MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 182)


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+121

Accession Score Description
1 TYPH_AZOC5 40 Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1

+122

Accession Score Description
1 PSTB_MYCPN 39 Phosphate import ATP-binding protein pstB OS=Mycoplasma pneumoniae GN=pstB PE=3 SV=1

+123

Accession Score Description
1 RL2_STRM5 39 50S ribosomal protein L2 OS=Stenotrophomonas maltophilia (strain R551-3) GN=rplB PE=3 SV=1

+124

Accession Score Description
1 ARLY_CALS8 39 Argininosuccinate lyase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=argH PE=3 SV=1

+125

Accession Score Description
1 MED23_DANRE 39 Mediator of RNA polymerase II transcription subunit 23 OS=Danio rerio GN=med23 PE=2 SV=2

+126

Accession Score Description
1 PHS_PSEAB 39 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1

+127

Accession Score Description
1 PIFA_ECOLI 38 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

+128

Accession Score Description
1 KV103_HUMAN 38 Ig kappa chain V-I region Bi OS=Homo sapiens PE=1 SV=1

-129

Accession Score Description
1 TLPC_BACSU 38 Methyl-accepting chemotaxis protein tlpC OS=Bacillus subtilis GN=tlpC PE=3 SV=3
Score Mass Matches Sequences emPAI
129.1 TLPC_BACSU 38 61803 3 (1) 2 (1) 0.04
Methyl-accepting chemotaxis protein tlpC OS=Bacillus subtilis GN=tlpC PE=3 SV=3
8 samesets of TLPC_BACSU
Y041_SYNY3 38 108554 3 (1) 2 (1) 0.02
Putative methyl-accepting chemotaxis protein sll0041 OS=Synechocystis sp. (strain PCC 6803) GN=sll0041 PE=3 SV=2
BDLA_PSEAE 38 47066 1 (1) 1 (1) 0.05
Biofilm dispersion protein bdlA OS=Pseudomonas aeruginosa GN=bdlA PE=1 SV=1
DCRA_DESVH 38 73167 1 (1) 1 (1) 0.03
Chemoreceptor protein A OS=Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / NCIMB 8303) GN=dcrA PE=4 SV=2
HEMAT_BACSU 38 48738 1 (1) 1 (1) 0.05
Heme-based aerotactic transducer hemAT OS=Bacillus subtilis GN=hemAT PE=1 SV=1
HLYB_VIBCH 38 60112 1 (1) 1 (1) 0.04
Hemolysin secretion protein OS=Vibrio cholerae GN=hlyB PE=4 SV=2
MCPC_BACSU 38 71986 1 (1) 1 (1) 0.03
Methyl-accepting chemotaxis protein mcpC OS=Bacillus subtilis GN=mcpC PE=1 SV=2
TCPI_VIBCH 38 69156 1 (1) 1 (1) 0.03
Toxin coregulated pilus biosynthesis protein I OS=Vibrio cholerae GN=tcpI PE=3 SV=1
TCPI_VIBC3 38 69158 1 (1) 1 (1) 0.03
Toxin coregulated pilus biosynthesis protein I OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=tcpI PE=4 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1308   531.7846 1061.5546 1061.5506 3.84 1 38 0.007 +1Score > 41 indicates identity
Score > 29 indicates homology
U K.GFAVVADEVR.K
4323   607.5135 3032.5311 3032.4972 11.2 1 3 0.98 +1Score > 36 indicates identity
Score > 16 indicates homology
U R.MKLNDTIQTVQQSALQLASASQQLSAGAE.E + 2 Deamidated (NQ)
4324   607.5138 3032.5326 3032.4972 11.7 1 6 0.75 +1Score > 36 indicates identity
Score > 17 indicates homology
U R.MKLNDTIQTVQQSALQLASASQQLSAGAE.E + 2 Deamidated (NQ)

+130

Accession Score Description
1 RL24_PSEE4 37 50S ribosomal protein L24 OS=Pseudomonas entomophila (strain L48) GN=rplX PE=3 SV=1
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