MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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121| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TYPH_AZOC5 | 40 | Putative thymidine phosphorylase OS=Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / ORS 571) GN=AZC_2467 PE=3 SV=1 |
122| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PSTB_MYCPN | 39 | Phosphate import ATP-binding protein pstB OS=Mycoplasma pneumoniae GN=pstB PE=3 SV=1 |
123| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL2_STRM5 | 39 | 50S ribosomal protein L2 OS=Stenotrophomonas maltophilia (strain R551-3) GN=rplB PE=3 SV=1 |
124| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ARLY_CALS8 | 39 | Argininosuccinate lyase OS=Caldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903) GN=argH PE=3 SV=1 |
125| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MED23_DANRE | 39 | Mediator of RNA polymerase II transcription subunit 23 OS=Danio rerio GN=med23 PE=2 SV=2 |
126| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PHS_PSEAB | 39 | Putative pterin-4-alpha-carbinolamine dehydratase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=PA14_53000 PE=3 SV=1 |
127| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PIFA_ECOLI | 38 | Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 127.1 | PIFA_ECOLI |
38 | 85141 | 4 (1) | 3 (1) | 0.03 | |
| Phage T7 exclusion protein OS=Escherichia coli (strain K12) GN=pifA PE=4 SV=2 | |||||||
4 peptide matches (3 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 566 |
1 |
723.3635 | 722.3562 | 722.3520 | 5.80 | 0 | 5 | 1.2 | 9Score > 41 indicates identity Score > 18 indicates homology |
R.TITLME.K + Oxidation (M) | |
| 1273 |
521.8406 | 1041.6666 | 1041.6546 | 11.6 | 0 | 38 | 0.0074 | 1Score > 30 indicates identity |
U | K.TSLLNLILR.N | |
| 2975 |
623.3232 | 1866.9478 | 1866.9224 | 13.6 | 0 | 3 | 3.1 | 3Score > 40 indicates identity Score > 20 indicates homology |
U | E.HGLAQNAVPSPSDALFSR.D + Deamidated (NQ) |
128| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KV103_HUMAN | 38 | Ig kappa chain V-I region Bi OS=Homo sapiens PE=1 SV=1 |
129| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TLPC_BACSU | 38 | Methyl-accepting chemotaxis protein tlpC OS=Bacillus subtilis GN=tlpC PE=3 SV=3 |
130| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL24_PSEE4 | 37 | 50S ribosomal protein L24 OS=Pseudomonas entomophila (strain L48) GN=rplX PE=3 SV=1 |
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