MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 151–160 (out of 182)

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+151

Accession Score Description
1 LPXC_AZOVD 31 UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=lpxC PE=3 SV=1

+152

Accession Score Description
1 OXAA_RUTMC 31 Inner membrane protein oxaA OS=Ruthia magnifica subsp. Calyptogena magnifica GN=oxaA PE=3 SV=1

+153

Accession Score Description
1 ILVC_METFK 30 Ketol-acid reductoisomerase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) GN=ilvC PE=3 SV=1

+154

Accession Score Description
1 IDH_SYNY3 30 Isocitrate dehydrogenase [NADP] OS=Synechocystis sp. (strain PCC 6803) GN=icd PE=1 SV=2

+155

Accession Score Description
1 COAX_ACICJ 29 Type III pantothenate kinase OS=Acidiphilium cryptum (strain JF-5) GN=coaX PE=3 SV=1

-156

Accession Score Description
1 RS1_PSEAE 29 30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
Score Mass Matches Sequences emPAI
156.1 RS1_PSEAE 29 61946 9 (1) 5 (1) 0.04
30S ribosomal protein S1 OS=Pseudomonas aeruginosa GN=rpsA PE=3 SV=1
7 samesets of RS1_PSEAE
RS1_PROSP 29 41702 8 (1) 4 (1) 0.06
30S ribosomal protein S1 (Fragment) OS=Providencia sp. GN=rpsA PE=3 SV=1
RS1_BUCAI 29 62849 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=rpsA PE=3 SV=1
RS1_BUCAP 29 62605 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Buchnera aphidicola subsp. Schizaphis graminum GN=rpsA PE=3 SV=1
RS1_ECO57 29 61235 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O157:H7 GN=rpsA PE=3 SV=1
RS1_ECOL6 29 61235 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli O6 GN=rpsA PE=3 SV=1
RS1_ECOLI 29 61235 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Escherichia coli (strain K12) GN=rpsA PE=1 SV=1
RS1_SHIFL 29 61235 6 (1) 3 (1) 0.04
30S ribosomal protein S1 OS=Shigella flexneri GN=rpsA PE=3 SV=1

-9 peptide matches (6 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
359 +1 308.7061 615.3976 615.3956 3.40 0 24 0.16 +1Score > 42 indicates identity
Score > 29 indicates homology		 R.VSLGLK.Q
384 +2 315.7140 629.4134 629.4112 3.58 0 31 0.2 +1Score > 38 indicates identity
Score > 37 indicates homology		 R.ISLGIK.Q
922   300.8199 899.4379 899.4349 3.35 1 12 1.7 +4Score > 39 indicates identity
Score > 27 indicates homology		 U R.DTTHLEGK.E
923   450.7269 899.4392 899.4349 4.88 1 9 0.9 +1Score > 39 indicates identity
Score > 21 indicates homology		 U R.DTTHLEGK.E
1463   567.7988 1133.5830 1133.5829 0.089 0 40 0.081 +1Score > 42 indicates identity
Score > 42 indicates homology		 U K.GGFTVDVNGIR.A
2312   509.3057 1524.8953 1524.8777 11.6 0 29 0.012 +1Score > 33 indicates identity
Score > 22 indicates homology		 U R.AFLPGSLVDVRPVR.D

+157

Accession Score Description
1 PPK_RHOPT 27 Polyphosphate kinase OS=Rhodopseudomonas palustris (strain TIE-1) GN=ppk PE=3 SV=1

+158

Accession Score Description
1 HUTI_KLEP3 27 Imidazolonepropionase OS=Klebsiella pneumoniae (strain 342) GN=hutI PE=3 SV=1

+159

Accession Score Description
1 ACKA_THEAB 27 Acetate kinase OS=Thermosipho africanus (strain TCF52B) GN=ackA PE=3 SV=1

+160

Accession Score Description
1 RAPA_PSEPG 27 RNA polymerase-associated protein rapA OS=Pseudomonas putida (strain GB-1) GN=rapA PE=3 SV=1
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