MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 182)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1194 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 163 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1113 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 941 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSESM 569 ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 882 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 169 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 206 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

-4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 450 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 70 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 115 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
CH60_PSEPK 450 56765 49 (21) 19 (10) 0.74
60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
CH601_ECOK1 115 57464 23 (7) 10 (4) 0.26
60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1
+27 samesets of CH601_ECOK1
CH602_SORC5 70 58067 6 (4) 3 (2) 0.12
60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
1 sameset of CH602_SORC5
CH60_GEOLS 70 58655 8 (4) 4 (2) 0.12
60 kDa chaperonin OS=Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) GN=groL PE=3 SV=1

-55 peptide matches (32 non-duplicate, 23 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
229 +1 487.3270 486.3197 486.3166 6.48 0 35 0.54 +1Score > 46 indicates identity
Score > 44 indicates homology		 X X K.VAAVK.A
285   559.3832 558.3759 558.3741 3.30 0 17 0.75 +1Score > 34 indicates identity
Score > 28 indicates homology		 X R.VILSK.E
300 +1 586.3571 585.3498 585.3486 2.09 0 41 0.04 +1Score > 39 indicates identity U X K.ATLGPK.G
322 +1 301.6857 601.3568 601.3547 3.51 0 36 0.29 +1Score > 43 indicates identity
Score > 43 indicates homology		 X X K.ISNIR.E
324   602.3645 601.3572 601.3547 4.14 0 14 0.77 +1Score > 43 indicates identity
Score > 26 indicates homology		 X X K.ISNIR.E
402   322.2112 642.4078 642.4064 2.18 0 24 0.16 +1Score > 42 indicates identity
Score > 28 indicates homology		 U X R.NVVLAK.S
405 +1 643.4160 642.4087 642.4064 3.54 0 34 0.31 +2Score > 41 indicates identity U X R.NVVLAK.S
418 +1 326.6574 651.3002 651.2976 4.01 0 33 0.091 +1Score > 40 indicates identity
Score > 35 indicates homology		 X K.FGDSAR.K
465 +1 338.1837 674.3528 674.3599 -10.5 0 23 1.3 +3Score > 43 indicates identity
Score > 36 indicates homology		 X K.DGVSVAK.E
558 +1 360.1785 718.3424 718.3398 3.63 0 25 0.38 +1Score > 43 indicates identity
Score > 33 indicates homology		 X X X K.APGFGDR.R
584   365.7346 729.4546 729.4497 6.79 1 16 2.7 +4Score > 36 indicates identity
Score > 32 indicates homology		 X X K.KISNIR.E
658   771.4380 770.4307 770.4286 2.71 1 19 0.96 +1Score > 39 indicates identity
Score > 31 indicates homology		 U X R.AVESPLR.Q
659 +1 386.2227 770.4308 770.4286 2.87 1 44 0.02 +1Score > 39 indicates identity U X R.AVESPLR.Q
757 +2 414.2726 826.5306 826.5276 3.65 0 38 0.037 +2Score > 36 indicates identity U X X K.LAGGVAVIK.V
758 +1 827.5380 826.5307 826.5276 3.74 0 40 0.02 +2Score > 36 indicates identity U X X K.LAGGVAVIK.V
965   921.5078 920.5005 920.4967 4.12 0 55 0.0021 +1Score > 40 indicates identity U X X K.SFGAPTITK.D
966 -2 461.2576 920.5006 920.4967 4.25 0 28 0.19 +1Score > 40 indicates identity
Score > 34 indicates homology		 U X X K.SFGAPTITK.D
964   461.2571 920.4996 920.4967 3.16 0 (16) 0.96 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X X K.SFGAPTITK.D
967   461.2578 920.5010 920.4967 4.69 0 (21) 0.24 +1Score > 41 indicates identity
Score > 27 indicates homology		 U X X K.SFGAPTITK.D
982   927.5952 926.5879 926.5800 8.52 0 62 8.8e-005 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
984 +2 464.3028 926.5910 926.5800 11.9 0 55 0.00049 +1Score > 34 indicates identity U X R.ALAAIIDLK.G
1110   972.5765 971.5692 971.5651 4.22 1 64 0.00023 +1Score > 40 indicates identity U X K.ATAAVVAELK.N
1111 +3 486.7921 971.5696 971.5651 4.66 1 56 0.00048 +1Score > 40 indicates identity
Score > 36 indicates homology		 U X K.ATAAVVAELK.N
1196   337.8465 1010.5177 1010.5145 3.14 1 34 0.021 +1Score > 40 indicates identity
Score > 30 indicates homology		 U X X X R.VEDALHATR.A
1197   506.2666 1010.5186 1010.5145 4.11 1 64 0.00022 +1Score > 40 indicates identity U X X X R.VEDALHATR.A
1912   661.8297 1321.6448 1321.6336 8.49 1 15 0.22 +1Score > 41 indicates identity
Score > 21 indicates homology		 X K.DAFENMGAQLVK.E
1923 +1 664.8914 1327.7682 1327.7534 11.2 0 42 0.0029 +1Score > 36 indicates identity
Score > 29 indicates homology		 U X K.MLVGVNVLADAVK.A
2021   693.4014 1384.7882 1384.7860 1.59 1 6 0.48 +1Score > 37 indicates identity
Score > 15 indicates homology		 U X K.MLRGVNVLADAVK.V
2094 +1 707.3124 1412.6102 1412.6055 3.33 1 43 0.018 +1Score > 38 indicates identity U X R.AQIENTTSDYDR.E + Deamidated (NQ)
2282   505.2621 1512.7645 1512.7532 7.44 1 25 0.62 +1Score > 40 indicates identity
Score > 36 indicates homology		 U X K.GDNEDQNVGIALLR.R
2283 +1 757.3901 1512.7656 1512.7532 8.22 1 95 1.9e-007 +1Score > 40 indicates identity U X K.GDNEDQNVGIALLR.R
2432   790.9656 1579.9166 1579.9297 -8.29 1 13 5.7 +4Score > 33 indicates identity X R.GVNVLADAVKVTLGPK.G
2520 +1 807.9026 1613.7906 1613.7897 0.60 0 72 1e-006 +1Score > 40 indicates identity
Score > 25 indicates homology		 U X R.QITANAGDEPSVVADK.V
3787 +1 801.4224 2401.2454 2401.2336 4.88 1 56 0.00066 +1Score > 37 indicates identity U X K.ANDAAGDGTTTATVLAQAIITEGLK.A

+31 subsets and intersections (566 subset proteins in total)

+5

Accession Score Description
1 RL1_PSEE4 402 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+6

Accession Score Description
1 RPOC_PSEPK 364 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+7

Accession Score Description
1 DBHB_PSEAE 308 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+8

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 290 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 78 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEP1 287 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
2 RL4_PSE14 192 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 OTCC_PSEPK 280 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
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