MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 182)

Page: 1 2 3 4 5 6 … 19 Next 

-1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1194 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 163 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1113 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
EFTU2_PSEPK 1194 43793 89 (52) 19 (15) 3.17
Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
1 sameset of EFTU2_PSEPK
EFTU_PSEE4 1194 43793 89 (52) 19 (15) 3.17
Elongation factor Tu OS=Pseudomonas entomophila (strain L48) GN=tuf1 PE=3 SV=1
EFTU1_PSEPK 1113 43810 86 (51) 19 (15) 3.39
Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
3 samesets of EFTU1_PSEPK
EFTU_PSEPG 1113 43810 86 (51) 19 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain GB-1) GN=tuf1 PE=3 SV=1
EFTU_PSEPW 1113 43810 86 (51) 19 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain W619) GN=tuf1 PE=3 SV=1
EFTU_PSEP1 1113 43810 86 (51) 19 (15) 3.39
Elongation factor Tu OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=tuf PE=3 SV=1
EFTU_CARRP 163 44439 11 (8) 2 (2) 0.22
Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

-103 peptide matches (50 non-duplicate, 53 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 3 Peptide
494   689.3967 688.3894 688.3868 3.80 0 30 0.35 +1Score > 41 indicates identity
Score > 38 indicates homology		 X X R.GTVVTGR.I
496 +1 345.2031 688.3916 688.3868 7.03 0 31 0.12 +1Score > 41 indicates identity
Score > 35 indicates homology		 X X R.GTVVTGR.I
518 +1 702.3799 701.3726 701.3708 2.62 1 35 0.061 +1Score > 45 indicates identity
Score > 35 indicates homology		 X X K.LLDEGR.A
519 +2 351.6938 701.3730 701.3708 3.22 1 28 0.31 +1Score > 45 indicates identity
Score > 35 indicates homology		 X X K.LLDEGR.A
671 +3 388.7094 775.4042 775.4017 3.26 0 23 0.037 +1Score > 42 indicates identity
Score > 21 indicates homology		 U X X X R.HTPFFK.G
675   776.4130 775.4057 775.4017 5.17 0 26 0.029 +1Score > 41 indicates identity
Score > 23 indicates homology		 U X X X R.HTPFFK.G
719 +2 401.2465 800.4784 800.4756 3.54 0 42 0.0021 +1Score > 42 indicates identity
Score > 27 indicates homology		 U X X R.TVGAGVVAK.I
721 +2 801.4872 800.4799 800.4756 5.38 0 61 0.00075 +1Score > 42 indicates identity U X X R.TVGAGVVAK.I
856   867.5083 866.5010 866.4974 4.21 1 54 0.0009 +1Score > 36 indicates identity U X X R.EHILLSR.Q
858 +2 434.2580 866.5014 866.4974 4.70 1 39 0.029 +1Score > 36 indicates identity U X X R.EHILLSR.Q
1041 +2 947.5554 946.5481 946.5447 3.58 0 63 4e-005 +1Score > 38 indicates identity
Score > 32 indicates homology		 U X X K.TTLTAALTR.V
1044 +3 474.2819 946.5492 946.5447 4.76 0 81 9e-007 +1Score > 38 indicates identity
Score > 33 indicates homology		 U X X K.TTLTAALTR.V
1184 +1 503.2572 1004.4998 1004.4961 3.77 1 34 0.12 +1Score > 42 indicates identity
Score > 37 indicates homology		 U X X K.TIAMEDGLR.F
1363   544.7822 1087.5498 1087.5444 4.99 1 79 1.3e-005 +1Score > 42 indicates identity U X X R.AGENCGVLLR.G
1364   545.2819 1088.5492 1088.5284 19.1 1 17 1.3 +2Score > 42 indicates identity
Score > 31 indicates homology		 U X X R.AGENCGVLLR.G + Deamidated (NQ)
1510   1156.6323 1155.6250 1155.6176 6.45 1 44 0.01 +1Score > 40 indicates identity
Score > 37 indicates homology		 U X X K.FTAEVYVLSK.E
1512 +6 578.8216 1155.6286 1155.6176 9.59 1 45 0.0016 +1Score > 40 indicates identity
Score > 29 indicates homology		 U X X K.FTAEVYVLSK.E
1716 +2 617.3137 1232.6128 1232.6091 3.07 0 16 0.31 +1Score > 42 indicates identity
Score > 23 indicates homology		 U X X K.GYRPQFYFR.T
1717 +2 411.8791 1232.6155 1232.6091 5.19 0 30 0.015 +1Score > 42 indicates identity
Score > 24 indicates homology		 U X X K.GYRPQFYFR.T
1725   413.5791 1237.7155 1237.7030 10.1 1 36 0.056 +2Score > 36 indicates identity U X X R.VQDPLEIVGLR.D
1726 +2 619.8663 1237.7180 1237.7030 12.1 1 69 2.3e-005 +1Score > 35 indicates identity U X X R.VQDPLEIVGLR.D
2206 +1 492.6317 1474.8733 1474.8548 12.5 0 50 0.00077 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2208 +2 738.4453 1474.8760 1474.8548 14.4 0 69 8.1e-006 +1Score > 31 indicates identity U X X R.QVGVPYIVVFLNK.A
2419 +2 315.7944 1573.9356 1573.9304 3.30 0 14 0.11 +1Score > 30 indicates identity
Score > 17 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2420   525.6527 1573.9363 1573.9304 3.72 0 46 0.00065 +1Score > 30 indicates identity
Score > 27 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2421 -1 394.4915 1573.9369 1573.9304 4.11 0 18 0.72 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F
2418   394.4911 1573.9353 1573.9304 3.10 0 (15) 0.07 +1Score > 30 indicates identity
Score > 16 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F
2423   525.9872 1574.9398 1574.9144 16.1 0 33 0.024 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2424   788.4779 1574.9412 1574.9144 17.0 0 43 0.0026 +1Score > 29 indicates identity U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2425   394.7430 1574.9429 1574.9144 18.1 0 2 1 +1Score > 29 indicates identity
Score > 14 indicates homology		 U X X R.GQVLVKPGSVKPHTK.F + Deamidated (NQ)
2524 +1 538.9687 1613.8843 1613.8665 11.0 1 20 0.11 +1Score > 38 indicates identity
Score > 23 indicates homology		 U X K.LVETLDAYIPEPVR.A
2527 +3 807.9510 1613.8874 1613.8665 13.0 1 92 4.9e-008 +1Score > 38 indicates identity
Score > 31 indicates homology		 U X K.LVETLDAYIPEPVR.A
2555 +2 815.9471 1629.8796 1629.8614 11.2 1 93 2e-007 +1Score > 39 indicates identity
Score > 38 indicates homology		 U X K.LVETLDSYIPEPVR.A
2556   544.3022 1629.8848 1629.8614 14.4 1 30 0.048 +1Score > 38 indicates identity
Score > 30 indicates homology		 U X K.LVETLDSYIPEPVR.A
2805   883.9718 1765.9290 1765.9224 3.77 0 75 1.2e-005 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2806 +1 354.1931 1765.9291 1765.9224 3.81 0 30 0.39 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2807   442.4896 1765.9293 1765.9224 3.91 0 51 0.0011 +1Score > 38 indicates identity
Score > 34 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T
2809   589.6516 1765.9330 1765.9224 5.99 0 43 0.019 +1Score > 38 indicates identity U X X R.SLPHVNVGTIGHVDHGK.T
2811   354.3934 1766.9306 1766.9064 13.7 0 23 0.16 +1Score > 38 indicates identity
Score > 28 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2812 +1 442.7405 1766.9329 1766.9064 15.0 0 44 0.00041 +1Score > 39 indicates identity
Score > 23 indicates homology		 U X X R.SLPHVNVGTIGHVDHGK.T + Deamidated (NQ)
2814   442.9536 1767.7853 1767.7787 3.70 0 16 1 +1Score > 39 indicates identity
Score > 29 indicates homology		 X X R.HYAHVDCPGHADYVK.N
2839   894.9709 1787.9272 1787.9166 5.96 1 51 0.00018 +1Score > 40 indicates identity
Score > 26 indicates homology		 U X R.GITINTAHVEYNSTIR.H
2845   895.4714 1788.9282 1788.9006 15.5 1 59 0.0001 +1Score > 40 indicates identity
Score > 31 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2847 +1 597.3181 1788.9325 1788.9006 17.8 1 48 6.5e-005 +1Score > 40 indicates identity
Score > 18 indicates homology		 U X R.GITINTAHVEYNSTIR.H + Deamidated (NQ)
2862 +1 601.3138 1800.9196 1800.9118 4.30 1 68 9e-005 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H
2873 +1 901.9689 1801.9232 1801.8958 15.2 1 91 4.4e-007 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
2874   601.6486 1801.9240 1801.8958 15.6 1 51 0.0048 +1Score > 40 indicates identity U X R.GITINTAHVEYNSNIR.H + Deamidated (NQ)
3369 +1 712.3776 2134.1110 2134.0769 16.0 1 70 3.7e-005 +1Score > 38 indicates identity U X X R.AIDQPFLMPIEDVFSISGR.G
3485 +2 1097.0718 2192.1290 2192.1551 -11.9 1 44 0.00026 +1Score > 38 indicates identity
Score > 21 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3487 +2 731.7183 2192.1331 2192.1551 -10.1 1 51 7.3e-005 +1Score > 38 indicates identity
Score > 22 indicates homology		 U X R.IIDKPFLMPIEDVFSISGR.G + Oxidation (M)
3603   1133.5771 2265.1396 2265.1165 10.2 0 94 5.2e-009 +1Score > 39 indicates identity
Score > 24 indicates homology		 U X X R.DLLSTYDFPGDDTPIIIGSAR.M

+40 subsets and intersections (417 subset proteins in total)

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 941 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSESM 569 ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 882 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 169 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 206 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 450 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 70 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 115 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 RL1_PSEE4 402 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+6

Accession Score Description
1 RPOC_PSEPK 364 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+7

Accession Score Description
1 DBHB_PSEAE 308 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+8

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 290 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 78 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEP1 287 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
2 RL4_PSE14 192 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 OTCC_PSEPK 280 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
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