Rita4 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita4 sp
MS data file	:	PRT1270_T-BRSC_4_20250714120925.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:41:34 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita4 sp

MS data file

PRT1270_T-BRSC_4_20250714120925.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:41:34 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,786

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,786

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

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Protein Family Summary

Protein families 11–20 (out of 182)

Previous 1 2 3 4 5 6 7 … 19 Next

11

		Accession	Score	Description
	1	SUCC_PSEPK	276	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

12

		Accession	Score	Description
	1	RS5_PSEE4	271	30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
12.1	RS5_PSEE4	271	17700	13 (8)	5 (3)	0.87
	30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1
	4 samesets of RS5_PSEE4
	RS5_PSEP1	271	17712	13 (8)	5 (3)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1
	RS5_PSEPG	271	17712	13 (8)	5 (3)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1
	RS5_PSEPK	271	17712	13 (8)	5 (3)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1
	RS5_PSEPW	271	17712	13 (8)	5 (3)	0.86
	30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1

13 peptide matches (8 non-duplicate, 5 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
578	1	364.7257	727.4368	727.4341	3.82	0	26	0.11	1Score > 39 indicates identity Score > 29 indicates homology		K.LVQVNR.V
580		365.2130	728.4114	728.4181	-9.10	0	22	0.67	1Score > 42 indicates identity Score > 33 indicates homology		K.LVQVNR.V + Deamidated (NQ)
827		428.2506	854.4866	854.4861	0.59	1	15	0.95	1Score > 37 indicates identity Score > 27 indicates homology		R.EVPAAIQK.A
1128		982.5235	981.5162	981.5131	3.18	0	44	0.0037	1Score > 39 indicates identity Score > 33 indicates homology	U	K.GTTLQYATK.A
1130	1	491.7695	981.5244	981.5131	11.6	0	35	0.015	1Score > 39 indicates identity Score > 29 indicates homology	U	K.GTTLQYATK.A
2083		470.9535	1409.8387	1409.8242	10.3	1	22	0.045	1Score > 33 indicates identity Score > 21 indicates homology	U	R.AVLEVAGVQNVLAK.C
2084	1	705.9293	1409.8440	1409.8242	14.1	1	59	0.0001	1Score > 32 indicates identity	U	R.AVLEVAGVQNVLAK.C
2186	2	734.9162	1467.8178	1467.7974	14.0	0	105	8.1e-009	1Score > 37 indicates identity Score > 36 indicates homology	U	R.IFTFTALTVVGDGK.G

3 subsets and intersections (15 subset proteins in total)

	Score	Mass	Subset of
RS5_AZOVD	253	17763	12.1
30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1
7 samesets of RS5_AZOVD
RS5_PSE14	253	17881
30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1
RS5_PSEF5	253	17699
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1
RS5_PSEFS	253	17732
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1
RS5_PSEMY	253	17708
30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1
RS5_PSEPF	253	17769
30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1
RS5_PSESM	253	17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1
RS5_PSEU2	253	17778
30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1
RS5_PSEU5	214	17649	12.1
30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1
RS5_ALCBS	69	17601	12.1
30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1
5 samesets of RS5_ALCBS
RS5_HAHCH	69	17639
30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1
RS5_PSEA7	69	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1
RS5_PSEA8	69	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1
RS5_PSEAB	69	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1
RS5_PSEAE	69	17671
30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1

13

		Accession	Score	Description
	1	RPOA_PSEPK	263	DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

14

		Accession	Score	Description
	1	ARCA_PSEPK	261	Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

15

		Accession	Score	Description
	1	DHSA_ECOLI	235	Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

16

		Accession	Score	Description
	1	ADHP_ECOLI	222	Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

17

		Accession	Score	Description
	1	RL18_PSEP1	217	50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

18

		Accession	Score	Description
	1	EFG1_PSEPK	213	Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

19

		Accession	Score	Description
	1	ACON2_PSEAE	201	Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

20

		Accession	Score	Description
	1	RL11_PSEP1	200	50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

Previous 1 2 3 4 5 6 7 … 19 Next

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MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 11–20 (out of 182)

11

12

13 peptide matches (8 non-duplicate, 5 duplicate)

3 subsets and intersections (15 subset proteins in total)

13

14

15

16

17

18

19

20