MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita4 sp |
| MS data file | : | PRT1270_T-BRSC_4_20250714120925.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:41:34 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,786 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1194 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 3 | EFTU_CARRP | 163 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1113 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 941 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 | |
| 2 | ATPB_PSESM | 569 | ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 882 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_RICAH | 169 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_VEREI | 206 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 450 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 70 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 115 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 402 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RPOC_PSEPK | 364 | DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DBHB_PSEAE | 308 | DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | PORF_PSESY | 290 | Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1 | |
| 2 | PORF_PSEAE | 78 | Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1 | |
22 peptide matches (11 non-duplicate, 11 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | 1 | 2 | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | 1 | 2 | Peptide |
| 441 |
2 |
336.1563 | 670.2980 | 670.2962 | 2.68 | 0 | 22 | 0.22 | 1Score > 37 indicates identity Score > 28 indicates homology |
|
|
K.FDFDK.S | |
| 442 |
1 |
671.3057 | 670.2984 | 670.2962 | 3.24 | 0 | 27 | 0.32 | 1Score > 37 indicates identity Score > 35 indicates homology |
|
|
K.FDFDK.S | |
| 443 |
671.3060 | 670.2987 | 670.2962 | 3.69 | 0 | (26) | 0.092 | 1Score > 37 indicates identity Score > 28 indicates homology |
|
|
K.FDFDK.S | ||
| 526 |
351.7062 | 701.3978 | 701.3959 | 2.71 | 0 | 30 | 0.68 | 1Score > 43 indicates identity Score > 41 indicates homology |
U | |
|
R.VeLDVK.F + Deamidated (NQ) | |
| 527 |
1 |
702.4053 | 701.3980 | 701.3959 | 2.96 | 1 | 45 | 0.038 | 1Score > 43 indicates identity |
U | |
|
R.VeLDVK.F |
| 528 |
351.7065 | 701.3984 | 701.3959 | 3.56 | 1 | 31 | 1 | 5Score > 43 indicates identity |
U | |
R.VELDVK.F | ||
| 784 |
1 |
419.7122 | 837.4098 | 837.4055 | 5.24 | 0 | 29 | 0.36 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | |
K.NLADFMK.Q | |
| 1869 |
2 |
436.2396 | 1305.6970 | 1305.6929 | 3.15 | 0 | 46 | 0.00075 | 1Score > 40 indicates identity Score > 28 indicates homology |
U | |
K.SVVKPNSYGDIK.N | |
| 1870 |
1 |
653.8560 | 1305.6974 | 1305.6929 | 3.52 | 0 | 62 | 0.00019 | 1Score > 40 indicates identity Score > 37 indicates homology |
U | |
K.SVVKPNSYGDIK.N | |
| 1967 |
2 |
452.2393 | 1353.6961 | 1353.6816 | 10.7 | 1 | 58 | 3.7e-005 | 1Score > 40 indicates identity Score > 26 indicates homology |
U | |
R.VQLDVKFDFDK.S + Deamidated (NQ) | |
| 3656 |
1151.5668 | 2301.1190 | 2301.0913 | 12.0 | 1 | 127 | 9.7e-011 | 1Score > 39 indicates identity |
U | |
K.NNGNLFGGSIGYFLTDDVELR.L + Deamidated (NQ) | ||
| 3659 |
1152.0645 | 2302.1144 | 2302.0753 | 17.0 | 1 | 94 | 2.8e-008 | 1Score > 39 indicates identity Score > 31 indicates homology |
U | |
K.NNGNLFGGSIGYFLTDDVELR.L + 2 Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| PORF_PSEFL |
45 | 34461 | 8.1 | ||
| Outer membrane porin F OS=Pseudomonas fluorescens GN=oprF PE=1 SV=1 | |||||
| 1 sameset of PORF_PSEFL | |||||
| THIC_THELT |
45 | 47776 | |||
| Phosphomethylpyrimidine synthase OS=Thermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / TMO) GN=thiC PE=3 SV=1 | |||||
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSEP1 | 287 | 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSE14 | 192 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OTCC_PSEPK | 280 | Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3 |
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