MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 11–20 (out of 182)


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+11

Accession Score Description
1 SUCC_PSEPK 276 Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

+12

Accession Score Description
1 RS5_PSEE4 271 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

-13

Accession Score Description
1 RPOA_PSEPK 263 DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
Score Mass Matches Sequences emPAI
13.1 RPOA_PSEPK 263 36721 20 (12) 9 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1
2 samesets of RPOA_PSEPK
RPOA_PSEP1 263 36721 20 (12) 9 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpoA PE=3 SV=1
RPOA_PSEE4 263 36721 20 (12) 9 (6) 0.63
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas entomophila (strain L48) GN=rpoA PE=3 SV=1

+20 peptide matches (11 non-duplicate, 9 duplicate)

7 subsets and intersections (186 subset proteins in total)

Score Mass Subset of
RPOA_PSE14 214 36765 13.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpoA PE=3 SV=1
6 samesets of RPOA_PSE14
RPOA_PSEPF 214 36723
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpoA PE=3 SV=1
RPOA_PSEPU 214 36695
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida GN=rpoA PE=3 SV=1
RPOA_PSESM 214 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. tomato GN=rpoA PE=3 SV=1
RPOA_PSEU2 214 36765
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpoA PE=3 SV=1
RPOA_PSEF5 214 36721
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpoA PE=3 SV=1
RPOA_PSEU5 214 36620
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas stutzeri (strain A1501) GN=rpoA PE=3 SV=1
RPOA_PSEA7 210 36741 13.1
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain PA7) GN=rpoA PE=3 SV=1
3 samesets of RPOA_PSEA7
RPOA_PSEAB 210 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpoA PE=3 SV=1
RPOA_PSEAE 210 36741
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas aeruginosa GN=rpoA PE=3 SV=2
RPOA_PSEMY 210 36661
DNA-directed RNA polymerase subunit alpha OS=Pseudomonas mendocina (strain ymp) GN=rpoA PE=3 SV=1
RPOA_SACD2 196 36391 13.1
DNA-directed RNA polymerase subunit alpha OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpoA PE=3 SV=1
RPOA_AZOSB 146 35767 13.1
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain BH72) GN=rpoA PE=3 SV=1
5 samesets of RPOA_AZOSB
RPOA_CELJU 146 36326
DNA-directed RNA polymerase subunit alpha OS=Cellvibrio japonicus (strain Ueda107) GN=rpoA PE=3 SV=1
RPOA_CHRSD 146 37113
DNA-directed RNA polymerase subunit alpha OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpoA PE=3 SV=1
RPOA_THISH 146 36890
DNA-directed RNA polymerase subunit alpha OS=Thioalkalivibrio sp. (strain HL-EbGR7) GN=rpoA PE=3 SV=1
RPOA_AZOSE 146 35666
DNA-directed RNA polymerase subunit alpha OS=Azoarcus sp. (strain EbN1) GN=rpoA PE=3 SV=1
RPOA_LEPCP 146 36343
DNA-directed RNA polymerase subunit alpha OS=Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) GN=rpoA PE=3 SV=1
RPOA_ACIAC 138 36282 13.1
DNA-directed RNA polymerase subunit alpha OS=Acidovorax avenae subsp. citrulli (strain AAC00-1) GN=rpoA PE=3 SV=1
+52 samesets of RPOA_ACIAC
RPOA_BLOFL 45 37420 13.1
DNA-directed RNA polymerase subunit alpha OS=Blochmannia floridanus GN=rpoA PE=3 SV=1
3 samesets of RPOA_BLOFL
RPOA_WIGBR 45 37133
DNA-directed RNA polymerase subunit alpha OS=Wigglesworthia glossinidia brevipalpis GN=rpoA PE=3 SV=1
RPOA_BUCBP 45 37453
DNA-directed RNA polymerase subunit alpha OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=rpoA PE=3 SV=1
RPOA_BUCCC 45 37214
DNA-directed RNA polymerase subunit alpha OS=Buchnera aphidicola subsp. Cinara cedri GN=rpoA PE=3 SV=1
RPOA1_PSYIN 38 36427 13.1
DNA-directed RNA polymerase subunit alpha 1 OS=Psychromonas ingrahamii (strain 37) GN=rpoA1 PE=3 SV=1
+110 samesets of RPOA1_PSYIN

+14

Accession Score Description
1 ARCA_PSEPK 261 Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

+15

Accession Score Description
1 DHSA_ECOLI 235 Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

+16

Accession Score Description
1 ADHP_ECOLI 222 Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

+17

Accession Score Description
1 RL18_PSEP1 217 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

+18

Accession Score Description
1 EFG1_PSEPK 213 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+19

Accession Score Description
1 ACON2_PSEAE 201 Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

+20

Accession Score Description
1 RL11_PSEP1 200 50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1
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