MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 131–140 (out of 182)

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+131

Accession Score Description
1 RS7_AZOVD 37 30S ribosomal protein S7 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsG PE=3 SV=1

+132

Accession Score Description
1 ODBB_PSEPU 37 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

-133

Accession Score Description
1 PANC_SHESM 37 Pantothenate synthetase OS=Shewanella sp. (strain MR-4) GN=panC PE=3 SV=1
Score Mass Matches Sequences emPAI
133.1 PANC_SHESM 37 30597 4 (1) 3 (1) 0.08
Pantothenate synthetase OS=Shewanella sp. (strain MR-4) GN=panC PE=3 SV=1
9 samesets of PANC_SHESM
PANC_SHESR 37 30625 4 (1) 3 (1) 0.08
Pantothenate synthetase OS=Shewanella sp. (strain MR-7) GN=panC PE=3 SV=1
PANC_SHEON 37 30587 3 (1) 2 (1) 0.08
Pantothenate synthetase OS=Shewanella oneidensis GN=panC PE=3 SV=1
PANC_SHEPC 37 30691 3 (1) 2 (1) 0.08
Pantothenate synthetase OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=panC PE=3 SV=1
PANC_SHESA 37 30460 3 (1) 2 (1) 0.08
Pantothenate synthetase OS=Shewanella sp. (strain ANA-3) GN=panC PE=3 SV=1
PANC_SHESW 37 30691 3 (1) 2 (1) 0.08
Pantothenate synthetase OS=Shewanella sp. (strain W3-18-1) GN=panC PE=3 SV=1
PANC_SHEB2 37 30585 3 (1) 1 (1) 0.08
Pantothenate synthetase OS=Shewanella baltica (strain OS223) GN=panC PE=3 SV=1
PANC_SHEB5 37 30540 3 (1) 1 (1) 0.08
Pantothenate synthetase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=panC PE=3 SV=1
PANC_SHEB8 37 30687 3 (1) 1 (1) 0.08
Pantothenate synthetase OS=Shewanella baltica (strain OS185) GN=panC PE=3 SV=1
PANC_SHEB9 37 30657 3 (1) 1 (1) 0.08
Pantothenate synthetase OS=Shewanella baltica (strain OS195) GN=panC PE=3 SV=1

-4 peptide matches (4 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
654   769.4965 768.4892 768.4857 4.54 1 37 0.03 +1Score > 34 indicates identity U E.KAAAPALK.K
655   385.2519 768.4892 768.4857 4.57 1 4 4.1 +9Score > 34 indicates identity
Score > 22 indicates homology		 U E.KAAAPALK.K
656   385.2545 768.4944 768.4857 11.3 1 9 2.7 +3Score > 31 indicates identity
Score > 26 indicates homology		 U K.AAAPALKK.A
3955   642.0785 2564.2849 2564.3057 -8.12 1 6 0.54 +1Score > 38 indicates identity
Score > 16 indicates homology		 U R.AQGETVAFVPTMGNLHQGHITLVK.E + Deamidated (NQ); Oxidation (M)

+134

Accession Score Description
1 PLSC_NEIGO 36 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Neisseria gonorrhoeae GN=plsC PE=3 SV=1

+135

Accession Score Description
1 ETFD_PSEAE 36 Electron transfer flavoprotein-ubiquinone oxidoreductase OS=Pseudomonas aeruginosa GN=PA2953 PE=1 SV=1

+136

Accession Score Description
1 SODF_PSEAE 35 Superoxide dismutase [Fe] OS=Pseudomonas aeruginosa GN=sodB PE=3 SV=3

+137

Accession Score Description
1 MUC16_HUMAN 35 Mucin-16 OS=Homo sapiens GN=MUC16 PE=1 SV=2

+138

Accession Score Description
1 EFTS_PSEPG 35 Elongation factor Ts OS=Pseudomonas putida (strain GB-1) GN=tsf PE=3 SV=1

+139

Accession Score Description
1 HFQ_PSEPK 35 Protein hfq OS=Pseudomonas putida (strain KT2440) GN=hfq PE=3 SV=1

+140

Accession Score Description
1 RS13_CELJU 35 30S ribosomal protein S13 OS=Cellvibrio japonicus (strain Ueda107) GN=rpsM PE=3 SV=1
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