MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 182)


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+141

Accession Score Description
1 MFD_STAEQ 34 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

+142

Accession Score Description
1 RS8_PSEP1 34 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1

+143

Accession Score Description
1 SYR_EUBR3 33 Arginyl-tRNA synthetase OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=argS PE=3 SV=1

+144

Accession Score Description
1 Y3044_PYRAE 33 DNA-binding protein PAE3044 OS=Pyrobaculum aerophilum GN=PAE3044 PE=3 SV=1

+145

Accession Score Description
1 ACCC1_BACSU 33 Biotin carboxylase 1 OS=Bacillus subtilis GN=accC1 PE=3 SV=3

+146

Accession Score Description
1 MREB_BACSU 32 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+147

Accession Score Description
1 GLYA1_PSEF5 32 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

-148

Accession Score Description
1 ODP1_PSEAE 32 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2
Score Mass Matches Sequences emPAI
148.1 ODP1_PSEAE 32 99843 3 (1) 3 (1) 0.02
Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
417   326.1774 650.3402 650.3387 2.31 1 18 0.75 +1Score > 44 indicates identity
Score > 29 indicates homology
K.YLAER.R
1000   466.2642 930.5138 930.5134 0.44 1 23 0.44 +1Score > 42 indicates identity
Score > 32 indicates homology
U K.KVDVDSLR.A
1469   379.8732 1136.5978 1136.5938 3.48 1 32 0.0086 +1Score > 40 indicates identity
Score > 23 indicates homology
U R.NTIPVTHEAR.M

+149

Accession Score Description
1 PROA_PELPD 31 Gamma-glutamyl phosphate reductase OS=Pelobacter propionicus (strain DSM 2379) GN=proA PE=3 SV=1

+150

Accession Score Description
1 F16A2_ACAM1 31 Fructose-1,6-bisphosphatase class 1 2 OS=Acaryochloris marina (strain MBIC 11017) GN=fbp2 PE=3 SV=1
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