MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 182)

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+11

Accession Score Description
1 SUCC_PSEPK 276 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+12

Accession Score Description
1 RS5_PSEE4 271 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+13

Accession Score Description
1 RPOA_PSEPK 263 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

+14

Accession Score Description
1 ARCA_PSEPK 261 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+15

Accession Score Description
1 DHSA_ECOLI 235 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

-16

Accession Score Description
1 ADHP_ECOLI 222 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1
Score Mass Matches Sequences emPAI
16.1 ADHP_ECOLI 222 35870 8 (6) 3 (2) 0.28
Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

-8 peptide matches (5 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
498   346.6911 691.3676 691.3653 3.38 0 15 1.3 +3Score > 42 indicates identity
Score > 28 indicates homology		 K.NVFNAK.V
1759   626.8561 1251.6976 1251.6935 3.28 0 70 2e-005 +1Score > 37 indicates identity
Score > 36 indicates homology		 U K.TGGAHAAVVTAVAK.A
1761 +1 418.2404 1251.6994 1251.6935 4.66 0 31 0.032 +1Score > 37 indicates identity
Score > 29 indicates homology		 U K.TGGAHAAVVTAVAK.A
2546   542.9927 1625.9563 1625.9352 12.9 1 74 4e-006 +1Score > 32 indicates identity U R.LVLDGIEVVGSLVGTR.Q
2547 +2 813.9863 1625.9580 1625.9352 14.0 1 91 5.6e-008 +1Score > 31 indicates identity U R.LVLDGIEVVGSLVGTR.Q

1 subset or intersection (11 subset proteins in total)

Score Mass Subset of
ADH_STAA3 185 36424 16.1
Alcohol dehydrogenase OS=Staphylococcus aureus (strain USA300) GN=adh PE=3 SV=1
10 samesets of ADH_STAA3
ADH_STAAB 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) GN=adh PE=3 SV=1
ADH_STAAC 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain COL) GN=adh PE=3 SV=1
ADH_STAAM 185 36438
Alcohol dehydrogenase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=adh PE=3 SV=1
ADH_STAAN 185 36438
Alcohol dehydrogenase OS=Staphylococcus aureus (strain N315) GN=adh PE=1 SV=1
ADH_STAAR 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain MRSA252) GN=adh PE=3 SV=1
ADH_STAAS 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain MSSA476) GN=adh PE=3 SV=1
ADH_STAAW 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain MW2) GN=adh PE=3 SV=1
ADH_STAEQ 185 36827
Alcohol dehydrogenase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=adh PE=3 SV=1
ADH_STAES 185 36896
Alcohol dehydrogenase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=adh PE=3 SV=1
ADH_STAA8 185 36424
Alcohol dehydrogenase OS=Staphylococcus aureus (strain NCTC 8325) GN=adh PE=3 SV=1

+17

Accession Score Description
1 RL18_PSEP1 217 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 213 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ACON2_PSEAE 201 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+20

Accession Score Description
1 RL11_PSEP1 200 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
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