MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 101–110 (out of 182)


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-101

Accession Score Description
1 RS4_PSEPW 44 30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1
Score Mass Matches Sequences emPAI
101.1 RS4_PSEPW 44 23287 6 (1) 6 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1
11 samesets of RS4_PSEPW
RS4_PSEP1 44 23270 6 (1) 6 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1
RS4_PSEPG 44 23284 6 (1) 6 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas putida (strain GB-1) GN=rpsD PE=3 SV=1
RS4_PSEPK 44 23270 6 (1) 6 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1
RS4_PSEE4 44 23361 5 (1) 5 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas entomophila (strain L48) GN=rpsD PE=3 SV=1
RS4_PSEPF 44 23246 4 (1) 4 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsD PE=3 SV=1
RS4_PSEF5 44 23289 5 (1) 4 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsD PE=3 SV=1
RS4_PSE14 44 23290 3 (1) 3 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsD PE=3 SV=1
RS4_PSEFS 44 23290 3 (1) 3 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsD PE=3 SV=1
RS4_PSEMY 44 23217 3 (1) 3 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas mendocina (strain ymp) GN=rpsD PE=3 SV=1
RS4_PSESM 44 23290 3 (1) 3 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas syringae pv. tomato GN=rpsD PE=3 SV=1
RS4_PSEU2 44 23290 3 (1) 3 (1) 0.10
30S ribosomal protein S4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsD PE=3 SV=1

-6 peptide matches (6 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
773   417.2207 832.4268 832.4402 -16.1 1 12 1.1 +2Score > 43 indicates identity
Score > 25 indicates homology
U E.KSSNQLR.I + Deamidated (NQ)
811   425.2471 848.4796 848.4756 4.79 1 35 0.095 +1Score > 40 indicates identity
Score > 37 indicates homology
R.IYGVLER.Q
972   461.7491 921.4836 921.4807 3.15 1 31 0.11 +1Score > 41 indicates identity
Score > 34 indicates homology
R.EGTDLFLK.S
1312   534.2610 1066.5074 1066.5043 2.92 0 44 0.0019 +1Score > 41 indicates identity
Score > 29 indicates homology
U R.QSDYGTQLR.E
1856   650.8602 1299.7058 1299.6969 6.90 1 20 0.76 +1Score > 40 indicates identity
Score > 31 indicates homology
U R.IVQALELCAQR.G
3111   657.3724 1969.0954 1969.0745 10.6 0 15 0.12 +1Score > 35 indicates identity
Score > 18 indicates homology
U K.TVNIPSYQVRPGDVVAVR.E

+102

Accession Score Description
1 ODO2_PSEPU 44 Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (Fragment) OS=Pseudomonas putida GN=sucB PE=3 SV=2

+103

Accession Score Description
1 TRYP_PIG 44 Trypsin OS=Sus scrofa PE=1 SV=1

+104

Accession Score Description
1 ATMB_SALTY 43 Magnesium-transporting ATPase, P-type 1 OS=Salmonella typhimurium GN=mgtB PE=2 SV=3

+105

Accession Score Description
1 CTCFL_HUMAN 43 Transcriptional repressor CTCFL OS=Homo sapiens GN=CTCFL PE=1 SV=1

+106

Accession Score Description
1 RL15_STRP2 43 description

+107

Accession Score Description
1 FKBP_DEBHA 43 FK506-binding protein 1 OS=Debaryomyces hansenii GN=FPR1 PE=3 SV=1

+108

Accession Score Description
1 RS18_PSEE4 42 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+109

Accession Score Description
1 RL19_PSEE4 42 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

+110

Accession Score Description
1 BIOD_XYLFA 42 Dethiobiotin synthetase OS=Xylella fastidiosa GN=bioD PE=3 SV=1
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