MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 182)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1194 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 163 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1113 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 941 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSESM 569 ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 882 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 169 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 206 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 450 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 70 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 115 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 RL1_PSEE4 402 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+6

Accession Score Description
1 RPOC_PSEPK 364 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

-7

Accession Score Description
1 DBHB_PSEAE 308 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
Score Mass Matches Sequences emPAI
7.1 DBHB_PSEAE 308 9081 11 (10) 3 (2) 1.59
DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3
1 sameset of DBHB_PSEAE
DBHB_PSEF5 308 9100 11 (10) 3 (2) 1.59
DNA-binding protein HU-beta OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=hupB PE=3 SV=1

-11 peptide matches (5 non-duplicate, 6 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
215   473.3095 472.3022 472.3009 2.80 0 14 1.3 +3Score > 40 indicates identity
Score > 27 indicates homology		 K.IAAAK.I
2027 +1 462.9382 1385.7928 1385.7766 11.7 1 57 0.00018 +1Score > 36 indicates identity
Score > 32 indicates homology		 U R.ALDAVIESVTGALK.A
2030 +3 693.9054 1385.7962 1385.7766 14.2 1 78 3.2e-006 +1Score > 36 indicates identity
Score > 35 indicates homology		 U R.ALDAVIESVTGALK.A
2286   505.2809 1512.8209 1512.8035 11.5 1 53 0.00076 +1Score > 39 indicates identity
Score > 35 indicates homology		 U K.SELIDAIAASADIPK.A
2290 -2 757.4194 1512.8242 1512.8035 13.7 1 70 5.5e-006 +1Score > 39 indicates identity
Score > 30 indicates homology		 U K.SELIDAIAASADIPK.A
2287   757.4180 1512.8214 1512.8035 11.9 1 (69) 7.1e-006 +1Score > 39 indicates identity
Score > 30 indicates homology		 U K.SELIDAIAASADIPK.A
2289   757.4186 1512.8226 1512.8035 12.7 1 (70) 5.1e-006 +1Score > 39 indicates identity
Score > 30 indicates homology		 U K.SELIDAIAASADIPK.A

+8

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 290 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 78 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEP1 287 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
2 RL4_PSE14 192 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 OTCC_PSEPK 280 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
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