MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 121–130 (out of 182)


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+121

Accession Score Description
1 TYPH_AZOC5 40 Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1

+122

Accession Score Description
1 PSTB_MYCPN 39 Phosphate import ATP-binding protein pstB OS=Mycoplasma pneumoniae GN=pstB PE=3 SV=1

+123

Accession Score Description
1 RL2_STRM5 39 50S ribosomal protein L2 OS=Stenotrophomonas maltophilia (strain R551-3) GN=rplB PE=3 SV=1

+124

Accession Score Description
1 ARLY_CALS8 39 Argininosuccinate lyase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=argH PE=3 SV=1

+125

Accession Score Description
1 MED23_DANRE 39 Mediator of RNA polymerase II transcription subunit 23 OS=Danio rerio GN=med23 PE=2 SV=2

+126

Accession Score Description
1 PHS_PSEAB 39 Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1

+127

Accession Score Description
1 PIFA_ECOLI 38 Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2

-128

Accession Score Description
1 KV103_HUMAN 38 Ig kappa chain V-I region Bi OS=Homo sapiens PE=1 SV=1
Score Mass Matches Sequences emPAI
128.1 KV103_HUMAN 38 12133 2 (1) 2 (1) 0.20
Ig kappa chain V-I region Bi OS=Homo sapiens PE=1 SV=1
3 samesets of KV103_HUMAN
S6A11_HUMAN 38 72098 2 (1) 2 (1) 0.03
Sodium- and chloride-dependent GABA transporter 3 OS=Homo sapiens GN=SLC6A11 PE=2 SV=1
ARFF_ARATH 38 103706 1 (1) 1 (1) 0.02
Auxin response factor 6 OS=Arabidopsis thaliana GN=ARF6 PE=2 SV=2
MDH_THERP 38 32586 1 (1) 1 (1) 0.07
Malate dehydrogenase OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) GN=mdh PE=3 SV=1

-2 peptide matches (2 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
375   314.6937 627.3728 627.3704 3.89 0 38 0.042 +1Score > 37 indicates identity U E.IGVPSR.F
2789   587.0001 1757.9785 1757.9464 18.2 1 7 5.1 +9Score > 35 indicates identity
Score > 27 indicates homology
U R.NSLIWYQQKPGKAPK.F + Deamidated (NQ)

+129

Accession Score Description
1 TLPC_BACSU 38 Methyl-accepting chemotaxis protein tlpC OS=Bacillus subtilis GN=tlpC PE=3 SV=3

+130

Accession Score Description
1 RL24_PSEE4 37 50S ribosomal protein L24 OS=Pseudomonas entomophila (strain L48) GN=rplX PE=3 SV=1
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