MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 182)

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+11

Accession Score Description
1 SUCC_PSEPK 276 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+12

Accession Score Description
1 RS5_PSEE4 271 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

+13

Accession Score Description
1 RPOA_PSEPK 263 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

-14

Accession Score Description
1 ARCA_PSEPK 261 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1
Score Mass Matches Sequences emPAI
14.1 ARCA_PSEPK 261 46775 19 (10) 12 (6) 0.33
Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

-19 peptide matches (13 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
523   351.7018 701.3890 701.3860 4.28 0 20 1.3 +2Score > 42 indicates identity
Score > 33 indicates homology		 K.WILDR.K
978 +1 463.2919 924.5692 924.5644 5.24 1 23 0.15 +1Score > 34 indicates identity
Score > 27 indicates homology		 U K.EVIVAGLPK.S
1036   316.4922 946.4548 946.4508 4.16 1 14 2.1 +8Score > 42 indicates identity
Score > 30 indicates homology		 U K.YGVHSEAGK.L
1037   474.2354 946.4562 946.4508 5.72 1 47 0.018 +1Score > 42 indicates identity U K.YGVHSEAGK.L
1187   504.7437 1007.4728 1007.4713 1.57 0 18 1.3 +2Score > 42 indicates identity
Score > 31 indicates homology		 U R.DHFDFVTK.M
1355 +1 543.7891 1085.5636 1085.5505 12.1 1 35 0.065 +1Score > 41 indicates identity
Score > 36 indicates homology		 U R.SWLEGLEPR.H
1372 +1 548.2954 1094.5762 1094.5720 3.88 0 57 0.00096 +1Score > 39 indicates identity U R.NTYTNTLLR.K
1748   623.3665 1244.7184 1244.7017 13.5 1 25 0.031 +1Score > 36 indicates identity
Score > 22 indicates homology		 U R.DLVTVFPEVVK.E
1818   640.8613 1279.7080 1279.7023 4.45 1 22 0.19 +1Score > 37 indicates identity
Score > 28 indicates homology		 U R.QETLLTTAIYK.F
2063 +2 701.4059 1400.7972 1400.7776 14.0 0 73 7.4e-006 +1Score > 37 indicates identity
Score > 34 indicates homology		 U R.QAIGQLAQNLFAK.G
2407 +1 785.9335 1569.8524 1569.8363 10.3 1 61 1.3e-005 +1Score > 39 indicates identity
Score > 25 indicates homology		 U K.ITPDTVGVGLTNEVR.S
3222   680.0344 2037.0814 2037.0565 12.2 1 25 0.92 +1Score > 37 indicates identity U R.GVDVLEMHNLLTDIVQNK.E
3839   816.0689 2445.1849 2445.1448 16.4 1 56 0.0011 +1Score > 39 indicates identity U R.EQWDDGNNVVAIEPGVVIGYDR.N + Deamidated (NQ)

4 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ARCA_PSEPU 90 46933 14.1
Arginine deiminase OS=Pseudomonas putida GN=arcA PE=1 SV=1
ARCA1_RHIME 72 46140 14.1
Arginine deiminase 1 OS=Rhizobium meliloti GN=arcA1 PE=3 SV=1
2 samesets of ARCA1_RHIME
ARCA_RHIET 72 46140
Arginine deiminase OS=Rhizobium etli GN=arcA PE=3 SV=1
ARCA_RHIE6 72 46196
Arginine deiminase OS=Rhizobium etli (strain CIAT 652) GN=arcA PE=3 SV=1
ARCA2_RHIME 69 46880 14.1
Arginine deiminase 2 OS=Rhizobium meliloti GN=arcA2 PE=3 SV=1
6 samesets of ARCA2_RHIME
ARCA_BURMA 69 46422
Arginine deiminase OS=Burkholderia mallei GN=arcA PE=3 SV=1
ARCA_BURPS 69 46422
Arginine deiminase OS=Burkholderia pseudomallei GN=arcA PE=3 SV=1
ARCA_PSEMY 69 46334
Arginine deiminase OS=Pseudomonas mendocina (strain ymp) GN=arcA PE=3 SV=1
ARCA_BRAJA 69 46780
Arginine deiminase OS=Bradyrhizobium japonicum GN=arcA PE=3 SV=1
ARCA_MARMS 69 46613
Arginine deiminase OS=Marinomonas sp. (strain MWYL1) GN=arcA PE=3 SV=1
ARCA_RHILO 69 46031
Arginine deiminase OS=Rhizobium loti GN=arcA PE=3 SV=1
ARCA_PSEAE 66 46806 14.1
Arginine deiminase OS=Pseudomonas aeruginosa GN=arcA PE=1 SV=2

+15

Accession Score Description
1 DHSA_ECOLI 235 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+16

Accession Score Description
1 ADHP_ECOLI 222 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+17

Accession Score Description
1 RL18_PSEP1 217 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 213 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ACON2_PSEAE 201 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+20

Accession Score Description
1 RL11_PSEP1 200 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
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