MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 111–120 (out of 182)


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+111

Accession Score Description
1 RAVA_ECOBW 42 ATPase ravA OS=Escherichia coli (strain K12 / BW2952) GN=ravA PE=3 SV=1

+112

Accession Score Description
1 Y475_MYCLB 42 UPF0082 protein MLBr00475 OS=Mycobacterium leprae (strain Br4923) GN=MLBr00475 PE=3 SV=1

+113

Accession Score Description
1 EFTU_ANATD 42 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1

+114

Accession Score Description
1 ASPA_PSEFL 41 Aspartate ammonia-lyase OS=Pseudomonas fluorescens GN=aspA PE=3 SV=1

-115

Accession Score Description
1 AMPA_PSEPG 41 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
Score Mass Matches Sequences emPAI
115.1 AMPA_PSEPG 41 52694 6 (2) 5 (1) 0.04
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain GB-1) GN=pepA PE=3 SV=1
3 samesets of AMPA_PSEPG
AMPA_PSEPK 41 52857 6 (2) 5 (1) 0.04
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1
AMPA_PSEPU 41 52835 6 (2) 5 (1) 0.04
Cytosol aminopeptidase OS=Pseudomonas putida GN=pepA PE=3 SV=1
AMPA_PSEP1 41 52796 5 (2) 4 (1) 0.04
Probable cytosol aminopeptidase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=pepA PE=3 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
626   380.2353 758.4560 758.4538 2.97 0 14 1.2 +5Score > 40 indicates identity
Score > 27 indicates homology
U K.VTLLADK.A
1386   552.8312 1103.6478 1103.6339 12.7 0 12 2 +6Score > 35 indicates identity
Score > 27 indicates homology
R.LIVLNYQGGK.K
1633   401.2504 1200.7294 1200.7230 5.28 1 20 0.76 +2Score > 32 indicates identity
Score > 31 indicates homology
U K.KADKPFVLVGK.G
1787 +1 635.3666 1268.7186 1268.7088 7.73 1 40 0.032 +1Score > 38 indicates identity U K.TATLVIPVGENR.K
2981   624.0290 1869.0652 1869.0472 9.60 0 5 1.7 +2Score > 32 indicates identity
Score > 20 indicates homology
K.GATGRPVPLLTQYLLDR.A

+116

Accession Score Description
1 GLO2_RHOPB 41 Hydroxyacylglutathione hydrolase OS=Rhodopseudomonas palustris (strain BisB18) GN=gloB PE=3 SV=1

+117

Accession Score Description
1 ZIP1_YEAST 41 Synaptonemal complex protein ZIP1 OS=Saccharomyces cerevisiae GN=ZIP1 PE=1 SV=2

+118

Accession Score Description
1 ACSA_ECOL6 41 Acetyl-coenzyme A synthetase OS=Escherichia coli O6 GN=acs PE=3 SV=1

+119

Accession Score Description
1 EFP_PSEPG 41 Elongation factor P OS=Pseudomonas putida (strain GB-1) GN=efp PE=3 SV=1

+120

Accession Score Description
1 CH602_RHOBA 41 60 kDa chaperonin 2 OS=Rhodopirellula baltica GN=groL2 PE=3 SV=1
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