265.7456 | 0.070 | 0 | 8 | 2.2 | 4Score > 34 indicates identity Score > 24 indicates homology |
R.LVALQAVNPSVR.D | ||
| 2106 |
710.3925 | 1418.7704 | 1418.7769 | -4.57 | 0 | 25 | 0.073 | 1Score > 39 indicates identity Score > 26 indicates homology |
R.LLTVLYPATGDTR.Q | ||
| 2781 |
875.4858 | 1748.9570 | 1748.9250 | 18.3 | 0 | 27 | 0.012 | 1Score > 36 indicates identity Score > 20 indicates homology |
U | R.LFAGQIDPLSWFSLR.Y |
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