MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita4 sp
MS data file : PRT1270_T-BRSC_4_20250714120925.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:41:34 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,786

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 182)

Page: 1 2 3 4 5 6 … 19 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1194 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
3 EFTU_CARRP 163 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1113 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1

+2

Accession Score Description
Family member distances as a dendrogram 1 ATPB_PSEPG 941 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1
2 ATPB_PSESM 569 ATP synthase subunit beta OS=Pseudomonas syringae pv. tomato GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 882 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
3 ATPA_RICAH 169 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
2 ATPA_VEREI 206 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 450 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 70 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 115 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 RL1_PSEE4 402 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+6

Accession Score Description
1 RPOC_PSEPK 364 DNA-directed RNA polymerase subunit beta' OS=Pseudomonas putida (strain KT2440) GN=rpoC PE=3 SV=1

+7

Accession Score Description
1 DBHB_PSEAE 308 DNA-binding protein HU-beta OS=Pseudomonas aeruginosa GN=hupB PE=1 SV=3

+8

Accession Score Description
Family member distances as a dendrogram 1 PORF_PSESY 290 Outer membrane porin F OS=Pseudomonas syringae pv. syringae GN=oprF PE=3 SV=1
2 PORF_PSEAE 78 Outer membrane porin F OS=Pseudomonas aeruginosa GN=oprF PE=1 SV=1

+9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSEP1 287 50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
2 RL4_PSE14 192 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1

-10

Accession Score Description
1 OTCC_PSEPK 280 Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3
Score Mass Matches Sequences emPAI
10.1 OTCC_PSEPK 280 38115 33 (19) 10 (8) 1.14
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas putida (strain KT2440) GN=arcB PE=3 SV=3

-33 peptide matches (19 non-duplicate, 14 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
235 +1 499.3261 498.3188 498.3166 4.55 0 39 0.019 +1Score > 34 indicates identity U R.IAAPK.A
497   345.6850 689.3554 689.3530 3.49 0 33 0.089 +1Score > 43 indicates identity
Score > 35 indicates homology		 K.LGMDVR.I
862   436.2320 870.4494 870.4460 3.95 0 35 0.025 +1Score > 39 indicates identity
Score > 31 indicates homology		 U M.AFNIHNR.N
878 +2 440.2534 878.4922 878.4861 6.95 0 31 0.3 +5Score > 38 indicates identity U R.YLLDLSR.D
880   879.5009 878.4936 878.4861 8.51 0 41 0.022 +1Score > 37 indicates identity U R.YLLDLSR.D
953   916.5015 915.4942 915.4913 3.19 1 56 0.0011 +1Score > 43 indicates identity
Score > 39 indicates homology		 U R.ITLTEDPK.A
954 +1 458.7545 915.4944 915.4913 3.44 1 37 0.058 +1Score > 43 indicates identity
Score > 37 indicates homology		 U R.ITLTEDPK.A
1211 +3 1015.6138 1014.6065 1014.5961 10.3 0 21 0.076 +1Score > 37 indicates identity
Score > 22 indicates homology		 U K.AILVSTLADL.-
1213   508.3114 1014.6082 1014.5961 12.0 0 13 0.086 +1Score > 37 indicates identity
Score > 15 indicates homology		 U K.AILVSTLADL.-
1413   1118.6301 1117.6228 1117.6131 8.67 1 70 4.6e-005 +1Score > 39 indicates identity U K.GNNIALIFEK.T
1414 +1 559.8201 1117.6256 1117.6131 11.2 1 51 0.0015 +1Score > 39 indicates identity
Score > 36 indicates homology		 U K.GNNIALIFEK.T
1636   602.8036 1203.5926 1203.5884 3.53 1 49 0.0017 +1Score > 42 indicates identity
Score > 34 indicates homology		 U K.YTGTEQQHLK.G
1637 -1 402.2052 1203.5938 1203.5884 4.47 1 29 0.02 +1Score > 42 indicates identity
Score > 24 indicates homology		 U K.YTGTEQQHLK.G
1635   402.2043 1203.5911 1203.5884 2.23 1 (27) 0.022 +1Score > 42 indicates identity
Score > 23 indicates homology		 U K.YTGTEQQHLK.G
1903   330.9318 1319.6981 1319.6946 2.66 1 49 0.00051 +1Score > 41 indicates identity
Score > 28 indicates homology		 U R.NLLSLEHHTTR.E
1905 +2 440.9072 1319.6998 1319.6946 3.93 1 42 0.00062 +1Score > 40 indicates identity
Score > 22 indicates homology		 U R.NLLSLEHHTTR.E
1906   660.8574 1319.7002 1319.6946 4.29 1 41 0.0054 +1Score > 40 indicates identity
Score > 31 indicates homology		 U R.NLLSLEHHTTR.E
1908   441.2407 1320.7003 1320.6786 16.4 1 12 1.2 +2Score > 40 indicates identity
Score > 25 indicates homology		 U R.NLLSLEHHTTR.E + Deamidated (NQ)
1959 +1 675.8471 1349.6796 1349.6728 5.08 1 41 0.0041 +1Score > 41 indicates identity
Score > 30 indicates homology		 U K.ALWPHDDLVER.C
1960 +2 450.9009 1349.6809 1349.6728 5.99 1 47 0.00035 +1Score > 41 indicates identity
Score > 25 indicates homology		 U K.ALWPHDDLVER.C

5 subsets and intersections (28 subset proteins in total)

Score Mass Subset of
OTCC_PSEAE 82 38255 10.1
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas aeruginosa GN=arcB PE=1 SV=3
1 sameset of OTCC_PSEAE
OTCC_PSEME 82 38189
Ornithine carbamoyltransferase, catabolic OS=Pseudomonas mendocina GN=arcB PE=3 SV=3
OTCC_CHRVO 59 38025 10.1
Ornithine carbamoyltransferase, catabolic OS=Chromobacterium violaceum GN=arcB PE=3 SV=1
OTCC_HAEIN 41 37908 10.1
Ornithine carbamoyltransferase, catabolic OS=Haemophilus influenzae GN=arcB PE=3 SV=1
3 samesets of OTCC_HAEIN
OTC_HAEIG 41 37881
Ornithine carbamoyltransferase OS=Haemophilus influenzae (strain PittGG) GN=arcB PE=3 SV=1
OTCC_MYCCC 41 35232
Ornithine carbamoyltransferase, catabolic OS=Mycoplasma capricolum subsp. capripneumoniae GN=arcB PE=3 SV=1
THI22_YEAST 41 64521
Thiamine biosynthesis protein THI22 OS=Saccharomyces cerevisiae GN=THI22 PE=2 SV=1
AMN1_PICAN 39 0 10.1
description
19 samesets of AMN1_PICAN
SYA_IGNH4 39 0
description
CIPKB_ORYSJ 39 0
description
UNC53_CAEEL 39 0
description
RS4_DROME 39 0
description
CHDM_DROME 39 0
description
FAS_RAT 39 0
description
GB_MCMVS 39 0
description
MURI_LACAC 39 0
description
OTC_CLOAB 39 0
description
THEG_HUMAN 39 0
description
BOP1_DICDI 39 0
description
DOP1_EMENI 39 0
description
IML1_NEUCR 39 0
description
DNAE2_RALPJ 39 0
description
DNAE2_RALSO 39 0
description
IMPA3_MOUSE 39 0
description
RL3_SPHWW 39 0
description
YHC3_SCHPO 39 0
description
RL32_CARHZ 39 0
description
OTCC_PSEPU 35 4144 10.1
Ornithine carbamoyltransferase, catabolic (Fragment) OS=Pseudomonas putida GN=arcB PE=1 SV=2
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