Rita4 sp (Mascot Search Results)

Search metadata
User	:	Jennifer
E-mail	:	[email protected]
Search title	:	Rita4 sp
MS data file	:	PRT1270_T-BRSC_4_20250714120925.mgf
Database	:	SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp	:	12 Aug 2025 at 23:41:34 GMT

Search metadata

User

Jennifer

E-mail

[email protected]

Search title

Rita4 sp

MS data file

PRT1270_T-BRSC_4_20250714120925.mgf

Database

SwissProt 57.15 (515,203 sequences; 181,334,896 residues)

Timestamp

12 Aug 2025 at 23:41:34 GMT

Search parameters
Type of search	:	MS/MS Ion Search
Enzyme	:	GluC_Trypsin
Fixed modifications	:	Carbamidomethyl (C)
Variable modifications	:	Deamidated (NQ), Oxidation (M)
Mass values	:	Monoisotopic
Protein mass	:	Unrestricted
Peptide mass tolerance	:	± 20 ppm
Fragment mass tolerance	:	± 0.1 Da
Max missed cleavages	:	1
Instrument type	:	ESI-FTICR
Number of queries	:	4,786

Search parameters

Type of search

MS/MS Ion Search

Enzyme

GluC_Trypsin

Fixed modifications

Carbamidomethyl (C)

Variable modifications

Deamidated (NQ),

Oxidation (M)

Mass values

Monoisotopic

Protein mass

Unrestricted

Peptide mass tolerance

± 20 ppm

Fragment mass tolerance

± 0.1 Da

Max missed cleavages

Instrument type

ESI-FTICR

Number of queries

4,786

Legend
Dupes	Expect	Rank	U	Peptide
	0.037	2		GAYSLSLR	significant
	9	1		GFFLFVEGGR	top ranking
	6.4e-005	1		GSSIFGLAPGK	significant and top ranking
	1.3e-006	1		SSGTSYPDVLK	peptide is found in all proteins in family member 1
	6.2e-007	1		VCNYVSWIK	peptide is found in some but not all proteins in family member 2
	6.4e-005	1	U	GSSIFGLAPGK	unique
2	5.7e-005	1		LNTLETEEWFFK	peptide has two duplicates
	0.18	1		LNTLETEEWFFK	duplicate peptide

Legend

Dupes

Expect

Rank

Peptide

0.037

GAYSLSLR

significant

GFFLFVEGGR

top ranking

6.4e-005

GSSIFGLAPGK

significant and top ranking

1.3e-006

SSGTSYPDVLK

peptide is found in all proteins in family member 1

6.2e-007

VCNYVSWIK

peptide is found in some but not all proteins in family member 2

6.4e-005

GSSIFGLAPGK

unique

5.7e-005

LNTLETEEWFFK

peptide has two duplicates

0.18

LNTLETEEWFFK

duplicate peptide

Right-facing triangle (

) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (

) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Protein families 11–20 (out of 182)

Previous 1 2 3 4 5 6 7 … 19 Next

11

		Accession	Score	Description
	1	SUCC_PSEPK	276	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1

		Score	Mass	Matches	Sequences	emPAI
11.1	SUCC_PSEPK	276	41499	16 (10)	10 (6)	0.46
	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain KT2440) GN=sucC PE=3 SV=1
	3 samesets of SUCC_PSEPK
	SUCC_PSEP1	276	41499	16 (10)	10 (6)	0.46
	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=sucC PE=3 SV=1
	SUCC_PSEPW	276	41472	15 (10)	9 (6)	0.46
	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain W619) GN=sucC PE=3 SV=1
	SUCC_PSEE4	276	41472	15 (10)	9 (6)	0.46
	Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas entomophila (strain L48) GN=sucC PE=3 SV=1

16 peptide matches (11 non-duplicate, 5 duplicate)

Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
Query	Dupes	Observed	Mr(expt)	Mr(calc)	ppm	M	Score	Expect	Rank	U	Peptide
439	1	334.7274	667.4402	667.4381	3.20	0	26	0.079	1Score > 28 indicates identity Score > 27 indicates homology		K.VPVVVR.L
606	1	374.2434	746.4722	746.4691	4.28	0	33	0.047	1Score > 33 indicates identity	U	K.IFVGLAK.L
809		424.7366	847.4586	847.4552	4.07	0	24	1.4	2Score > 41 indicates identity Score > 38 indicates homology	U	K.QVQQFAK.I
1143	1	496.2744	990.5342	990.5287	5.61	0	40	0.0038	1Score > 41 indicates identity Score > 28 indicates homology	U	K.AFAAQWLGK.N
1175		501.2772	1000.5398	1000.5342	5.66	1	9	0.86	1Score > 42 indicates identity Score > 21 indicates homology	U	K.IGGNEWVVK.A
1467		567.8160	1133.6174	1133.6081	8.28	1	32	0.028	1Score > 40 indicates identity Score > 29 indicates homology	U	K.ELYLGAVVDR.S
1831		430.1914	1287.5524	1287.5480	3.39	0	13	0.52	1Score > 37 indicates identity Score > 22 indicates homology	U	K.TFHDPSQDDAR.E
1963	1	676.3754	1350.7362	1350.7183	13.3	1	75	7.6e-006	1Score > 38 indicates identity Score > 36 indicates homology	U	K.QLFAEYGLPVSK.G
2403	1	785.4324	1568.8502	1568.8311	12.2	0	84	4.3e-007	1Score > 38 indicates identity Score > 33 indicates homology	U	K.ATIDPLVGAQPFQGR.E
2405		523.9577	1568.8513	1568.8311	12.9	0	48	0.00022	1Score > 38 indicates identity Score > 24 indicates homology	U	K.ATIDPLVGAQPFQGR.E
2770		867.9387	1733.8628	1733.8584	2.55	0	81	4.7e-007	1Score > 40 indicates identity Score > 30 indicates homology	U	K.NLVTYQTDANGQPVSK.I

10 subsets and intersections (90 subset proteins in total)

	Score	Mass	Subset of
SUCC_PSEPG	225	41413	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas putida (strain GB-1) GN=sucC PE=3 SV=1
SUCC_PSEF5	205	41599	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=sucC PE=3 SV=1
1 sameset of SUCC_PSEF5
SUCC_PSEFS	205	41547
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain SBW25) GN=sucC PE=3 SV=1
SUCC_PSE14	204	41494	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=sucC PE=3 SV=1
2 samesets of SUCC_PSE14
SUCC_PSESM	204	41472
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. tomato GN=sucC PE=3 SV=1
SUCC_PSEU2	204	41442
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas syringae pv. syringae (strain B728a) GN=sucC PE=3 SV=1
SUCC_PSEA7	153	41772	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain PA7) GN=sucC PE=3 SV=1
3 samesets of SUCC_PSEA7
SUCC_PSEAB	153	41830
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=sucC PE=3 SV=1
SUCC_PSEAE	153	41802
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa GN=sucC PE=3 SV=2
SUCC_PSEA8	153	41802
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas aeruginosa (strain LESB58) GN=sucC PE=3 SV=1
SUCC_PSEPF	135	41527	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas fluorescens (strain Pf0-1) GN=sucC PE=3 SV=1
SUCC_PSEMY	102	41759	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas mendocina (strain ymp) GN=sucC PE=3 SV=1
1 sameset of SUCC_PSEMY
SUCC_PSEU5	102	41761
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Pseudomonas stutzeri (strain A1501) GN=sucC PE=3 SV=1
SUCC_MARAV	100	41495	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=sucC PE=3 SV=1
2 samesets of SUCC_MARAV
SUCC_SACD2	100	41524
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=sucC PE=3 SV=1
SUCC_TERTT	100	41703
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=sucC PE=3 SV=1
SUCC_VIBC3	81	41658	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=sucC PE=3 SV=1
2 samesets of SUCC_VIBC3
SUCC_VIBCH	81	41658
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae GN=sucC PE=3 SV=1
SUCC_VIBCM	81	41658
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Vibrio cholerae serotype O1 (strain M66-2) GN=sucC PE=3 SV=1
SUCC_ALCBS	33	41662	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=sucC PE=3 SV=1
SUCC_ACIAD	32	41521	11.1
Succinyl-CoA ligase [ADP-forming] subunit beta OS=Acinetobacter sp. (strain ADP1) GN=sucC PE=3 SV=1
69 samesets of SUCC_ACIAD

12

		Accession	Score	Description
	1	RS5_PSEE4	271	30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

13

		Accession	Score	Description
	1	RPOA_PSEPK	263	DNA-directed RNA polymerase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=rpoA PE=3 SV=1

14

		Accession	Score	Description
	1	ARCA_PSEPK	261	Arginine deiminase OS=Pseudomonas putida (strain KT2440) GN=arcA PE=3 SV=1

15

		Accession	Score	Description
	1	DHSA_ECOLI	235	Succinate dehydrogenase flavoprotein subunit OS=Escherichia coli (strain K12) GN=sdhA PE=1 SV=1

16

		Accession	Score	Description
	1	ADHP_ECOLI	222	Alcohol dehydrogenase, propanol-preferring OS=Escherichia coli (strain K12) GN=adhP PE=1 SV=1

17

		Accession	Score	Description
	1	RL18_PSEP1	217	50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1

18

		Accession	Score	Description
	1	EFG1_PSEPK	213	Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

19

		Accession	Score	Description
	1	ACON2_PSEAE	201	Aconitate hydratase 2 OS=Pseudomonas aeruginosa GN=acnB PE=3 SV=1

20

		Accession	Score	Description
	1	RL11_PSEP1	200	50S ribosomal protein L11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplK PE=3 SV=1

Previous 1 2 3 4 5 6 7 … 19 Next

Not what you expected? Try the select summary.

MASCOT Search Results

Score distribution

Protein Family Summary

Protein families 11–20 (out of 182)

11

16 peptide matches (11 non-duplicate, 5 duplicate)

10 subsets and intersections (90 subset proteins in total)

12

13

14

15

16

17

18

19

20