MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 182)


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+141

Accession Score Description
1 MFD_STAEQ 34 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

-142

Accession Score Description
1 RS8_PSEP1 34 30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
Score Mass Matches Sequences emPAI
142.1 RS8_PSEP1 34 14023 4 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsH PE=3 SV=1
13 samesets of RS8_PSEP1
RS8_PSEPG 34 14023 4 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain GB-1) GN=rpsH PE=3 SV=1
RS8_PSEPK 34 14023 4 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain KT2440) GN=rpsH PE=3 SV=1
RS8_PSEE4 34 14024 4 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas entomophila (strain L48) GN=rpsH PE=3 SV=1
RS8_PSEFS 34 14140 4 (1) 3 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsH PE=3 SV=1
RS8_PSEF5 34 14178 3 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsH PE=3 SV=1
RS8_PSEMY 34 14106 3 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas mendocina (strain ymp) GN=rpsH PE=3 SV=1
RS8_PSEPF 34 14166 3 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsH PE=3 SV=1
RS8_PSEPW 34 14039 3 (1) 2 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas putida (strain W619) GN=rpsH PE=3 SV=1
RS8_PSEA7 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsH PE=3 SV=2
RS8_PSEA8 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsH PE=3 SV=1
RS8_PSEAB 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsH PE=3 SV=1
RS8_PSEAE 34 14219 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas aeruginosa GN=rpsH PE=3 SV=1
RS8_PSEU5 34 14047 1 (1) 1 (1) 0.17
30S ribosomal protein S8 OS=Pseudomonas stutzeri (strain A1501) GN=rpsH PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
228 +1 487.3262 486.3189 486.3166 4.83 0 24 1.4 +6Score > 46 indicates identity
Score > 38 indicates homology
K.VAVAK.V
601   372.2192 742.4238 742.4225 1.81 0 25 0.32 +1Score > 41 indicates identity
Score > 33 indicates homology
U K.AVTDLPK.V
1457   566.3248 1130.6350 1130.6295 4.87 0 34 0.0077 +1Score > 39 indicates identity
Score > 25 indicates homology
U R.GGLGVSIVSTNK.G

+143

Accession Score Description
1 SYR_EUBR3 33 Arginyl-tRNA synthetase OS=Eubacterium rectale (strain ATCC 33656 / VPI 0990) GN=argS PE=3 SV=1

+144

Accession Score Description
1 Y3044_PYRAE 33 DNA-binding protein PAE3044 OS=Pyrobaculum aerophilum GN=PAE3044 PE=3 SV=1

+145

Accession Score Description
1 ACCC1_BACSU 33 Biotin carboxylase 1 OS=Bacillus subtilis GN=accC1 PE=3 SV=3

+146

Accession Score Description
1 MREB_BACSU 32 Rod shape-determining protein mreB OS=Bacillus subtilis GN=mreB PE=3 SV=3

+147

Accession Score Description
1 GLYA1_PSEF5 32 Serine hydroxymethyltransferase 1 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=glyA1 PE=3 SV=1

+148

Accession Score Description
1 ODP1_PSEAE 32 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+149

Accession Score Description
1 PROA_PELPD 31 Gamma-glutamyl phosphate reductase OS=Pelobacter propionicus (strain DSM 2379) GN=proA PE=3 SV=1

+150

Accession Score Description
1 F16A2_ACAM1 31 Fructose-1,6-bisphosphatase class 1 2 OS=Acaryochloris marina (strain MBIC 11017) GN=fbp2 PE=3 SV=1
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